Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	277,958	A→G	L282P (CTC→CCC)	afuC ←	CP4‑6 prophage; putative ferric transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	277,958	0	A	G	100.0%	126.4 / NA	37	L282P (CTC→CCC)	afuC	CP4‑6 prophage; putative ferric transporter subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/20); total (17/20)

CTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGAGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCACCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAA  >  NC_000913/277810‑278096

cTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGc                                                                                                                                          >  2:464274/1‑151 (MQ=255)

  gCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGc                                                                                                                                          <  1:562401/149‑1 (MQ=255)

     cGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGAt                                                                                                                                     >  2:503006/1‑151 (MQ=255)

      gCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATc                                                                                                                                    >  1:243474/1‑151 (MQ=255)

      gCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATc                                                                                                                                    <  1:458090/151‑1 (MQ=255)

          aCGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCtt                                                                                                                                >  1:520311/1‑151 (MQ=255)

                ggTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGgc                                                                                                                          >  2:206747/1‑151 (MQ=255)

                      aGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCacac                                                                                                                    <  2:472707/151‑1 (MQ=255)

                       gACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACAcc                                                                                                                   >  1:435646/1‑151 (MQ=255)

                                cGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCGTATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATccc                                                                                                          <  1:155747/151‑1 (MQ=255)

                                cGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATccc                                                                                                          <  1:39051/151‑1 (MQ=255)

                                       cTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGccc                                                                                                   >  1:98323/1‑151 (MQ=255)

                                          cAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCtgt                                                                                                <  1:265317/151‑1 (MQ=255)

                                          cAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCtgt                                                                                                >  1:333350/1‑151 (MQ=255)

                                              aataTCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTAc                                                                                            >  2:534676/1‑151 (MQ=255)

                                                              gCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGgcg                                                                            >  1:283822/1‑151 (MQ=255)

                                                              gCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGgc                                                                             <  2:97286/150‑1 (MQ=255)

                                                                   tCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGc                                                                        >  2:36591/1‑150 (MQ=255)

                                                                      aCCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGc                                                                        >  1:177288/1‑147 (MQ=255)

                                                                             cTTCATACTGCGGCCCCATATATGCGACATGGCGGGTCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACCCCGACCATCCCATCGCCCTGTGTACCAAAGTGCAGCGGGCGCTGCAGAGGATa                                                                <  2:610357/148‑1 (MQ=255)

                                                                               tCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCg                                                            <  1:493971/150‑1 (MQ=255)

                                                                                 aTACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAg                                                         <  2:133143/151‑1 (MQ=255)

                                                                                     tGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGatat                                                     >  2:130724/1‑151 (MQ=255)

                                                                                         gCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAAc                                                 <  1:213766/151‑1 (MQ=255)

                                                                                               tataGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATcc                                           >  2:50380/1‑151 (MQ=255)

                                                                                                 taGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGt                                         >  2:98569/1‑151 (MQ=255)

                                                                                                     cGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTATCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCt                                     <  1:226646/151‑1 (MQ=255)

                                                                                                      gACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTg                                    <  2:338362/151‑1 (MQ=255)

                                                                                                         aTGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAg                                 <  2:65459/151‑1 (MQ=255)

                                                                                                         aTGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAg                                 <  1:176271/151‑1 (MQ=255)

                                                                                                         aTGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAg                                 <  1:406005/151‑1 (MQ=255)

                                                                                                               gATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGcc                           <  1:98569/151‑1 (MQ=255)

                                                                                                                aTCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCg                          >  1:615280/1‑151 (MQ=255)

                                                                                                                   aCGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATGTCAACGTATCCGTCGCTGAAGGTTGCCGGGa                       <  1:572949/151‑1 (MQ=255)

                                                                                                                     gCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAAc                     <  1:8124/151‑1 (MQ=255)

                                                                                                                             ggCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTg               <  2:572740/149‑1 (MQ=255)

                                                                                                                                          gcgATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATaa  >  1:73038/1‑149 (MQ=255)

CTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGAGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCACCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAA  >  NC_000913/277810‑278096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A