Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,319,794	T→C	R386R (CGT→CGC)	argG →	argininosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,319,794	0	T	C	100.0%	114.8 / NA	32	R386R (CGT→CGC)	argG	argininosuccinate synthetase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/16); total (16/16)

TGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCAGG  >  NC_000913/3319647‑3319940

tGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTg                                                                                                                                                 >  2:249623/1‑151 (MQ=255)

         ggCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGa                                                                                                                                         >  2:255423/1‑150 (MQ=255)

           cgcTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGaaaa                                                                                                                                      <  2:516065/151‑1 (MQ=255)

             cTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGaaaa                                                                                                                                      >  2:610512/1‑149 (MQ=255)

                aTGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCg                                                                                                                                 <  2:393048/151‑1 (MQ=255)

                     gcGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACt                                                                                                                              <  1:255430/149‑1 (MQ=255)

                          aCTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGGAAAAGGCGACTCGGTGtt                                                                                                                       >  1:602310/1‑151 (MQ=255)

                            tctctGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTtct                                                                                                                     >  2:57431/1‑151 (MQ=255)

                              tctGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCg                                                                                                                   >  2:41733/1‑151 (MQ=255)

                               ctGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTtctc                                                                                                                    <  2:24719/149‑1 (MQ=255)

                               ctGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTtct                                                                                                                     <  1:607477/148‑1 (MQ=255)

                               ctGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTtct                                                                                                                     <  2:313989/148‑1 (MQ=255)

                                  cAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGcc                                                                                                                 >  2:557004/1‑149 (MQ=255)

                                                          aCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCa                                                                                       <  1:596764/151‑1 (MQ=255)

                                                             ggTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGc                                                                                    <  2:285969/151‑1 (MQ=255)

                                                                 aaGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTa                                                                                 >  1:77148/1‑150 (MQ=255)

                                                                      aCCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTgg                                                                           <  2:105401/151‑1 (MQ=255)

                                                                             aGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCAc                                                                    >  2:315691/1‑151 (MQ=255)

                                                                                  cgcCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATa                                                               <  2:297485/151‑1 (MQ=255)

                                                                                     cGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATAccc                                                            <  2:631803/151‑1 (MQ=255)

                                                                                           aaCGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCcgcg                                                         >  1:370850/1‑148 (MQ=255)

                                                                                                 tattCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACttt                                                >  2:583671/1‑151 (MQ=255)

                                                                                                     cAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCgg                                            <  2:485181/151‑1 (MQ=255)

                                                                                                       aTCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGtt                                          >  2:147721/1‑151 (MQ=255)

                                                                                                         ccTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTa                                         <  2:368819/150‑1 (MQ=255)

                                                                                                                aCCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCaaa                                  <  2:141000/150‑1 (MQ=255)

                                                                                                                      tcAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGcc                           >  1:523694/1‑151 (MQ=255)

                                                                                                                                tGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTtcctc                 >  1:139991/1‑151 (MQ=255)

                                                                                                                                            cAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTc     >  1:512892/1‑151 (MQ=255)

                                                                                                                                            cAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTc     >  2:419361/1‑151 (MQ=255)

                                                                                                                                             agagCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCa    <  2:185912/151‑1 (MQ=255)

                                                                                                                                               agCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCAgg  <  2:224939/151‑1 (MQ=255)

TGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCAGG  >  NC_000913/3319647‑3319940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A