Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F50 I1 R1
|
170 |
39.5 |
1266514 |
99.2% |
1256381 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,272,271 |
Δ1 bp |
coding (481/885 nt) |
garR ← |
tartronate semialdehyde reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,272,268 | 0 | C | . | 97.1%
| 159.8
/ ‑1.6
| 35 | coding (484/885 nt) | garR | tartronate semialdehyde reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (16/18); total (16/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01 |
CAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCAT > NC_000913/3272125‑3272409
|
cAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTAt < 2:360336/151‑1 (MQ=255)
tCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGc > 1:118737/1‑150 (MQ=255)
aCCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGcacca < 2:460653/151‑1 (MQ=255)
gAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGaa < 1:457515/151‑1 (MQ=255)
gATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCa < 2:260948/149‑1 (MQ=255)
gATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCa < 2:370045/149‑1 (MQ=255)
cAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTtca < 2:391408/151‑1 (MQ=255)
ccGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCaa < 2:554670/150‑1 (MQ=255)
gTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCAt > 2:546610/1‑151 (MQ=255)
tAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCAt > 2:390209/1‑149 (MQ=255)
aaCGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGt < 1:146676/151‑1 (MQ=255)
gCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTAtt < 2:505691/151‑1 (MQ=255)
tAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaa < 1:73219/150‑1 (MQ=255)
aGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaaa > 2:443101/1‑150 (MQ=255)
tGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAAt < 2:473425/150‑1 (MQ=255)
tGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATa < 2:242911/150‑1 (MQ=255)
gCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGc < 2:599489/151‑1 (MQ=255)
gCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTg > 2:362003/1‑151 (MQ=255)
cGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGt < 1:221193/151‑1 (MQ=255)
tCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCcca < 2:136251/150‑1 (MQ=255)
tGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCccacc > 2:309067/1‑150 (MQ=255)
gcgGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTg > 2:299968/1‑150 (MQ=255)
ttCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTAcc > 1:473686/1‑151 (MQ=255)
gcgcCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCg < 2:108680/151‑1 (MQ=255)
cacaATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATgg > 1:112123/1‑151 (MQ=255)
cacaATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATgg > 2:59007/1‑151 (MQ=255)
acaATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGc > 1:270012/1‑151 (MQ=255)
caATGACCTGATTTGCCAGTTGGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCt < 2:59140/151‑1 (MQ=255)
gACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCg > 1:456547/1‑151 (MQ=255)
tGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCAccgc > 2:528868/1‑151 (MQ=255)
ttGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCAc > 1:91471/1‑151 (MQ=255)
aCCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATcc < 2:112123/150‑1 (MQ=255)
ccTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATcc > 1:264098/1‑149 (MQ=255)
ccTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCa < 1:270259/150‑1 (MQ=255)
gCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCat > 1:206972/1‑151 (MQ=255)
|
CAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCAT > NC_000913/3272125‑3272409
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
