Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I3 R1 143 109.6 3434427 98.5% 3382910 150.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,313,236 T→C intergenic (‑58/+106) rbfA ← / ← infB 30s ribosome binding factor/translation initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,313,2360TC97.0% 489.9 / 3.6 135intergenic (‑58/+106)rbfA/infB30s ribosome binding factor/translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base T (4/0);  new base C (66/65);  total (70/65)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01

Only 100 of 135 total aligned reads displayed.

AGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGAATACTTC  >  NC_000913/3313089‑3313383
                                                                                                                                                   |                                                                                                                                                   
aGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGa                                                                                                                                                  <  2:544148/151‑1 (MQ=255)
     cTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGc                                                                                                                                             >  2:271515/1‑151 (MQ=255)
     cTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGc                                                                                                                                             >  2:1343048/1‑151 (MQ=255)
       ttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAggg                                                                                                                                              <  2:602076/148‑1 (MQ=255)
       ttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAgg                                                                                                                                               <  2:1191830/147‑1 (MQ=255)
       ttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAgg                                                                                                                                               <  2:1554926/147‑1 (MQ=255)
       ttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCt                                                                                                                                            <  2:1332129/150‑1 (MQ=255)
       ttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCt                                                                                                                                            <  1:914454/150‑1 (MQ=255)
       ttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCt                                                                                                                                            <  2:750793/150‑1 (MQ=255)
       ttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTg                                                                                                                                           >  1:1445031/1‑151 (MQ=255)
        tAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTg                                                                                                                                           <  2:1043110/150‑1 (MQ=255)
        tAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTg                                                                                                                                           <  1:1704513/150‑1 (MQ=255)
         aaTTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGtt                                                                                                                                         >  1:1024287/1‑151 (MQ=255)
            ttCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCAc                                                                                                                                      >  2:1186464/1‑151 (MQ=255)
              cACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGt                                                                                                                                    >  2:393624/1‑151 (MQ=255)
               aCGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGt                                                                                                                                    <  2:694962/150‑1 (MQ=255)
                cGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTg                                                                                                                                  >  2:392586/1‑151 (MQ=255)
                cGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTg                                                                                                                                  <  2:1307866/151‑1 (MQ=255)
                 gCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTg                                                                                                                                  >  1:1169366/1‑150 (MQ=255)
                  cTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTCTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGAc                                                                                                                                >  1:94490/1‑151 (MQ=255)
                   tGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGAcc                                                                                                                               >  2:312911/1‑151 (MQ=255)
                      aGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCt                                                                                                                              >  1:990922/1‑149 (MQ=255)
                      aGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTg                                                                                                                             >  1:283519/1‑150 (MQ=255)
                         aTGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATa                                                                                                                          >  2:1577082/1‑150 (MQ=255)
                           gagaGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATa                                                                                                                          >  1:371629/1‑148 (MQ=255)
                           gagaGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAg                                                                                                                        <  1:71276/150‑1 (MQ=255)
                            agagCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGAc                                                                                                                      <  1:1186375/151‑1 (MQ=255)
                            agagCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGAc                                                                                                                      >  2:1274906/1‑151 (MQ=255)
                              agCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGAcg                                                                                                                     <  1:112758/150‑1 (MQ=255)
                                      ctTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCa                                                                                                                <  2:1467531/147‑1 (MQ=255)
                                      ctTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAg                                                                                                               <  2:1465362/148‑1 (MQ=255)
                                           tGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTcaca                                                                                                        <  2:1623420/150‑1 (MQ=255)
                                           tGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACAt                                                                                                       >  1:345406/1‑151 (MQ=255)
                                           tGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACAt                                                                                                       >  2:556068/1‑151 (MQ=255)
                                                ttCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAgg                                                                                                   <  1:274112/150‑1 (MQ=255)
                                                 tCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGACACATCAGGc                                                                                                  >  2:587977/1‑150 (MQ=255)
                                                      cgcTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATcc                                                                                             >  1:513263/1‑150 (MQ=255)
                                                      cgcTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATcc                                                                                             >  1:1475605/1‑150 (MQ=255)
                                                        cTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCAt                                                                                           >  2:716358/1‑150 (MQ=255)
                                                         tACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGc                                                                                         >  2:571583/1‑151 (MQ=255)
                                                         tACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGc                                                                                         >  1:198222/1‑151 (MQ=255)
                                                          aCGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGcc                                                                                        >  1:1353498/1‑151 (MQ=255)
                                                           cgcgCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCg                                                                                       >  2:155826/1‑151 (MQ=255)
                                                             cgcTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCgg                                                                                      >  1:219643/1‑150 (MQ=255)
                                                             cgcTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCgg                                                                                      >  1:1614591/1‑150 (MQ=255)
                                                              gcTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGAt                                                                                    >  2:658342/1‑151 (MQ=255)
                                                                tGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGc                                                                                  >  2:1121257/1‑151 (MQ=255)
                                                                    ggCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCg                                                                              >  2:917798/1‑151 (MQ=255)
                                                                     gCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGc                                                                               >  1:1468546/1‑149 (MQ=255)
                                                                     gCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGc                                                                               <  2:1468644/149‑1 (MQ=255)
                                                                      cGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTa                                                                            <  2:470001/151‑1 (MQ=255)
                                                                       gACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTa                                                                            >  1:710140/1‑150 (MQ=255)
                                                                         ccAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAAc                                                                         <  2:1568787/151‑1 (MQ=255)
                                                                          cAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAAc                                                                         >  2:1089613/1‑150 (MQ=255)
                                                                           aaaTTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGc                                                                       <  1:1090215/151‑1 (MQ=255)
                                                                            aaTTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGcc                                                                      >  2:554570/1‑151 (MQ=255)
                                                                             aTTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCt                                                                     >  1:1335010/1‑151 (MQ=255)
                                                                               tCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCtt                                                                    <  1:35746/150‑1 (MQ=255)
                                                                                  ttCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTa                                                                   <  2:1025477/148‑1 (MQ=255)
                                                                                  ttCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTa                                                                   <  1:1518124/148‑1 (MQ=255)
                                                                                  ttCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTAt                                                                  <  1:21793/149‑1 (MQ=255)
                                                                                    cGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATccc                                                               <  2:1515611/150‑1 (MQ=255)
                                                                                     gCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGc                                                             >  1:361446/1‑151 (MQ=255)
                                                                                      ccATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCa                                                            <  1:1691477/151‑1 (MQ=255)
                                                                                       cATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCAt                                                           >  2:380867/1‑151 (MQ=255)
                                                                                          aataaATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCAt                                                           <  1:126756/148‑1 (MQ=255)
                                                                                          aataaATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATgg                                                         <  1:762063/150‑1 (MQ=255)
                                                                                          aataaATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATgg                                                         <  2:1445129/150‑1 (MQ=255)
                                                                                          aataaATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATgg                                                         <  1:1603008/150‑1 (MQ=255)
                                                                                          aataaATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATgg                                                         <  1:1018065/150‑1 (MQ=255)
                                                                                              aaTTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAAccc                                                    <  1:1393379/151‑1 (MQ=255)
                                                                                               aTTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCt                                                   <  1:1078319/151‑1 (MQ=255)
                                                                                                  ctcCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTaa                                                 >  1:1694897/1‑150 (MQ=255)
                                                                                                  ctcCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTaa                                                 >  1:1573352/1‑150 (MQ=255)
                                                                                                    ccTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTaaaa                                               <  2:948343/150‑1 (MQ=255)
                                                                                                    ccTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTaa                                                 <  2:345273/148‑1 (MQ=255)
                                                                                                       gACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCt                                           >  2:1180793/1‑151 (MQ=255)
                                                                                                          aaaaaaGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTaa                                        <  2:1507096/151‑1 (MQ=255)
                                                                                                          aaaaaaGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTaa                                        <  2:1519042/151‑1 (MQ=255)
                                                                                                          aaaaaaGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTa                                         >  1:1021156/1‑150 (MQ=255)
                                                                                                          aaaaaaGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTa                                         >  1:1246657/1‑150 (MQ=255)
                                                                                                             aaaGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGc                                      >  1:742369/1‑150 (MQ=255)
                                                                                                               aGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAAt                                   <  2:1624751/151‑1 (MQ=255)
                                                                                                               aGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAAt                                   >  1:687933/1‑151 (MQ=255)
                                                                                                                ggggCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATg                                  <  2:129127/151‑1 (MQ=255)
                                                                                                                     tGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACg                             >  2:1416393/1‑151 (MQ=255)
                                                                                                                     tGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACg                             >  1:1245345/1‑151 (MQ=255)
                                                                                                                       ttAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGt                            <  1:1274811/150‑1 (MQ=255)
                                                                                                                         aGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTgg                         <  1:857662/151‑1 (MQ=255)
                                                                                                                         aGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTg                          <  2:469106/150‑1 (MQ=255)
                                                                                                                            cccTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCa                                     <  1:575841/136‑1 (MQ=255)
                                                                                                                                  ttAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCgatg                >  1:1340175/1‑151 (MQ=255)
                                                                                                                                  ttAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCgat                 <  1:312884/150‑1 (MQ=255)
                                                                                                                                   tAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCgatg                <  2:238270/150‑1 (MQ=255)
                                                                                                                                      aattaattTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGAttt            <  2:1320806/151‑1 (MQ=255)
                                                                                                                                        ttaattTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCg          >  1:1627696/1‑151 (MQ=255)
                                                                                                                                         taattTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCg          <  1:1114882/150‑1 (MQ=255)
                                                                                                                                          aattTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGaa        <  2:283544/151‑1 (MQ=255)
                                                                                                                                          aattTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGa         <  2:1120845/150‑1 (MQ=255)
                                                                                                                                             ttCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGAATAc     >  1:154742/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAGCAATGGTACGTTGGATCTCGATGATTTCGAATACTTC  >  NC_000913/3313089‑3313383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A