Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,615,100	A→G	C148C (TGT→TGC)	yjjW ←	putative pyruvate formate lyase activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,615,100	0	A	G	100.0%	107.0 / NA	32	C148C (TGT→TGC)	yjjW	putative pyruvate formate lyase activating enzyme
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/14); total (18/14)

CTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTC  >  NC_000913/4614954‑4615246

ctgcGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACcaggcagg                                                                                                                                                >  2:413429/1‑151 (MQ=255)

           ctgctgATTATCGCGTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGgcgc                                                                                                                                     >  2:627854/1‑151 (MQ=255)

                       gcgTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCg                                                                                                                         >  2:216539/1‑151 (MQ=255)

                         gTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGtt                                                                                                                       >  1:122910/1‑151 (MQ=255)

                         gTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGtt                                                                                                                       >  1:575804/1‑151 (MQ=255)

                          tCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATTGCGCAGTTGCGGATCGt                                                                                                                        <  1:142838/149‑1 (MQ=255)

                          tCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGttt                                                                                                                      >  2:71464/1‑151 (MQ=255)

                          tCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGttt                                                                                                                      >  1:236478/1‑151 (MQ=255)

                           ccGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGttt                                                                                                                      <  2:476039/150‑1 (MQ=255)

                                     gttgATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGt                                                                                                           >  1:271422/1‑151 (MQ=255)

                                        gatgaCATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTaa                                                                                                         <  2:122986/150‑1 (MQ=255)

                                            aCATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAgc                                                                                                    <  1:627902/151‑1 (MQ=255)

                                             cATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAgcg                                                                                                   <  2:221623/151‑1 (MQ=255)

                                                   cTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGccacc                                                                                              >  1:338354/1‑150 (MQ=255)

                                                   cTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGccacc                                                                                              >  2:143995/1‑150 (MQ=255)

                                                    tCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCcaccac                                                                                            >  1:139209/1‑151 (MQ=255)

                                                    tCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCcaccac                                                                                            >  1:582959/1‑151 (MQ=255)

                                                             cTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGGGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACgg                                                                                      >  2:240859/1‑148 (MQ=255)

                                                                      cGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGt                                                                             <  1:413436/148‑1 (MQ=255)

                                                                      cGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCg                                                                           <  1:576624/150‑1 (MQ=255)

                                                                           aTTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCtt                                                                     <  2:376734/151‑1 (MQ=255)

                                                                             tGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTc                                                                    >  1:594892/1‑150 (MQ=255)

                                                                               cgcCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCAcc                                                                 <  2:566770/151‑1 (MQ=255)

                                                                                                 gCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATccc                                               >  1:180712/1‑151 (MQ=255)

                                                                                                       ttccccAGCCGTTTTCGCTCAACATGCCGTTACTGTCCCCCAGGCAGGTGAGAGGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGa                                          <  1:309104/147‑1 (MQ=255)

                                                                                                       ttccccAGCCGGTTTCGCTAAACATGCCGTTACTGTCCCCCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGa                                          <  1:298553/147‑1 (MQ=255)

                                                                                                                   tttCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGc                             >  2:618076/1‑151 (MQ=255)

                                                                                                                   tttCCCTCAACATGCCGTTACTGTCCCCCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGAAAGCATTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTg                              <  1:35250/150‑1 (MQ=255)

                                                                                                                                gCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATgg                >  1:596860/1‑151 (MQ=255)

                                                                                                                                    ttACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTa             >  2:90420/1‑150 (MQ=255)

                                                                                                                                         gTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACt       >  2:250089/1‑151 (MQ=255)

                                                                                                                                           ccaccaGGCAGGTGAGTTGGCGCATTTGCGGATCGTTTTTGATAGCATTAAACACCGCCCCCACAAGCGGCGGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTc     <  2:161319/151‑1 (MQ=255)

                                                                                                                                              ccaGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCCCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTc  <  2:594877/151‑1 (MQ=255)

CTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTC  >  NC_000913/4614954‑4615246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A