Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I2 R1	139	64.5	2139856	92.7%	1983646	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,079,433	T→C	V46A (GTG→GCG)	yiiE →	CopG family putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,079,433	0	T	C	100.0%	181.5 / NA	51	V46A (GTG→GCG)	yiiE	CopG family putative transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/27); total (24/27)

GGCGATGAATACGGTTTTTCTTCATTTATCAGAAGAGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGTGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAACAGATGCCCTTATTCTGGTATTAATACAAGGCTGTT  >  NC_000913/4079293‑4079575

ggCGATGAATACGGTTTTTCTTCATTTATCAGAAGAGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCa                                                                                                                                      <  1:542895/151‑1 (MQ=255)

  cGATGAATACGGTTTTTCTTCATTTATCAGAAGAGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCaaa                                                                                                                                    <  1:995876/151‑1 (MQ=255)

   gATGAATACGGTTTTTCTTCATTTATCAGAAGAGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCaa                                                                                                                                     >  1:734783/1‑149 (MQ=255)

      gAATACGGTTTTTCTTCATTTATCAGAAGAGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAggg                                                                                                                                 >  2:487639/1‑150 (MQ=255)

      gAATACGGATTTTCTTCATTTATCAGAAGAGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAg                                                                                                                                   >  2:152033/1‑148 (MQ=255)

          aCGGTTTTTCTTCATTTATCAGAAGAGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGaggca                                                                                                                            <  1:827954/151‑1 (MQ=255)

          aCGGTTTTTCTTCATTTATCAGAAGAGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGaggc                                                                                                                             <  1:763009/150‑1 (MQ=255)

                 ttcttcATTTATCAGAAGAGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCaaa                                                                                                                      <  2:426383/150‑1 (MQ=255)

                  tcttcATTTATCAGAAGAGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCaaaa                                                                                                                     <  1:418120/150‑1 (MQ=255)

                   cttcATTTATCAGAAGAGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAgg                                                                                                                   >  1:400874/1‑151 (MQ=255)

                      cATTTATCAGAAGAGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAggggg                                                                                                                <  1:753660/151‑1 (MQ=255)

                              agaagaGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGtt                                                                                                        <  2:423157/151‑1 (MQ=255)

                                aagaGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTgg                                                                                                      >  2:771349/1‑151 (MQ=255)

                                 agagGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGCTAGTTGgt                                                                                                     >  1:209756/1‑151 (MQ=255)

                                     gCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTtg                                                                                                 >  2:1064825/1‑151 (MQ=255)

                                           aaaCGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAgggg                                                                                                                 <  2:5674/129‑1 (MQ=255)

                                           aaaCGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAgggg                                                                                                                 >  1:5674/1‑129 (MQ=255)

                                                 cTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAgtgt                                                                                      >  2:110785/1‑150 (MQ=255)

                                                    aaCAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAgg                                                                                  >  1:818510/1‑151 (MQ=255)

                                                     aCAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGa                                                                                 <  2:743315/151‑1 (MQ=255)

                                                      cAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAgg                                                                                  >  2:694344/1‑149 (MQ=255)

                                                       aaGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGc                                                                               >  1:437212/1‑151 (MQ=255)

                                                            tCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATaa                                                                           <  1:818408/150‑1 (MQ=255)

                                                            tCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATa                                                                            <  2:437186/149‑1 (MQ=255)

                                                              ggggCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAgg                                                                         >  1:574409/1‑150 (MQ=255)

                                                                ggCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAAt                                                                      <  1:951700/151‑1 (MQ=255)

                                                                ggCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAAt                                                                      <  1:681049/151‑1 (MQ=255)

                                                                 gCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATg                                                                     <  1:694379/151‑1 (MQ=255)

                                                                    ggCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGAc                                                                   <  2:553416/150‑1 (MQ=255)

                                                                     gCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACaa                                                                 <  2:762459/151‑1 (MQ=255)

                                                                               tCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTc                                                        >  2:568054/1‑150 (MQ=255)

                                                                                      cTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTg                                                 >  2:106104/1‑150 (MQ=255)

                                                                                        gCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCtt                                                  <  2:186197/147‑1 (MQ=255)

                                                                                        gCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGa                                                <  1:109557/149‑1 (MQ=255)

                                                                                           aTTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAatat                                            >  1:179049/1‑150 (MQ=255)

                                                                                           aTTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAAtata                                           <  1:949995/151‑1 (MQ=255)

                                                                                              ttACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATaaa                                         >  2:255014/1‑150 (MQ=255)

                                                                                                aCGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATaaaaa                                       <  2:962887/150‑1 (MQ=255)

                                                                                                aCGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATaaaaa                                       <  1:294964/150‑1 (MQ=255)

                                                                                                  gcgAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAAc                                     >  2:498383/1‑150 (MQ=255)

                                                                                                    gAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGCGCGTCAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGAAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAAc                                     >  2:671689/1‑148 (MQ=255)

                                                                                                                 aaTATCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAACAGATGCCCTTATTCTg                     >  1:318935/1‑151 (MQ=255)

                                                                                                                  atatCTGGAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAACAGATGCCCTTATTCTgg                    <  1:1003615/151‑1 (MQ=255)

                                                                                                                        ggAACGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAACAGATGCCCTTATTCTGGTAtt                <  2:1013887/149‑1 (MQ=255)

                                                                                                                           aCGTCAGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAACAGATGCCCTTATTCTGGTATTAATAc           >  1:85386/1‑151 (MQ=255)

                                                                                                                            cGTCAGAAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAACAGATGCCCTTATTCTGTTATTAATAc           >  2:331222/1‑150 (MQ=255)

                                                                                                                                aGCAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAACAGATGCCCTTATTCTGGTATTAATACAAGGc      <  2:897401/151‑1 (MQ=255)

                                                                                                                                  cAATTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAACAGATGCCCTTATTCTGGTATTAATACAAGGCTg    <  2:286365/151‑1 (MQ=255)

                                                                                                                                   aaTTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAACAGATGCCCTTATTCTGGTATTAATACAAGGCTGt   >  1:668858/1‑151 (MQ=255)

                                                                                                                                    aTTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAACAGATGCCCTTATTCTGGTATTAATACAAGGCTGtt  >  1:547088/1‑151 (MQ=255)

                                                                                                                                    aTTCCCGGCGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAACAGATGCCCTTATTCTGGTATTAATACAAGGCTGtt  <  1:885393/151‑1 (MQ=255)

GGCGATGAATACGGTTTTTCTTCATTTATCAGAAGAGGCAATTAAACGGCTGAACAAGCTTCGGGGCTGGCGTAAGGTTTCACGATCTGCAATTTTACGCGAAGCGGTAGAGCAATATCTGGAACGTCAGCAATTCCCGGTGCGTAAAGCAAAGGGAGGCAGGCAAAAGGGGGAGGTAGTTGGTGTTGATGATCAGTGTAAGGAGCATAAGGAATGACAAAAATTTTTCATTCTTGAATATAAAAAACAGATGCCCTTATTCTGGTATTAATACAAGGCTGTT  >  NC_000913/4079293‑4079575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A