Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F50 I1 R1 141 76.7 2402765 98.4% 2364320 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,695,244 T→C L100L (CTA→CTG)  purL ← phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,695,2440TC95.3% 220.0 / 1.3 64L100L (CTA→CTG) purLphosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (29/32);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

ACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGC  >  NC_000913/2695096‑2695391
                                                                                                                                                    |                                                                                                                                                   
acaacTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAg                                                                                                                                                   <  2:733085/151‑1 (MQ=255)
 caacTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGc                                                                                                                                                  >  1:284178/1‑151 (MQ=255)
  aacTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGcc                                                                                                                                                 >  1:282269/1‑151 (MQ=255)
      gCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGc                                                                                                                                              <  2:389357/150‑1 (MQ=255)
      gCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGc                                                                                                                                              <  2:6764/150‑1 (MQ=255)
        tctGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGt                                                                                                                                           >  2:983183/1‑151 (MQ=255)
           gCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTGCCTGTTGCAGCCCGCAGTTa                                                                                                                                         <  1:442151/150‑1 (MQ=255)
           gCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGtt                                                                                                                                          <  2:891868/149‑1 (MQ=255)
           gCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGtt                                                                                                                                          <  1:24603/149‑1 (MQ=255)
           gCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTAt                                                                                                                                        >  1:1107667/1‑151 (MQ=255)
             atcatcTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATgg                                                                                                                                      <  2:401671/151‑1 (MQ=255)
             atcatcTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATgg                                                                                                                                      >  1:1104598/1‑151 (MQ=255)
                atcTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGc                                                                                                                                    >  1:373492/1‑150 (MQ=255)
                  cTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAat                                                                                                                                 >  2:526781/1‑151 (MQ=255)
                    aaaGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAatat                                                                                                                               <  1:925116/151‑1 (MQ=255)
                    aaaGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAata                                                                                                                                <  2:795790/150‑1 (MQ=255)
                           aaaaGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGc                                                                                                                        >  1:1174550/1‑151 (MQ=255)
                             aaGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCtt                                                                                                                      <  2:185556/151‑1 (MQ=255)
                              aGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCttt                                                                                                                     >  2:1179037/1‑151 (MQ=255)
                               gACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTc                                                                                                                    >  1:1003257/1‑151 (MQ=255)
                                     ttCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGa                                                                                                               <  1:568705/150‑1 (MQ=255)
                                      tCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGAc                                                                                                              >  1:432063/1‑150 (MQ=255)
                                         atcatGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAgg                                                                                                          <  2:109580/151‑1 (MQ=255)
                                           catGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGga                                                                                                        <  2:198172/151‑1 (MQ=255)
                                                 gTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATg                                                                                                  <  1:457405/151‑1 (MQ=255)
                                                    gTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTg                                                                                               >  2:526949/1‑151 (MQ=255)
                                                       cAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGcc                                                                                             >  1:84862/1‑150 (MQ=255)
                                                       cAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCa                                                                                            <  2:1006975/151‑1 (MQ=255)
                                                           tCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGa                                                                                         <  2:1126747/150‑1 (MQ=255)
                                                                 gTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCgggg                                                                                  <  2:500364/151‑1 (MQ=255)
                                                                    aCCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCa                                                                               <  2:1083165/151‑1 (MQ=255)
                                                                        gctgcCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACca                                                                            <  2:1104626/150‑1 (MQ=255)
                                                                        gctgcCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACca                                                                            <  2:1154132/150‑1 (MQ=255)
                                                                          tgcCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACcagc                                                                          >  2:137429/1‑150 (MQ=255)
                                                                          tgcCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACcagc                                                                          >  1:633575/1‑150 (MQ=255)
                                                                           gcCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACcagcag                                                                        <  2:754466/151‑1 (MQ=255)
                                                                           gcCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACcagca                                                                         <  2:70190/150‑1 (MQ=255)
                                                                           gcCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACcagca                                                                         <  1:771343/150‑1 (MQ=255)
                                                                             cATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAgg                                                                       >  1:179095/1‑150 (MQ=255)
                                                                             cATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAgg                                                                       >  1:260840/1‑150 (MQ=255)
                                                                             cATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAgg                                                                       >  1:188882/1‑150 (MQ=255)
                                                                                 gTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGttt                                                                  <  1:412077/151‑1 (MQ=255)
                                                                                    cATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGcc                                                               >  2:386699/1‑151 (MQ=255)
                                                                                         gTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCtt                                                             <  2:1009153/148‑1 (MQ=255)
                                                                                         gTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCg                                                          >  2:254023/1‑151 (MQ=255)
                                                                                           cAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCggg                                                        >  2:346790/1‑151 (MQ=255)
                                                                                               gTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGt                                                    <  2:942120/151‑1 (MQ=255)
                                                                                                 aCCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGtgg                                                  <  2:1184323/151‑1 (MQ=255)
                                                                                                   cGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGtggct                                                <  2:522112/151‑1 (MQ=255)
                                                                                                        tCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGtggctggc                                             <  1:1046850/149‑1 (MQ=255)
                                                                                                        tCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCg                                            <  2:970662/150‑1 (MQ=255)
                                                                                                            tataGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGc                                       >  1:875645/1‑151 (MQ=255)
                                                                                                            tataGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGc                                       >  2:875602/1‑151 (MQ=255)
                                                                                                             ataGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGcc                                      >  1:102557/1‑151 (MQ=255)
                                                                                                             ataGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGcc                                      >  1:183006/1‑151 (MQ=255)
                                                                                                                    gCAACGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGGGGCTGGCGAGTGCCGGGCCa                                >  1:645918/1‑150 (MQ=255)
                                                                                                                       aCGCCGCGCTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATAtt                            >  1:679198/1‑151 (MQ=255)
                                                                                                                            gcgcTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTcagc                       >  2:728505/1‑151 (MQ=255)
                                                                                                                             cgcTCAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTcagca                      <  2:163997/151‑1 (MQ=255)
                                                                                                                                 cAAGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGc                   <  2:855819/150‑1 (MQ=255)
                                                                                                                                  aaGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGt                 >  1:1067709/1‑151 (MQ=255)
                                                                                                                                  aaGGCGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGt                 >  1:907797/1‑151 (MQ=255)
                                                                                                                                      cGGTTTACCTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCaa             <  2:252738/151‑1 (MQ=255)
                                                                                                                                             ccTGTTGCAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTgtgc      >  2:973962/1‑151 (MQ=255)
                                                                                                                                                 ttgcAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTgtgcgtgc  >  1:787074/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCATCATGCGGTCGTGCAGTTCAGCGGTAACCTGCTGCCATTGTTCATTGGTCAGCGTACCGGCTTCTATATAGTAAGCAACGCCGCGCTCAAGGCGGTTTACCTGTTGTAGCCCGCAGTTATGGGCAATATCGGTCGCTTTCGAAGACCAGGGAGAGATGGTGCCAGGACGCGGGGTCACCAGCAGGAGTTTGCCTTGCGGGGCGTGGCTGGCGAGTGCCGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGC  >  NC_000913/2695096‑2695391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A