Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I2 R1	139	64.5	2139856	92.7%	1983646	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,737,713	(G)_6→7	intergenic (+67/‑32)	pheL → / → pheA	pheA gene leader peptide/chorismate mutase and prephenate dehydratase, P‑protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,737,707	1	.	G	91.9%	245.6 / 16.5	75	intergenic (+61/‑38)	pheL/pheA	pheA gene leader peptide/chorismate mutase and prephenate dehydratase, P‑protein
Reads supporting (aligned to +/- strand): ref base . (3/3); new base G (34/34); total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑GG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAGG  >  NC_000913/2737564‑2737847

cGGCTACTATACTAAAGTCACTTAAGCAAACAAACATGAAACACATACCGGTTTTCTTAGCATACGTTTTTAACTGCCCCTGAATCGGAGTCAGTTCGTCGTGTGAGACAGAATGCGAAGACGAACAATAATGCCGCCCAGAAC‑gg‑gggg                                                                                                                                        <  2:183757/150‑1 (MQ=255)

     actgtactAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑GG‑GGGGGCcttt                                                                                                                                  >  2:759490/1‑151 (MQ=255)

        gtactAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑GG‑GGGGGCcttttt                                                                                                                                <  1:871491/150‑1 (MQ=255)

          actAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTa                                                                                                                               <  1:455782/150‑1 (MQ=255)

             aaaGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGTCGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTg                                                                                                                            >  1:724660/1‑150 (MQ=255)

             aaaGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTg                                                                                                                            >  2:1054334/1‑150 (MQ=255)

             aaaGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGa                                                                                                                           <  2:140177/151‑1 (MQ=255)

                  cACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACa                                                                                                                      <  1:313475/151‑1 (MQ=255)

                   aCTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACaa                                                                                                                     <  2:11751/151‑1 (MQ=255)

                    cTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACaa                                                                                                                     >  2:384372/1‑150 (MQ=255)

                       aaGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACaaaaa                                                                                                                  <  1:242263/150‑1 (MQ=255)

                         ggAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGc                                                                                                               <  2:41613/151‑1 (MQ=255)

                             acaaacaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAac                                                                                                            >  2:366556/1‑150 (MQ=255)

                               aaacaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACt                                                                                                         >  1:45191/1‑151 (MQ=255)

                                aacaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTa                                                                                                        <  1:275549/151‑1 (MQ=255)

                                 acaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTAt                                                                                                       >  1:590806/1‑151 (MQ=255)

                                 acaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTAt                                                                                                       <  2:1013710/151‑1 (MQ=255)

                                 acaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑GG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATg                                                                                                      <  1:803958/151‑1 (MQ=255)

                                     gAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGAc                                                                                                    <  1:289560/150‑1 (MQ=255)

                                     gAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGAc                                                                                                    <  1:55003/150‑1 (MQ=255)

                                         cacaTACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCgg                                                                                               <  1:1054389/151‑1 (MQ=255)

                                          acaTACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGa                                                                                              <  2:479269/151‑1 (MQ=255)

                                          acaTACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGa                                                                                              <  2:387672/151‑1 (MQ=255)

                                                cGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAAccc                                                                                        <  1:427457/151‑1 (MQ=255)

                                                     ttcttcGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTa                                                                                    <  2:573742/150‑1 (MQ=255)

                                                      tcttcGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACt                                                                                  >  1:543913/1‑151 (MQ=255)

                                                         tcGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGgc                                                                               >  2:899154/1‑151 (MQ=255)

                                                          cGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGgcg                                                                              >  2:983422/1‑151 (MQ=255)

                                                            cATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGgcgc                                                                             >  2:809064/1‑150 (MQ=255)

                                                             aTTCTTTTTTACCTTCCCGTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTg                                                                           <  1:42129/151‑1 (MQ=255)

                                                             aTTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTg                                                                           <  1:708355/151‑1 (MQ=255)

                                                              ttCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGc                                                                          >  2:807643/1‑151 (MQ=255)

                                                                cTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGc                                                                          >  2:798105/1‑149 (MQ=255)

                                                                cTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCg                                                                         >  2:455999/1‑150 (MQ=255)

                                                                  tttttACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCg                                                                         >  1:295315/1‑148 (MQ=255)

                                                                   ttttACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCgag                                                                       >  1:268997/1‑149 (MQ=255)

                                                                   ttttACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCgag                                                                       >  2:179305/1‑149 (MQ=255)

                                                                           tCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCa                                                              <  1:1060131/150‑1 (MQ=255)

                                                                             cccTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcg                                                           >  2:309894/1‑151 (MQ=255)

                                                                              ccTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcgc                                                          <  1:288069/151‑1 (MQ=255)

                                                                               cTGATTGGGTGGCGTTTCGTCGTTTGATACAGAATTCGAAGGCGAACAATGAGGACTCCCAAATC‑GGTGGGGCCTTTTTTATTGATAACAAAAATCAAACACTATGACAGGTCAAAACCCGTTACTGACGCCGCGAGAGAAAATCAgcgc                                                          <  2:221514/150‑1 (MQ=255)

                                                                               cTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcgcg                                                         >  2:333641/1‑151 (MQ=255)

                                                                                tGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcgcgc                                                        >  2:141639/1‑151 (MQ=255)

                                                                                 gAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCt                                                       <  1:84233/151‑1 (MQ=255)

                                                                                 gAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑GG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTg                                                      >  1:945766/1‑151 (MQ=255)

                                                                                  aaTGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTg                                                      >  1:673875/1‑151 (MQ=255)

                                                                                     gggAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGa                                                    <  1:992093/150‑1 (MQ=255)

                                                                                       gAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGa                                                 <  2:956847/151‑1 (MQ=255)

                                                                                        aGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGaa                                                <  1:800092/151‑1 (MQ=255)

                                                                                            gTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCTGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAat                                            >  1:1018866/1‑151 (MQ=255)

                                                                                            gTTGCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGAAACACTATGACAGCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGaaaaa                                             <  2:706586/150‑1 (MQ=255)

                                                                                               tcgtcgTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑GG‑GGGGCCATTTTTATTGAGAAAAAAAAGGGAAAAATACGGCATTGGGAAAACCGGTACTGGCCACGCAAGGAAAAATCCACGGCGAGGATGAAAAattatt                                        >  2:121807/1‑151 (MQ=255)

                                                                                                 gtcgtGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAattatt                                        >  1:172509/1‑150 (MQ=255)

                                                                                                   cgtGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGc                                     <  1:376086/151‑1 (MQ=255)

                                                                                                   cgtGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGc                                     >  1:726292/1‑151 (MQ=255)

                                                                                                    gtggGAAACCGAAAGCGAAGACGAACAATAACCCATCCCAAATCGGG‑GGGGCCTATTTTATTGATAACACAAAGGCAACACAATGAAAGCGGAAAACCCGTTACTGGCCCTGCGATATAAAATCAGGGCGCCGGATGACAAAttatta                                       <  2:435256/148‑1 (MQ=255)

                                                                                                      gtgAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGtt                                  <  2:437746/151‑1 (MQ=255)

                                                                                                      gtgAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGtt                                  >  2:75057/1‑151 (MQ=255)

                                                                                                      gtgAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGt                                   <  1:886097/150‑1 (MQ=255)

                                                                                                        gAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTa                                 <  1:752077/150‑1 (MQ=255)

                                                                                                         aaaCAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTAc                                >  2:213827/1‑150 (MQ=255)

                                                                                                          aaCAGAATGCGAAGACGAACAATACGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAACGGCAACACTATGACATAGGAAAACCCGGTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTg                              >  2:1042962/1‑151 (MQ=255)

                                                                                                           aCAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTgg                             >  2:686363/1‑151 (MQ=255)

                                                                                                            cAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGc                            >  2:222128/1‑151 (MQ=255)

                                                                                                                    gAAGACGAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGgc                    >  1:141865/1‑151 (MQ=255)

                                                                                                                          gAACAATAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAAc              >  2:619009/1‑151 (MQ=255)

                                                                                                                              aaTAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGc          >  2:465623/1‑151 (MQ=255)

                                                                                                                               aTAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGcc         <  2:742866/151‑1 (MQ=255)

                                                                                                                               aTAAGGCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGcc         >  2:905653/1‑151 (MQ=255)

                                                                                                                                   ggCCTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCg     <  1:626978/151‑1 (MQ=255)

                                                                                                                                      cTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGa                         >  2:258221/1‑128 (MQ=255)

                                                                                                                                      cTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGa                         <  1:258245/128‑1 (MQ=255)

                                                                                                                                      cTCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAgg  >  1:592507/1‑151 (MQ=255)

                                                                                                                                       tCCCAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAgg  >  2:317765/1‑150 (MQ=255)

                                                                                                                                        cccAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAgg  <  2:614318/149‑1 (MQ=255)

                                                                                                                                        cccAAATCGGG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAgg  <  1:958085/149‑1 (MQ=255)

CGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATC‑GG‑GGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAGG  >  NC_000913/2737564‑2737847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A