Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,106,035	C→T	P108S (CCA→TCA)	nupG →	nucleoside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,106,035	0	C	T	100.0%	158.3 / NA	43	P108S (CCA→TCA)	nupG	nucleoside transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/26); total (17/26)

GGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCG  >  NC_000913/3105887‑3106179

ggggATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCa                                                                                                                                                <  1:312922/151‑1 (MQ=255)

 gggATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTCATTAACTCGTTTGCTTATATGTCaa                                                                                                                                               <  1:164798/151‑1 (MQ=255)

    aTTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGc                                                                                                                                            >  2:534086/1‑151 (MQ=255)

       gtgGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTg                                                                                                                                         <  1:292046/151‑1 (MQ=255)

       gtgGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCAAAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTg                                                                                                                                         >  2:414457/1‑151 (MQ=255)

        tgGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTgg                                                                                                                                        >  1:167647/1‑151 (MQ=255)

           ccGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGtt                                                                                                                                     <  2:514924/151‑1 (MQ=255)

               cAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACTCCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTaac                                                                                                                                  <  2:613795/150‑2 (MQ=255)

               cAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATc                                                                                                                                 >  1:546952/1‑151 (MQ=255)

                 aaTGGTTAAGCGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCa                                                                                                                                >  1:498022/1‑150 (MQ=255)

                        aaGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCAt                                                                                                                         <  2:199/150‑1 (MQ=255)

                        aaGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCAt                                                                                                                         <  1:402872/150‑1 (MQ=255)

                         aGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCAtct                                                                                                                       <  2:427283/151‑1 (MQ=255)

                               aaaTGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACt                                                                                                                 >  1:571910/1‑151 (MQ=255)

                                aaTGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTa                                                                                                                <  2:222532/151‑1 (MQ=255)

                                 aTGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTAt                                                                                                               >  2:188563/1‑151 (MQ=255)

                                 aTGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTAt                                                                                                               <  1:287654/151‑1 (MQ=255)

                                   gggTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATc                                                                                                              <  2:288331/150‑1 (MQ=255)

                                   gggTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATc                                                                                                              <  2:625359/150‑1 (MQ=255)

                                       atatGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCt                                                                                                          <  2:322339/150‑1 (MQ=255)

                                       atatGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTg                                                                                                         >  1:6681/1‑151 (MQ=255)

                                           gCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCaa                                                                                                      >  2:38944/1‑150 (MQ=255)

                                             cATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAAt                                                                                                   <  1:224822/151‑1 (MQ=255)

                                             cATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAAt                                                                                                   >  1:553572/1‑151 (MQ=255)

                                                     acacCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGggat                                                                                           <  1:444247/151‑1 (MQ=255)

                                                       acCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGggatgg                                                                                         <  2:300098/151‑1 (MQ=255)

                                                             ggCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATc                                                                                    <  1:458947/150‑1 (MQ=255)

                                                              gcgcTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGata                                                                                      <  1:86673/147‑1 (MQ=255)

                                                               cgcTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGtt                                                                                 >  2:361933/1‑151 (MQ=255)

                                                                gcTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTa                                                                                <  1:621917/151‑1 (MQ=255)

                                                                gcTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTa                                                                                <  1:348615/151‑1 (MQ=255)

                                                                               aTGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCaa                                                                 <  1:405046/151‑1 (MQ=255)

                                                                                                   acCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATc                                             >  2:211275/1‑151 (MQ=255)

                                                                                                          gCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCttt                                       >  2:212823/1‑150 (MQ=255)

                                                                                                                ttCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATg                                 <  1:155279/150‑1 (MQ=255)

                                                                                                                  ccTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCa                              <  2:222866/151‑1 (MQ=255)

                                                                                                                       tgATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAAt                            <  1:123862/148‑1 (MQ=255)

                                                                                                                           atTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACCCCATCTCTTACTATCTCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCAGCGGCTTTATCATGGCAATgtgggtg                     <  1:521273/151‑1 (MQ=255)

                                                                                                                           atTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATgtgggt                      >  1:368374/1‑150 (MQ=255)

                                                                                                                             tGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGggtggt                   >  2:610950/1‑151 (MQ=255)

                                                                                                                                      cGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTg            <  2:236201/149‑1 (MQ=255)

                                                                                                                                            cTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCtt    >  2:523755/1‑151 (MQ=255)

                                                                                                                                              tataTGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCg  >  1:328490/1‑151 (MQ=255)

GGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCG  >  NC_000913/3105887‑3106179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A