Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,747,020	G→A	A259V (GCG→GTG)	ffh ←	Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,747,020	0	G	A	97.4%	118.6 / ‑4.2	39	A259V (GCG→GTG)	ffh	Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (16/21); total (16/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

ATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCGAC  >  NC_000913/2746878‑2747166

atCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCTCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGcaccgcaccg                                                                                                                                            >  2:521541/1‑151 (MQ=255)

atCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCAcc                                                                                                                                             <  2:452897/150‑1 (MQ=255)

     cGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGATCGGCTCCAGCGCTTCTGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTGGCCAGTGATGTGACGAATAGAGAGCACCGCAccgccg                                                                                                                                         <  1:117390/149‑1 (MQ=255)

     cGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACcgccgc                                                                                                                                        <  2:328663/150‑1 (MQ=255)

         cAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGc                                                                                                                                    >  2:564297/1‑150 (MQ=255)

                aGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGt                                                                                                                            >  2:203808/1‑151 (MQ=255)

                aGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGt                                                                                                                            >  2:319618/1‑151 (MQ=255)

                             tGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCa                                                                                                                >  1:320270/1‑150 (MQ=255)

                                       acgcgacgcgATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCg                                                                                                     <  2:100645/151‑1 (MQ=255)

                                        cgcgacgcgaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCgg                                                                                                    <  2:507473/151‑1 (MQ=255)

                                          cgacgcgaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGt                                                                                                   >  2:170509/1‑150 (MQ=255)

                                           gacgcgaTGCGGTCCGGATGGAACGGCTCCAGCTCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCTCTTTGGTCAACACTACGCCGGTa                                                                                                  <  1:678873/150‑1 (MQ=255)

                                             cgcgaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAg                                                                                                <  1:338005/150‑1 (MQ=255)

                                                          ggATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGATGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCTCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCtt                                                                                     <  1:321930/148‑1 (MQ=255)

                                                              ggAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGaa                                                                              <  2:559276/151‑1 (MQ=255)

                                                               gAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAAt                                                                             <  1:235868/151‑1 (MQ=255)

                                                                aaCGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCCCCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATg                                                                            <  1:563096/151‑1 (MQ=255)

                                                                     cTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCtt                                                                         <  1:649797/149‑1 (MQ=255)

                                                                         aGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCg                                                                   <  2:487185/151‑1 (MQ=255)

                                                                           cgcCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTa                                                                 <  2:596892/151‑1 (MQ=255)

                                                                           cgcCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGAGCCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCAGt                                                                  <  1:436798/150‑1 (MQ=255)

                                                                                cAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTg                                                              <  2:165888/149‑1 (MQ=255)

                                                                                      tctcGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCAt                                                       <  2:338721/150‑1 (MQ=255)

                                                                                       ctcGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATcc                                                     >  1:153604/1‑151 (MQ=255)

                                                                                         cgcaaccacCGAGAAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGCGCCCCGCACCGCCGCGGGCATCTCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTg                                                   <  1:70797/145‑1 (MQ=255)

                                                                                         cGCCAACACCGAGGACCTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCt                                                    <  2:186390/150‑1 (MQ=255)

                                                                                         cGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCt                                                    <  1:640686/150‑1 (MQ=255)

                                                                                                 ccGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTc                                            >  2:462200/1‑150 (MQ=255)

                                                                                                      gAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCg                                      >  1:605697/1‑151 (MQ=255)

                                                                                                      gAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCg                                      >  2:48653/1‑151 (MQ=255)

                                                                                                        aCTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTc                                    >  1:122086/1‑151 (MQ=255)

                                                                                                            gATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGTATCCTGACCGGTCATGGCGTCa                                   <  1:122995/148‑1 (MQ=255)

                                                                                                                ggTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACaa                            <  1:672545/151‑1 (MQ=255)

                                                                                                                    tGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAAc                          >  2:131294/1‑149 (MQ=255)

                                                                                                                           gATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTc                  <  1:126663/150‑1 (MQ=255)

                                                                                                                            aTGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCa                 >  2:588608/1‑150 (MQ=255)

                                                                                                                             tgtgACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAAc               >  1:77308/1‑151 (MQ=255)

                                                                                                                                  cGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGt          >  1:491336/1‑151 (MQ=255)

                                                                                                                                       agagagCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATc     >  2:651834/1‑151 (MQ=255)

                                                                                                                                          gagCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCGAc  >  2:352751/1‑151 (MQ=255)

ATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCGAC  >  NC_000913/2746878‑2747166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A