Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,747,020	G→A	A259V (GCG→GTG)	ffh ←	Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,747,020	0	G	A	93.5%	152.7 / NA	46	A259V (GCG→GTG)	ffh	Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (26/17); minor base C (0/3); total (26/20)
Fisher's exact test for biased strand distribution p-value = 7.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCG  >  NC_000913/2746878‑2747164

atCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGATTGCCAGTGATGTGACGAATAGAGAGcaccgcaccg                                                                                                                                          >  1:470121/1‑151 (MQ=255)

      gATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCgg                                                                                                                                    >  1:451659/1‑151 (MQ=255)

       aTCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCggg                                                                                                                                   <  2:447891/151‑1 (MQ=255)

        tCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGc                                                                                                                                  >  2:602140/1‑151 (MQ=255)

             gACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGc                                                                                                                             >  1:342097/1‑151 (MQ=255)

                     gTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACt                                                                                                                     >  2:72198/1‑151 (MQ=255)

                       cGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACttt                                                                                                                   <  1:381439/151‑1 (MQ=255)

                        gCCCATGCCGGGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTg                                                                                                                  >  1:35199/1‑151 (MQ=255)

                          ccATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGt                                                                                                                >  2:613151/1‑151 (MQ=255)

                              gCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAac                                                                                                            >  2:538135/1‑151 (MQ=255)

                               ccGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAac                                                                                                            >  2:42858/1‑150 (MQ=255)

                                 gagaATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACt                                                                                                         >  2:306405/1‑151 (MQ=255)

                                                 aggCGGACCGGATGGTACGGCTCCCGCGCCTCAGCTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTCATGTGACGAATAGAGAGCACAGCACCGCCGCGGGCATCTTCGGCCACTTTGGTCAACACTACGCCGGTGCGCGGt                                                                                          >  2:310327/3‑150 (MQ=255)

                                                  tGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACCCCGAGGAACTTGATGGGTTTGCCATTGATGTGACGAATAGAGAGCCCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTa                                                                                         <  2:493703/150‑1 (MQ=255)

                                                      gTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGAGGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGc                                                                                     <  1:538416/150‑1 (MQ=255)

                                                        ccGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCtt                                                                                   <  2:582123/150‑1 (MQ=255)

                                                        ccGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCtt                                                                                   <  1:291585/150‑1 (MQ=255)

                                                        ccGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGAGATGTGACGAATAGAGCGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAAAACTACGCCGGTAAGCGGTAACGCTTc                                                                                  >  1:408356/1‑151 (MQ=255)

                                                         cGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCa                                                                                 <  2:212013/151‑1 (MQ=255)

                                                         cGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCa                                                                                 <  1:271869/151‑1 (MQ=255)

                                                                 aCGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGc                                                                         >  2:593945/1‑151 (MQ=255)

                                                                   ggCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGAACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCtt                                                                       <  1:406669/151‑1 (MQ=255)

                                                                       ccAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGc                                                                   <  1:604529/151‑1 (MQ=255)

                                                                         aGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCg                                                                 >  1:459906/1‑151 (MQ=255)

                                                                           cgcCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGt                                                                >  2:276178/1‑150 (MQ=255)

                                                                              cTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTg                                                            >  2:27109/1‑151 (MQ=255)

                                                                               tCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTgg                                                           >  1:360912/1‑151 (MQ=255)

                                                                                 aGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGcc                                                         <  2:212042/151‑1 (MQ=255)

                                                                                         cGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTg                                                 <  2:662320/151‑1 (MQ=255)

                                                                                          gCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATc                                                    <  2:464296/147‑1 (MQ=255)

                                                                                            cAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGAc                                               >  2:122488/1‑150 (MQ=255)

                                                                                              acacCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCgg                                            >  2:143887/1‑151 (MQ=255)

                                                                                                 ccGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTc                                          >  1:558484/1‑150 (MQ=255)

                                                                                                  cGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCAt                                        >  1:390934/1‑151 (MQ=255)

                                                                                                   gAGGAACTTGATCGGTTTGCCAGTGATGTGGCGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAAAACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATg                                       >  2:520356/1‑151 (MQ=255)

                                                                                                    aGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATgg                                      <  2:573573/151‑1 (MQ=255)

                                                                                                              tCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACcac                            >  1:438124/1‑151 (MQ=255)

                                                                                                                      ccAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAggg                    <  2:37559/151‑1 (MQ=255)

                                                                                                                       cAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGt                   <  1:10043/151‑1 (MQ=255)

                                                                                                                       cAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGt                   <  1:399227/151‑1 (MQ=255)

                                                                                                                        aGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGtt                  >  2:78010/1‑151 (MQ=255)

                                                                                                                                gACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCgg          >  1:389013/1‑151 (MQ=255)

                                                                                                                                    aaTAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGtt       >  1:349545/1‑150 (MQ=255)

                                                                                                                                     aTAGAGAGCCCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTaa     <  2:96721/151‑1 (MQ=255)

                                                                                                                                       agagagCCCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATc   <  2:132023/151‑1 (MQ=255)

                                                                                                                                         agagCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCg  <  2:212517/150‑1 (MQ=255)

                                                                                                                                         agagCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCg  >  2:211600/1‑150 (MQ=255)

ATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCG  >  NC_000913/2746878‑2747164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A