Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,314,066	G→A	A650V (GCG→GTG)	infB ←	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,314,066	0	G	A	100.0%	115.7 / NA	31	A650V (GCG→GTG)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/17); total (14/17)

TGTACGTCTGCCTTCAGGACGATATTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCGCTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGACCCAGTTCGTTACGCATCGCACGAACACGACCGT  >  NC_000913/3313922‑3314207

tGTACGTCTGCCTTCAGGACGATATTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTttct                                                                                                                                         >  1:510928/1‑151 (MQ=255)

 gTACGTCTGCCTTCAGGACGATATTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTttct                                                                                                                                         <  1:334410/150‑1 (MQ=255)

     gTCTGCCTTCAGGACGATATTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTATCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCg                                                                                                                                    <  1:404287/151‑1 (MQ=255)

                  aCGATATTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACgg                                                                                                                       >  2:211416/1‑151 (MQ=255)

                     atatTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTaa                                                                                                                    <  1:122176/151‑1 (MQ=255)

                      tatTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTaa                                                                                                                    >  2:122607/1‑150 (MQ=255)

                              tCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTtcatc                                                                                                             <  1:98382/149‑1 (MQ=255)

                                gTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCAccc                                                                                                         <  2:570315/151‑1 (MQ=255)

                                         gCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGt                                                                                                <  1:469038/151‑1 (MQ=255)

                                             tCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACg                                                                                             <  2:25764/150‑1 (MQ=255)

                                                gTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCgg                                                                                         <  2:616527/151‑1 (MQ=255)

                                                 tCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCgg                                                                                         >  2:360523/1‑150 (MQ=255)

                                                     gTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAgg                                                                                    <  2:537793/151‑1 (MQ=255)

                                                       tGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGAAACTTCATCACCCGCAGCCGGTACGCCGGACAGGcc                                                                                  >  2:20077/1‑151 (MQ=255)

                                                                tGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTc                                                                         >  1:544645/1‑151 (MQ=255)

                                                                          tttAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATg                                                               >  2:202315/1‑151 (MQ=255)

                                                                              gATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATggacg                                                           >  1:457804/1‑151 (MQ=255)

                                                                                  tCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGAcc                                                       >  2:490674/1‑151 (MQ=255)

                                                                                       tgACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCtt                                                  >  2:438225/1‑151 (MQ=255)

                                                                                        gACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCt                                                   <  1:430024/149‑1 (MQ=255)

                                                                                         aCGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTcc                                                >  2:37210/1‑151 (MQ=255)

                                                                                             gcCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCa                                            <  2:371928/151‑1 (MQ=255)

                                                                                              ccAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCGCCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCAc                                           <  1:533669/151‑1 (MQ=255)

                                                                                                 gTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTc                                        >  1:625288/1‑151 (MQ=255)

                                                                                                      aCTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGAc                                   <  2:166658/151‑1 (MQ=255)

                                                                                                         tCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGAccc                                 <  1:56352/150‑1 (MQ=255)

                                                                                                          cgcgGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGAccc                                 >  1:458457/1‑149 (MQ=255)

                                                                                                             ggAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGACCCAGTTc                            <  2:230536/151‑1 (MQ=255)

                                                                                                                  ttACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGACCCAGTTCGTTa                        <  1:166752/150‑1 (MQ=255)

                                                                                                                                 agTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGACCCAGTTCGTTACGCATCGCACGAacac        <  2:457465/151‑1 (MQ=255)

                                                                                                                                        cTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGACCCAGTTCGTTACGCATCGCACGAACACGACCGt  >  1:277394/1‑150 (MQ=255)

TGTACGTCTGCCTTCAGGACGATATTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCGCTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGACCCAGTTCGTTACGCATCGCACGAACACGACCGT  >  NC_000913/3313922‑3314207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A