Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,803,836	T→C	L112P (CTA→CCA)	nrdF →	ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,803,836	0	T	C	97.4%	136.7 / ‑3.9	39	L112P (CTA→CCA)	nrdF	ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (17/21); total (17/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01

ACGCTGCTCGACACGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTT  >  NC_000913/2803691‑2803980

aCGCTGCTCGACACGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGcc                                                                                                                                             >  1:173979/1‑151 (MQ=255)

     gctCGACACGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGAcc                                                                                                                                        >  2:516483/1‑151 (MQ=255)

        cGACACGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCaa                                                                                                                                      <  2:472842/150‑1 (MQ=255)

          acacGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGa                                                                                                                                   <  2:181207/151‑1 (MQ=255)

             cGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGt                                                                                                                                >  1:302391/1‑151 (MQ=255)

                  cAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATg                                                                                                                           <  2:81337/151‑1 (MQ=255)

                                cgcgcCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTgg                                                                                                             >  1:77311/1‑151 (MQ=255)

                                      ttCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTga                                                                                                        >  2:24696/1‑150 (MQ=255)

                                                  cGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCg                                                                                           <  1:150421/151‑1 (MQ=255)

                                                   gATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGt                                                                                          >  2:200777/1‑151 (MQ=255)

                                                     tGCACTCTCGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCTAAGATGTCGATGCCGCCTACGCCTGGAGTGTAGAAAACGCAc                                                                                             <  2:515081/146‑1 (MQ=255)

                                                           cACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCg                                                                                   <  2:612726/150‑1 (MQ=255)

                                                                  cATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTc                                                                           <  2:96870/151‑1 (MQ=255)

                                                                    tGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTc                                                                           >  1:399915/1‑149 (MQ=255)

                                                                        gaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGatta                                                                     <  1:267592/151‑1 (MQ=255)

                                                                         aagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGattat                                                                    >  1:515410/1‑151 (MQ=255)

                                                                             ggTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTc                                                                  >  2:298099/1‑149 (MQ=255)

                                                                               tattatCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGc                                                               >  1:583293/1‑150 (MQ=255)

                                                                                attatCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCa                                                              <  1:79888/150‑1 (MQ=255)

                                                                                attatCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCa                                                              >  2:240237/1‑150 (MQ=255)

                                                                                   atCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACAt                                                          >  2:281264/1‑151 (MQ=255)

                                                                                   atCGAATATCAGCTTTACGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGACTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACAt                                                          <  2:431031/151‑1 (MQ=255)

                                                                                     cGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACAtt                                                         <  1:22634/150‑1 (MQ=255)

                                                                                        atatCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATcg                                                     <  1:244116/151‑1 (MQ=255)

                                                                                           tCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCgg                                                  >  1:211120/1‑151 (MQ=255)

                                                                                            cAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGt                                                 >  1:331342/1‑151 (MQ=255)

                                                                                                 ttATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGtgatg                                             <  1:73727/150‑1 (MQ=255)

                                                                                                 ttATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGtgatg                                             <  1:266017/150‑1 (MQ=255)

                                                                                                  tATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGtgatga                                            <  2:576757/150‑1 (MQ=255)

                                                                                                  tATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGtgatga                                            <  2:241199/150‑1 (MQ=255)

                                                                                                   aTGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATc                                          <  1:139216/151‑1 (MQ=255)

                                                                                                         gCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTg                                     >  1:553635/1‑150 (MQ=255)

                                                                                                             ttCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGaaaa                                 >  1:381181/1‑150 (MQ=255)

                                                                                                                  gCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGaaaa                           <  1:525023/151‑1 (MQ=255)

                                                                                                                   cccGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAAt                          <  1:616450/151‑1 (MQ=255)

                                                                                                                           tACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCa                     <  1:273393/148‑1 (MQ=255)

                                                                                                                                  cGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCt           >  1:3191/1‑151 (MQ=255)

                                                                                                                                           cGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCttt  <  1:503669/151‑1 (MQ=255)

                                                                                                                                           cGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCttt  <  1:103506/151‑1 (MQ=255)

ACGCTGCTCGACACGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTT  >  NC_000913/2803691‑2803980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A