Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F50 I1 R1
|
170 |
39.5 |
1266514 |
99.2% |
1256381 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,264,246 |
G→A |
intergenic (‑15/‑68) |
qorA ← / → dnaB |
quinone oxidoreductase, NADPH‑dependent/replicative DNA helicase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,264,246 | 0 | G | A | 100.0%
| 100.7
/ NA
| 26 | intergenic (‑15/‑68) | qorA/dnaB | quinone oxidoreductase, NADPH‑dependent/replicative DNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/14); total (12/14) |
TCGATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAG > NC_000913/4264101‑4264389
|
tCGATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCg > 1:116743/1‑151 (MQ=255)
aTAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCa < 2:439975/151‑1 (MQ=255)
gATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCt > 2:569893/1‑149 (MQ=255)
gATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGc > 1:282231/1‑151 (MQ=255)
gCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTc < 2:56480/151‑1 (MQ=255)
cGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCtt > 2:486251/1‑148 (MQ=255)
gATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCat > 2:549326/1‑150 (MQ=255)
aTTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGt < 2:24759/151‑1 (MQ=255)
tttCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGttt > 2:483489/1‑151 (MQ=255)
cGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCg > 1:98529/1‑150 (MQ=255)
ggATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCa < 1:18002/149‑1 (MQ=255)
ggATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCAtt < 1:57704/151‑1 (MQ=255)
cATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCttt < 2:368546/149‑1 (MQ=255)
cgccgGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTa < 2:79276/151‑1 (MQ=255)
gATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTcc < 1:537345/151‑1 (MQ=255)
cGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCAt < 2:477619/150‑1 (MQ=255)
aGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACt > 1:353780/1‑151 (MQ=255)
gAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCtt > 1:616645/1‑151 (MQ=255)
ccGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCaacaaaca < 1:448932/151‑1 (MQ=255)
tgtgAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAAccc < 2:332444/149‑1 (MQ=255)
caattcGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAAcgcg < 2:623129/150‑1 (MQ=255)
cATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGcc > 1:427016/1‑151 (MQ=255)
aTGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCg < 2:478966/151‑1 (MQ=255)
gtCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCt > 2:462235/1‑151 (MQ=255)
cAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGaaa < 2:616026/149‑1 (MQ=255)
cAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAg > 2:217895/1‑150 (MQ=255)
|
TCGATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAG > NC_000913/4264101‑4264389
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
