Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,665,095 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,665,0950GC99.6% 647.5 / NA 280intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (116/162);  total (117/163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
*CP0425831,665,0960CG99.6% 651.3 / NA 280intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (116/162);  total (117/163)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,665,0970TA98.9% 653.4 / NA 280intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base A (117/158);  minor base C (0/3);  total (117/163)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

CCAGCGTACCGCGCGGTGACGATTTTCTCGTTGACTATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTGCTAACGTAAAATCCGGCACGGCTGACATACATCGCCTGTTCAACCATGCTGCTGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCGCTCACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGG  >  CP042583/1664816‑1665372
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                    
     gTACCGCGCGGTGACGATTTTCTCTTTGTGAATGGACAATAAACCAGTGTTGTGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGTAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCAtt                                                                                                                                                                                                                                                                             <  2:2045224/286‑1 (MQ=255)
        ccGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTgg                                                                                                                                                                                                                                                                       >  2:508106/1‑289 (MQ=255)
           cgcgGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCag                                                                                                                                                                                                                                                                    >  1:229205/1‑289 (MQ=255)
                tGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGc                                                                                                                                                                                                                                                               >  1:155778/1‑289 (MQ=255)
                tGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGc                                                                                                                                                                                                                                                               >  1:1000108/1‑289 (MQ=255)
                                                 cAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGc                                                                                                                                                                                                                               >  1:645254/1‑288 (MQ=255)
                                                 cAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCt                                                                                                                                                                                                                              <  1:781331/289‑1 (MQ=255)
                                                        gggTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCa                                                                                                                                                                                                                                                                               <  2:904607/233‑1 (MQ=255)
                                                         ggTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCATCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGa                                                                                                                                                                                                                      >  2:768378/1‑289 (MQ=255)
                                                            gACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATg                                                                                                                                                                                                                    <  1:677498/288‑1 (MQ=255)
                                                              cATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTTCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGGTGAGTTCGTTTTTCCAGGCGTGCTCGGCGCGaa                                                                                                                                                                                                                     >  2:1426035/1‑284 (MQ=255)
                                                                   ccGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGa                                                                                                                                                                                                            >  1:1783280/1‑289 (MQ=255)
                                                                    cGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGaa                                                                                                                                                                                                           <  1:716578/289‑1 (MQ=255)
                                                                    cGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGaa                                                                                                                                                                                                           <  1:1472732/289‑1 (MQ=255)
                                                                     gTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAAc                                                                                                                                                                                                          <  1:122123/289‑1 (MQ=255)
                                                                       aataaCGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACt                                                                                                                                                                                                         <  2:1925502/288‑1 (MQ=255)
                                                                       aataaCGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACt                                                                                                                                                                                                         <  2:1000108/288‑1 (MQ=255)
                                                                            cgcgTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGGGATGCCGTTCGAACTGGGTTa                                                                                                                                                                                                   >  2:281738/1‑289 (MQ=255)
                                                                            cgcgTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGtt                                                                                                                                                                                                    >  2:1696018/1‑288 (MQ=255)
                                                                            cgcgTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGAAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTa                                                                                                                                                                                                   <  2:667162/289‑1 (MQ=255)
                                                                            cgcgTGGGACCATTGCGCGTAAACGAGGTCGGCTGCGTCGAACCGTAAAATCCGGCACGCGAGACATACATCGCCTTTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTa                                                                                                                                                                                                   <  1:1613162/289‑1 (MQ=255)
                                                                               gTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACa                                                                                                                                                                                                 <  1:265331/288‑1 (MQ=255)
                                                                                 gggAACATTGCGCGTAAACAAGTTCGGCTGCGTCGAAACGTAGAAGCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACGTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCa                                                                                                                                                                                              <  2:2147444/288‑1 (MQ=255)
                                                                                   gAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGCTTTTCCAGGCGTGCCCGTCGCGATGCTTATGCAACTCGGTTACAGCaa                                                                                                                                                                                             >  2:1488998/1‑287 (MQ=255)
                                                                                         tGCGGGGAAACAAGGTCGGCTAGGTCGGAACGTAAAATCCGGCAGGCGGGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGGGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGAGGGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCa                                                                                                                                                                                       <  2:1979935/288‑1 (MQ=255)
                                                                                         tGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCag                                                                                                                                                                                      >  2:68624/1‑289 (MQ=255)
                                                                                         tGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGGGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGccg                                                                                                                                                                                      >  2:380941/1‑287 (MQ=255)
                                                                                              gTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCagagag                                                                                                                                                                                  <  1:508106/288‑1 (MQ=255)
                                                                                                aacacaGGTCCGCTGCGTCGAAACGTTAAACCCCGAACGCGCGACATAAATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCGGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTg                                                                                                                                                                               <  2:1320962/284‑1 (MQ=255)
                                                                                                   cAAGGTCGGCTGCGTCGAAACGGAAAAACCGGCACGCGGGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGGGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGaaa                                                                                                                                                                            <  2:2129587/289‑1 (MQ=255)
                                                                                                         cGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGGGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGTGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAGCTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGt                                                                                                                                                                      <  1:1200996/289‑1 (MQ=255)
                                                                                                                 tcgaaaCGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTAc                                                                                                                                                              >  1:1816953/5‑289 (MQ=255)
                                                                                                                 tcgaaaCGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTAc                                                                                                                                                              >  1:1966646/5‑289 (MQ=255)
                                                                                                                  cgaaaCGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACg                                                                                                                                                             <  1:842175/286‑1 (MQ=255)
                                                                                                                                   cacgcgagACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCAt                                                                                                                                              <  2:1783280/279‑1 (MQ=255)
                                                                                                                                            catccatcGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGt                                                                                                                                    <  1:1266263/287‑1 (MQ=255)
                                                                                                                                                catCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAg                                                                                                                              >  1:139922/1‑289 (MQ=255)
                                                                                                                                                  tCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGAt                                                                                                                            <  1:1121320/289‑1 (MQ=255)
                                                                                                                                                    gCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCCTGCCGCAGGGAGGTGCAAGCCAGTGGCCTTACG‑GAGGTGTCGCGTAGTCCATCACTTAAAAAgtcac                                                                                                                          >  2:54101/1‑285 (MQ=255)
                                                                                                                                                    gCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCa                                                                                                                          >  2:983812/1‑289 (MQ=255)
                                                                                                                                                     ccTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCCGTGGCGCTACGAGAGCT‑TCGCGCCTTCCATTACGGAAAAAGATCAg                                                                                                                         >  2:631968/1‑289 (MQ=255)
                                                                                                                                                     ccTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCa                                                                                                                                               <  2:1550125/267‑1 (MQ=255)
                                                                                                                                                          tcaaccatcgacgaggAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGt                                                                                                                    <  2:1889565/275‑1 (MQ=255)
                                                                                                                                                          tcaaccatcgacgaggAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGt                                                                                                                    <  1:886448/275‑1 (MQ=255)
                                                                                                                                                            aaccatcgacgaggAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGTGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGACAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCg                                                                                                                  <  1:1756103/277‑1 (MQ=255)
                                                                                                                                                             accatcgacgaggAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGa                                                                                                                 <  1:1071818/278‑1 (MQ=255)
                                                                                                                                                             accatcgacgaggAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGa                                                                                                                 <  1:1863712/278‑1 (MQ=255)
                                                                                                                                                              ccatcgacgaggAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGAc                                                                                                                >  1:1230929/11‑289 (MQ=255)
                                                                                                                                                               catcgacgaggAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACg                                                                                                               <  1:1549996/280‑1 (MQ=255)
                                                                                                                                                                atcgacgaggAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGt                                                                                                              >  1:442410/9‑289 (MQ=255)
                                                                                                                                                                   gacgaggAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACCGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGCCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCCTCACGCAGAGAGGTGAAAGCCAGTGGCGCTACGAGAGCT‑TCACGCGCGCCATTATGTAAAAAGACCAGTTTGTCTACGatat                                                                                                           >  2:568068/6‑289 (MQ=255)
                                                                                                                                                                   gacgaggAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAAc                                                                                                                                                                                                          >  2:444008/6‑195 (MQ=255)
                                                                                                                                                                   gacgaggAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGa                                                                                                                 <  2:129588/278‑1 (MQ=255)
                                                                                                                                                                   gacgaggAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGa                                                                                                                 >  1:129588/6‑283 (MQ=255)
                                                                                                                                                                   gacgaggAGTTGTGCGCTAGTCGCAGAAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGGGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTtat                                                                                                           <  1:602538/284‑1 (MQ=255)
                                                                                                                                                                     cgaggAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTtata                                                                                                          <  1:53791/285‑1 (MQ=255)
                                                                                                                                                                              gtgCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCg                                                                                                                  <  2:1227351/271‑1 (MQ=255)
                                                                                                                                                                               tgCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCa                                                                                                                                               <  1:238029/241‑1 (MQ=255)
                                                                                                                                                                                 cgcTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTa                                                                                             >  2:327170/1‑289 (MQ=255)
                                                                                                                                                                                      gTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCAtt                                                                                                                                                                                                                                                                             <  1:472821/109‑1 (MQ=255)
                                                                                                                                                                                      gTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTa                                                                                                                                                                                                   >  1:1924137/1‑183 (MQ=255)
                                                                                                                                                                                      gTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGt                                                                                                                    >  2:104650/1‑261 (MQ=255)
                                                                                                                                                                                      gTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCg                                                                                                                  <  1:1420177/263‑1 (MQ=255)
                                                                                                                                                                                      gTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCA‑TAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAgc                                                                                       >  1:1054886/1‑289 (MQ=255)
                                                                                                                                                                                        cGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAgcg                                                                                      <  1:327170/289‑1 (MQ=255)
                                                                                                                                                                                         ggaggaaatAACCACGTTCCCGTGCCGCGGTAATTGGCTTGTCGAGGATTTTATTTGCGAGGGGCATGAGATTCGCTTTGCTGACTGATGCATCCGACAAACCATTACCGGGCAGAGTGCGTCGTCGGTTGAGTGCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGAGCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAgcgc                                                                                     <  2:1163737/281‑1 (MQ=255)
                                                                                                                                                                                         gcagcaAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑Tcgcg                                                                                                                                                 >  1:586362/1‑229 (MQ=255)
                                                                                                                                                                                           agcaAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTTAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTTCAAATGCTTATctactgcgt                                                                                   >  2:658325/1‑281 (MQ=255)
                                                                                                                                                                                                 tAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCa                                                                              >  1:766223/1‑288 (MQ=255)
                                                                                                                                                                                                   aCCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACg                                                                           <  1:2134179/289‑1 (MQ=255)
                                                                                                                                                                                                       aCTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGAGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGttt                                                                        >  1:2051752/1‑288 (MQ=255)
                                                                                                                                                                                                           ccAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGACCGCGTTGGCAACGTTTTCCAg                                                                   >  2:951809/1‑289 (MQ=255)
                                                                                                                                                                                                                gCAGCGGTAATTCATTTGTCGAGTCTTTCCTTTAGCCGAGAAAGGAGATTCCCACCGCTGACTAATGCATCCGACACACCATTAACTGGCAGATGGCGTCGTCGGTTGAGTTCGATGTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAgatga                                                               <  2:2193177/288‑1 (MQ=255)
                                                                                                                                                                                                                 cAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGAtt                                                             >  1:2233665/1‑289 (MQ=255)
                                                                                                                                                                                                                    cGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTAc                                                           >  1:1997464/1‑288 (MQ=255)
                                                                                                                                                                                                                    cGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCG‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTTCAAATGCTTATCGAGCGCCTTGGCAACGTTTTCCATATGATTAc                                                           >  2:126309/1‑288 (MQ=255)
                                                                                                                                                                                                                     ggTAATTTCATTGTCGCAGCTTTCAGTTTCGCGAGACAGGAGATTCCCCACGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACg                                                          <  2:235376/288‑1 (MQ=255)
                                                                                                                                                                                                                     ggTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACg                                                          <  2:139922/288‑1 (MQ=255)
                                                                                                                                                                                                                     ggTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACg                                                          <  1:2170287/288‑1 (MQ=255)
                                                                                                                                                                                                                         attttcttGTCGATGCCTTCAGTTGCGAGGACCAGGTGGTTCCCCTTGCATACTAATGCAACCGGCAAACCCTTAACTGGCAGAGTGCGTGGTCGGTTGAGTTCGAGTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCAGCACGCAGGGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGTTCAGTTTGTCGACGTTATACTGCTAATGCTTATCGAGCGTGTTGGCAACGTTTTCCAGATTATTACgctggc                                                     <  2:1863171/283‑1 (MQ=255)
                                                                                                                                                                                                                            tCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGctggctgg                                                  <  1:983812/289‑1 (MQ=255)
                                                                                                                                                                                                                             cATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTACCTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGga                                                 <  2:1966646/289‑1 (MQ=255)
                                                                                                                                                                                                                                 gTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTg                                                                                                                                                                               <  2:1587799/160‑1 (MQ=255)
                                                                                                                                                                                                                                 gTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGata                                             >  1:1587128/1‑289 (MQ=255)
                                                                                                                                                                                                                                        cTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCa                                       <  2:424031/288‑1 (MQ=255)
                                                                                                                                                                                                                                        cTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCa                                       <  2:155778/288‑1 (MQ=255)
                                                                                                                                                                                                                                             aTTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTcc                                 <  2:742526/289‑1 (MQ=255)
                                                                                                                                                                                                                                                 tCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAAc                                                                                                                                                                                                          >  1:1786365/1‑117 (MQ=255)
                                                                                                                                                                                                                                                     gagaCAGGAGATTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGcaccac                         <  1:93120/289‑1 (MQ=255)
                                                                                                                                                                                                                                                            gagaTTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCACCGTTTTCCAGATGATTAACCTGGCTGGAGATATAGGCATATTCAAGcacacacacatc                   >  2:1638408/1‑279 (MQ=255)
                                                                                                                                                                                                                                                             ggaTTCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACtt                    <  2:1149803/285‑1 (MQ=255)
                                                                                                                                                                                                                                                               aTTCCCCACGCTGACTAATGCATACGACACACCATTAACTGGCCGAGTGCGTCGTCGTTTTGGTTCGATTTTCCAGTCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGATCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACg                <  2:1028071/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                ttCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACg                <  1:388614/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                 tCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACg                                                          >  2:1789448/1‑244 (MQ=255)
                                                                                                                                                                                                                                                                 tCCCCTCGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCGGGAGATATGGGCATCTTCCAGCACCACCACTTAACGcac             >  2:1061590/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                       cGCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAACTTCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATCTAGGCATCTTCCAGCACCACCACTTCACGCCCGGTGAg       >  2:1289253/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                        gCTGACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGctggctgg                                                  >  1:1041829/1‑245 (MQ=255)
                                                                                                                                                                                                                                                                           gACTAATGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCa                                                                    <  1:1455013/224‑1 (MQ=255)
                                                                                                                                                                                                                                                                            aCTAATGCATCCGCAACACCATTTACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATGTTCCAGGCGTGATCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAgg  <  2:2233665/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                aTGCATCCGACACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGt                                                                                                                    <  1:1684910/171‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                    
CCAGCGTACCGCGCGGTGACGATTTTCTCGTTGACTATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTGCTAACGTAAAATCCGGCACGGCTGACATACATCGCCTGTTCAACCATGCTGCTGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATCGCTCACACCATTAACTGGCAGAGTGCGTCGTCGGTTGAGTTCGATTTTCCAGGCGTGCTCGTCGCGATGCTGTTCGAACTCGGTTACAGCATCACGCAGAGAGGTGAAATCCAGTGGCGCTACGAGAGCT‑TCGCGCATGCCATTACGTAAAAAGATCAGTTTGTCGACGTTATACTGCAAATGCTTATCGAGCGCGTTGGCAACGTTTTCCAGATGATTACGCTGGCTGGAGATATAGGCATCTTCCAGCACCACCACTTCACGCCAGGTGAGCAAGG  >  CP042583/1664816‑1665372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A