Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I3 R1 143 109.6 3434427 98.5% 3382910 150.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,377,215 A→G L69P (CTG→CCG)  sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,377,2150AG99.1% 400.5 / NA 115L69P (CTG→CCG) sspAstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (69/45);  minor base C (1/0);  total (70/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 118 total aligned reads displayed.

ATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTAT  >  NC_000913/3377068‑3377359
                                                                                                                                                   |                                                                                                                                                
  gatgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTc                                                                                                                                             <  2:1364881/151‑1 (MQ=255)
   atgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTc                                                                                                                                             >  1:1231858/1‑150 (MQ=255)
   atgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCa                                                                                                                                            <  1:339842/151‑1 (MQ=255)
      gtgtTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGc                                                                                                                                          >  2:1385953/1‑150 (MQ=255)
      gtgtTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCt                                                                                                                                         <  1:376886/151‑1 (MQ=255)
         ttCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCCTCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCAc                                                                                                                                      >  1:362812/1‑151 (MQ=255)
         ttCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCAc                                                                                                                                      >  2:18297/1‑151 (MQ=255)
          tcatcaGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCAc                                                                                                                                      >  1:131683/1‑150 (MQ=255)
           catcaGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGa                                                                                                                                    <  1:1702639/151‑1 (MQ=255)
             tcaGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGAAATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATc                                                                                                                                  >  2:1687857/1‑151 (MQ=255)
              caGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATcc                                                                                                                                 >  2:335989/1‑151 (MQ=255)
               aGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGGGATTCCCACGGGGTCAGCTCACGATcc                                                                                                                                 >  2:1698656/1‑150 (MQ=255)
                    gtACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAgg                                                                                                                           <  2:618990/151‑1 (MQ=255)
                     tACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCAAGATCCACCAggg                                                                                                                          >  2:235539/1‑151 (MQ=255)
                      aCCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCCGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGGCAGCCCACCATCCACCAGGGt                                                                                                                         >  2:1470399/1‑151 (MQ=255)
                          gTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCgg                                                                                                                      >  2:624482/1‑150 (MQ=255)
                           tCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCCGCTCACGATCCACCAGGGTCgg                                                                                                                      >  2:1526422/1‑149 (MQ=255)
                           tCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGa                                                                                                                     >  1:749472/1‑150 (MQ=255)
                           tCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGGCAGCTCACGATCCACCAGGGTCgg                                                                                                                      >  2:1205673/1‑149 (MQ=255)
                             tttttCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACg                                                                                                                  >  1:798114/1‑151 (MQ=255)
                             tttttCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACCCTCATCCAGATATTCCCTAATGATGCGAGATTCCCCCGGGGTCACCTCACGCTCCACCCGGGt                                                                                                                         >  2:1079117/1‑144 (MQ=255)
                              ttttCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGc                                                                                                                 >  1:1661480/1‑151 (MQ=255)
                              ttttCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGGCAGCTCACGATCCACCAGGGTCGGAAc                                                                                                                   >  1:84196/1‑149 (MQ=255)
                                 tCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGctct                                                                                                              >  1:26016/1‑151 (MQ=255)
                                    atgcgatgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGAt                                                                                                           >  1:460273/1‑151 (MQ=255)
                                    atgcgatgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGAt                                                                                                           <  2:1494185/151‑1 (MQ=255)
                                      gcgatgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTc                                                                                                         <  1:74793/151‑1 (MQ=255)
                                       cgatgaATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCCCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCCCCAGGGTCGGAACGCTCTGAtt                                                                                                          >  1:1082475/1‑149 (MQ=255)
                                         atgcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCggg                                                                                                      >  1:992046/1‑151 (MQ=255)
                                           gcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGt                                                                                                     <  2:229030/150‑1 (MQ=255)
                                            cATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTg                                                                                                   <  1:1116930/151‑1 (MQ=255)
                                             aTGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGGGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGGACGCTCTGATTCggggt                                                                                                    >  1:1562339/1‑147 (MQ=255)
                                             aTGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGa                                                                                                  <  2:373405/151‑1 (MQ=255)
                                                tACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGTGGATACGGGAAACGCTCATGCAAATATTCCATAAATATTTGTGAATCCCACCGGGGCGGCTCGCGATCCACCAGGGTCGGAAACCTCTGATTTGGGTTgtg                                                                                                 >  1:199650/1‑147 (MQ=255)
                                                 aCAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTc                                                                                              <  2:1081155/151‑1 (MQ=255)
                                                  cAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCa                                                                                             <  2:1476841/151‑1 (MQ=255)
                                                  cAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGGTGAGGTc                                                                                              >  1:569120/1‑150 (MQ=255)
                                                        gCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGa                                                                                       <  1:1547028/151‑1 (MQ=255)
                                                            taaCCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCCTGCAGATATTTCATAATGATGGGAGATTCCCACGGGGTCAACTTTCGGTCCACCAGGGCCGGGACGCTTTGTTTCGGGTTGGGGACTATCAGAtac                                                                                    >  1:419723/3‑148 (MQ=255)
                                                              aCCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGa                                                                                 <  2:403403/151‑1 (MQ=255)
                                                               ccGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCCCCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGa                                                                                 <  1:1204231/150‑1 (MQ=255)
                                                               ccGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAg                                                                                <  2:602574/151‑1 (MQ=255)
                                                               ccGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGAGGGAAACGCTCAGCCAGATATTCCATAATAATTCGAGATTCCCAAGGGGTCAGCTCACAAACCAACAGGGTCGGGACTCCCTGCTTCCGGTTGTGGACAAACCGAt                                                                                      >  1:619060/1‑145 (MQ=255)
                                                                 gcgAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTTCATAATGATGCGGGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAAACCTCTGGTTCGGGCTGAGGGCAATCAGATCCTGAGGc                                                                              >  2:697547/1‑151 (MQ=255)
                                                                 gcgAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGc                                                                              <  1:802506/151‑1 (MQ=255)
                                                                   gAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCgg                                                                            <  1:182198/151‑1 (MQ=255)
                                                                   gAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCgg                                                                            >  1:30340/1‑151 (MQ=255)
                                                                   gAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGGCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCgg                                                                            >  2:1104848/1‑151 (MQ=255)
                                                                        aCCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGt                                                                       <  1:1537071/151‑1 (MQ=255)
                                                                           gggTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCt                                                                    <  2:973349/151‑1 (MQ=255)
                                                                           gggTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCt                                                                    >  2:242364/1‑151 (MQ=255)
                                                                                 aCAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCa                                                              <  2:750481/151‑1 (MQ=255)
                                                                                  cAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCAc                                                             >  2:1671438/1‑151 (MQ=255)
                                                                                   aGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACg                                                            >  2:463451/1‑151 (MQ=255)
                                                                                      cATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCCCGGGGTCCGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACgtg                                                          >  1:576972/1‑150 (MQ=255)
                                                                                       aTCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGtt                                                        <  1:1200340/151‑1 (MQ=255)
                                                                                       aTCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGtt                                                        <  1:850219/151‑1 (MQ=255)
                                                                                       aTCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGtt                                                        >  1:1020150/1‑151 (MQ=255)
                                                                                        tCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGtt                                                        <  1:1470301/150‑1 (MQ=255)
                                                                                          aGTGGCGGATGGGGGGAAAGCTCATCCCGATATTTCCTAATGATGCGGGATTCCCCCGGGGGCCGCCCACGATCCACCAGGGTCGGGACGCTCTGATTCGGGGTGAGGTCAATCAGATCCTGAGGCGGGTTGTTCTTTTTCCCGTGtt                                                        >  1:1536309/1‑148 (MQ=255)
                                                                                           gTGGCGGATGCGGGGAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGGCGGGACGCTCTGATTCGGGTTGAGGTCAATTAGATCCTGAGGGGGATTGTCCTTTTCCACGTGTTCGa                                                     >  1:338119/1‑150 (MQ=255)
                                                                                               cGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCg                                                <  2:598178/151‑1 (MQ=255)
                                                                                                   tGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAAc                                            >  2:1156618/1‑151 (MQ=255)
                                                                                                   tGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAAc                                            >  1:402458/1‑151 (MQ=255)
                                                                                                    gCGGGAAACGCTCATCCAGATATGCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGATGTCAATCAGATCCTGAGGCGGATTGTCCGTTTCCACGTGTTCGATCTCGAAACt                                           <  1:1636504/151‑1 (MQ=255)
                                                                                                     cGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACtt                                          >  2:101373/1‑151 (MQ=255)
                                                                                                      gggAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACtt                                          >  1:99446/1‑150 (MQ=255)
                                                                                                       ggAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTac                                        >  2:613790/1‑151 (MQ=255)
                                                                                                        gAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTac                                        >  2:977637/1‑150 (MQ=255)
                                                                                                        gAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTac                                        >  2:737519/1‑150 (MQ=255)
                                                                                                          aaCGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAGACTTACAcc                                     >  2:1598783/1‑151 (MQ=255)
                                                                                                           aCGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCt                                    >  2:489476/1‑151 (MQ=255)
                                                                                                              cTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTtc                                 >  1:438631/1‑151 (MQ=255)
                                                                                                               tCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTtct                                >  2:1701243/1‑151 (MQ=255)
                                                                                                                cATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTtctc                               <  1:598631/151‑1 (MQ=255)
                                                                                                                cATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTtct                                >  2:977297/1‑150 (MQ=255)
                                                                                                                 aTCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTtctc                               >  2:934006/1‑150 (MQ=255)
                                                                                                                 aTCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTtctc                               >  2:1634429/1‑150 (MQ=255)
                                                                                                                   ccAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTcag                             <  2:447349/150‑1 (MQ=255)
                                                                                                                    cAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTcagcc                           >  1:1352355/1‑151 (MQ=255)
                                                                                                                     aGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTcagcc                           >  1:912985/1‑150 (MQ=255)
                                                                                                                      gATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTcagccag                         <  2:381728/151‑1 (MQ=255)
                                                                                                                      gATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTcagcc                           >  1:110088/1‑149 (MQ=255)
                                                                                                                      gATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTcagcc                           >  1:715356/1‑149 (MQ=255)
                                                                                                                         atTCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGca                       <  1:1694833/150‑1 (MQ=255)
                                                                                                                           tCCATAATGATGCGAGATTCCCACGGGGTCAGCTCTCGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGcaca                     >  1:1334567/1‑150 (MQ=255)
                                                                                                                           tCCATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGcaca                     <  1:228951/150‑1 (MQ=255)
                                                                                                                            ccATAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAAt                   >  2:94489/1‑151 (MQ=255)
                                                                                                                               tAATGATGCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCg                >  1:289460/1‑151 (MQ=255)
                                                                                                                                 atgatgCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCgg               >  1:1324743/1‑150 (MQ=255)
                                                                                                                                   gatgCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGAcc            >  2:306881/1‑151 (MQ=255)
                                                                                                                                    atgCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCt           <  2:1661588/151‑1 (MQ=255)
                                                                                                                                      gCGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTg          <  1:267551/150‑1 (MQ=255)
                                                                                                                                       cGAGATTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGa         >  2:989429/1‑150 (MQ=255)
                                                                                                                                        gagaTTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATg       >  1:766902/1‑151 (MQ=255)
                                                                                                                                        gagaTTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATg       <  2:1503670/151‑1 (MQ=255)
                                                                                                                                           aTTCCCACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCt    >  1:763828/1‑151 (MQ=255)
                                                                                                                                              cccACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCtat  <  2:902084/150‑1 (MQ=255)
                                                                                                                                               ccACGGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCta   >  2:721303/1‑148 (MQ=255)
                                                                                                                                                cacgGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCtat  >  1:1301278/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
ATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTAT  >  NC_000913/3377068‑3377359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A