Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,135,451	A→G	K539K (AAA→AAG)	katG →	catalase‑peroxidase HPI, heme b‑containing

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,135,451	0	A	G	100.0%	166.8 / NA	46	K539K (AAA→AAG)	katG	catalase‑peroxidase HPI, heme b‑containing
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/18); total (28/18)

CGCGTCTGGCATTAATGCCGCAGCGCGACTGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGG  >  NC_000913/4135303‑4135593

cgcgTCTGGCATTAATGCCGCAGCGCGACTGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGc                                                                                                                                              <  2:640037/151‑1 (MQ=255)

       ggCATTAATGCCGCAGCGCGACTGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCa                                                                                                                                          <  2:422192/148‑1 (MQ=255)

       ggCATTAATGCCGCAGCGCGACTGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAgc                                                                                                                                       >  1:617981/1‑151 (MQ=255)

        gCATTAATGCCGCAGCGCGACTGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAgc                                                                                                                                       >  1:93003/1‑150 (MQ=255)

         cATTAATGCCGCAGCGCGACTGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAgcgc                                                                                                                                     >  1:315893/1‑151 (MQ=255)

             aaTGCCGCAGCGCGACTGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCa                                                                                                                                 <  2:66159/151‑1 (MQ=255)

                gCCGCAGCGCGACTGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAgg                                                                                                                               >  2:542595/1‑150 (MQ=255)

                      gcgcGACTGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGc                                                                                                                        <  1:349211/151‑1 (MQ=255)

                        gcgACTGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCAt                                                                                                                      >  2:644069/1‑151 (MQ=255)

                                 aTGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTAcc                                                                                                             >  2:44170/1‑151 (MQ=255)

                                         gccgcAGCCGGGCGTGCGCTGCCTGTTCTGGAGAAAATCCAGAAAGAGGCTGGTAAAGCCTCGCTGGCGGATATCATAGGGCTGGCTGGTGGGGGTGGGTGTGAGAAGGCCGACAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTgcg                                                                                                      >  1:359880/1‑150 (MQ=255)

                                               gCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTc                                                                                               <  2:46546/151‑1 (MQ=255)

                                               gCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTc                                                                                               <  1:63715/151‑1 (MQ=255)

                                                 cGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTcgc                                                                                             <  2:194363/151‑1 (MQ=255)

                                                        gCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATg                                                                                      >  2:586748/1‑151 (MQ=255)

                                                             gCCTGTTCTGGAGAAAATCCAGGAAGAGTCTGGTAACGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGcgcg                                                                                  >  1:656188/1‑150 (MQ=255)

                                                                 gTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCT‑GTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGa                                                                            <  2:520869/151‑1 (MQ=255)

                                                                      ggAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCa                                                                         <  1:4850/150‑1 (MQ=255)

                                                                           aaaTCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTg                                                                    >  2:453818/1‑150 (MQ=255)

                                                                            aaTCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGAc                                                                  >  2:123330/1‑151 (MQ=255)

                                                                             aTCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGAc                                                                  >  1:516913/1‑150 (MQ=255)

                                                                               ccAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACAtt                                                               >  1:382561/1‑151 (MQ=255)

                                                                                cAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTg                                                              >  1:651765/1‑151 (MQ=255)

                                                                                     agggTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATGGAGATg                                                         <  1:211071/148‑1 (MQ=255)

                                                                                     agagTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATg                                                         <  2:452529/151‑1 (MQ=255)

                                                                                        gTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGttt                                                      >  2:142167/1‑151 (MQ=255)

                                                                                            ggTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTg                                                     <  1:15464/148‑1 (MQ=255)

                                                                                            ggTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGa                                                    <  2:540624/149‑1 (MQ=255)

                                                                                            ggTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAgc                                                  >  2:404915/1‑151 (MQ=255)

                                                                                             gTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAgct                                                 >  1:168258/1‑151 (MQ=255)

                                                                                                       gCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGGGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGcc                                        >  1:298415/1‑150 (MQ=255)

                                                                                                       ccTGGCGGATACCATAGTGCTTGCTGGTGTTGTTGGTGTTGAGAAGGCCGCATGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGTCGGGTCGCGCTGATGCGCGTCAGCATCAGACTGACATTGAGATGTTTGGGCTGCTGGGGCCa                                       <  1:224653/150‑1 (MQ=255)

                                                                                                         tGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAAt                                     >  2:169208/1‑151 (MQ=255)

                                                                                                         tGGCGGATATCATAGTGCTGGCTGGCGTGGGTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAAt                                     >  1:431266/1‑151 (MQ=255)

                                                                                                             ggATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGc                                  >  1:1812/1‑150 (MQ=255)

                                                                                                               atatCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTg                                >  2:448803/1‑150 (MQ=255)

                                                                                                                   cATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGt                           >  1:680390/1‑151 (MQ=255)

                                                                                                                       gTGCTGGCTGGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTcc                       <  2:319816/151‑1 (MQ=255)

                                                                                                                         gctggctgGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGt                     <  1:499593/151‑1 (MQ=255)

                                                                                                                         gctggctgGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGt                     >  1:396351/1‑151 (MQ=255)

                                                                                                                         gctggctgGTGTGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGt                     <  1:355505/151‑1 (MQ=255)

                                                                                                                                 gtgtGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATcg             >  1:273873/1‑151 (MQ=255)

                                                                                                                                 gtgtGGTTGGTGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATcg             <  2:622904/151‑1 (MQ=255)

                                                                                                                                     ggttggtGTTGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCt         >  2:211500/1‑151 (MQ=255)

                                                                                                                                             ttGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTgg  >  1:185369/1‑150 (MQ=255)

                                                                                                                                             ttGAGAAGGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTgg  >  2:139533/1‑150 (MQ=255)

CGCGTCTGGCATTAATGCCGCAGCGCGACTGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGG  >  NC_000913/4135303‑4135593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A