Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F210 I1 R1 4928 385.2 6658600 97.5% 6492135 231.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,489,942 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,489,9420AT99.8% 955.5 / NA 401intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (210/189);  minor base C (1/0);  total (212/189)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,489,9430GC99.2% 941.0 / NA 401intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base C (209/187);  total (212/189)
Fisher's exact test for biased strand distribution p-value = 6.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,489,9440CG99.2% 934.5 / NA 401intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base G (207/187);  total (212/189)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 401 total aligned reads displayed.

GTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGAGCCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGAGTACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGA  >  CP042583/1489663‑1490216
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                 
      ggtgTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAATGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTCAAACCg                                                                                                                                                                                                                                                                      >  1:964084/1‑289 (MQ=255)
          tgCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGACCTGCTTCCCGGTTTTACCGCCTAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACAGtaa                                                                                                                                                                                                                                                                   >  2:1694596/1‑286 (MQ=255)
          tgCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCa                                                                                                                                                                                                                                                                   >  1:3192953/1‑288 (MQ=255)
          tgCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGA‑TTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAc‑c                                                                                                                                                                                                                                                                <  2:2397832/289‑1 (MQ=255)
           gCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTAGTGTCGCGTAGTAAAACCGTCAc‑c                                                                                                                                                                                                                                                                >  1:2870767/1‑289 (MQ=255)
           gCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAc‑c                                                                                                                                                                                                                                                                <  2:323014/289‑1 (MQ=255)
               tAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTg                                                                                                                                                                                                                                                             >  2:371995/1‑288 (MQ=255)
                   tCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCt                                                                                                                                                                                                                                                          <  2:276654/287‑1 (MQ=255)
                      tgtTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCCCGTAGTAAAACCGTCCCACGT‑CCTAAGGt                                                                                                                                                                                                                                                     >  2:1538107/1‑289 (MQ=255)
                                           cgcTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCg                                                                                                                                                                                                                                >  1:1652073/1‑289 (MQ=255)
                                           cgcTAACATTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCg                                                                                                                                                                                                                                >  2:1055919/1‑289 (MQ=255)
                                                         tCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGGGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGtttt                                                                                                                                                                                                                   <  1:1694596/288‑1 (MQ=255)
                                                         tCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGtttt                                                                                                                                                                                                                   <  1:1208851/288‑1 (MQ=255)
                                                            ggCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTgg                                                                                                                                                                                                               >  2:1574260/1‑289 (MQ=255)
                                                                   aacaTGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGa                                                                                                                                                                                                        >  1:2436264/1‑289 (MQ=255)
                                                                   aacaTGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGa                                                                                                                                                                                                        <  1:857904/289‑1 (MQ=255)
                                                                     caTGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGAtt                                                                                                                                                                                                      >  1:2141210/1‑289 (MQ=255)
                                                                     aaTGGGTGAGACATCATGTGGCAGGCAATCAGTCGTCTTTGGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGAtt                                                                                                                                                                                                      <  1:1605236/288‑1 (MQ=255)
                                                                           agagaCATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTa                                                                                                                                                                                                                          >  1:2776737/1‑263 (MQ=255)
                                                                                catcatGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCAc                                                                                                                                                                                             <  1:686569/287‑1 (MQ=255)
                                                                                        ggcaggcaATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGc                                                                                                                                                                                    <  2:1652073/288‑1 (MQ=255)
                                                                                        ggcaggcaATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGc                                                                                                                                                                                    <  1:772282/288‑1 (MQ=255)
                                                                                           aggcaATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGa                                                                                                                                                                                <  2:1482064/289‑1 (MQ=255)
                                                                                           aggcaATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACACGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGa                                                                                                                                                                                >  1:2589425/1‑289 (MQ=255)
                                                                                                      cgtcTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGGAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAg                                                                                                                                                                       <  1:330282/287‑1 (MQ=255)
                                                                                                                   agcAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTTCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTg                                                                                                                                                                                                             >  2:296918/1‑236 (MQ=255)
                                                                                                                   agcAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGc                                                                                                                                                                                    <  1:1141053/261‑1 (MQ=255)
                                                                                                                   agcAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTgg                                                                                                                                                        >  1:1077238/1‑289 (MQ=255)
                                                                                                                                  gCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGcgcg                                                                                                                                         >  2:127213/1‑289 (MQ=255)
                                                                                                                                  gCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGcgcg                                                                                                                                         >  1:2729810/1‑289 (MQ=255)
                                                                                                                                  gCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGCGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGAACGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTTTTTGTTAGCAATTTTcgcg                                                                                                                                         >  2:1309897/1‑289 (MQ=255)
                                                                                                                                   cGAAATCAAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGcgcg                                                                                                                                         <  1:1019665/288‑1 (MQ=255)
                                                                                                                                    gAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGtt                                                                                                                                       <  1:1502846/289‑1 (MQ=255)
                                                                                                                                             cTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGCTACTGTCGCGTAGTAAAACCGTCCC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGCTACTACCGGTTTCCGGTGATGGATTATCTCCCACCTCGTCCGCCGGACGCGCCTAGCGCATTTATTCTTGGTCAGCACATTgctgggttacttcgat                                                                                                                              >  2:1398395/1‑275 (MQ=255)
                                                                                                                                                 gTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCt                                                                                                                                                                                           >  1:1566603/1‑224 (MQ=255)
                                                                                                                                                      gAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGacc                                                                                                                     <  1:127213/289‑1 (MQ=255)
                                                                                                                                                          tGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAc                                                                                                                                                                                                                                                                  >  1:1771486/1‑145 (MQ=255)
                                                                                                                                                          tGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGc                                                                                                                                                                                    <  1:1813811/222‑1 (MQ=255)
                                                                                                                                                          tGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGc                                                                                                                                                                                    >  2:1813811/1‑222 (MQ=255)
                                                                                                                                                          tGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTa                                                                                                                                     >  2:1078793/1‑269 (MQ=255)
                                                                                                                                                          tGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGaccaac                                                                                                                  >  1:1934162/1‑288 (MQ=255)
                                                                                                                                                          tGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGaccaac                                                                                                                  >  1:396726/1‑288 (MQ=255)
                                                                                                                                                          tGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTAAAGTTTTCTGGTGa                                                                                                                                                                                                            >  2:1137875/1‑198 (MQ=255)
                                                                                                                                                            ccTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTa                                                                                                                                                                                                     <  1:111954/203‑1 (MQ=255)
                                                                                                                                                            ccTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGc                                                                                                                                                                                    >  2:3110109/1‑220 (MQ=255)
                                                                                                                                                            ccTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGACTAGCCCATTTATTCTTCGTCAGCAACTTGCGGCTTTACATCAATGGAGGCACCAATcac                                                                                                               >  2:674709/1‑289 (MQ=255)
                                                                                                                                                              tGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACaa                                                                                                             >  1:1881364/1‑289 (MQ=255)
                                                                                                                                                                    agaAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTg                                                                                                                                                                                                             >  2:3177407/1‑187 (MQ=255)
                                                                                                                                                                      aaGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCt                                                                                                                                                                                                                                                          >  1:265553/1‑140 (MQ=255)
                                                                                                                                                                      aaGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAg                                                                                                                                                                       >  2:2081208/1‑223 (MQ=255)
                                                                                                                                                                      aaGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAg                                                                                                                                                                       <  1:2081208/223‑1 (MQ=255)
                                                                                                                                                                      aaGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAg                                                                                                                                                                       <  2:858958/223‑1 (MQ=255)
                                                                                                                                                                      aaGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCt                                                                                                     >  2:2149508/1‑289 (MQ=255)
                                                                                                                                                                              gCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCTTTTACATCAATGGATCGACCAACCACAATTTGGCCTTTATTTCg                                                                                             >  2:2862810/1‑289 (MQ=255)
                                                                                                                                                                              gCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACAGCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCg                                                                                             >  1:255463/1‑289 (MQ=255)
                                                                                                                                                                                  cATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTgc                                                                                                                                            >  1:143585/1‑238 (MQ=255)
                                                                                                                                                                                  cATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGCGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCAt                                                                                                                                                                  <  2:200057/216‑1 (MQ=255)
                                                                                                                                                                                  cATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGCGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCAt                                                                                                                                                                  >  1:200057/1‑216 (MQ=255)
                                                                                                                                                                                   aTGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATaa                                                                                         >  2:1369377/1‑288 (MQ=255)
                                                                                                                                                                                   aTGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAAc                                                                                        >  2:92972/1‑289 (MQ=255)
                                                                                                                                                                                   aTGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAAc                                                                                        <  2:576930/289‑1 (MQ=255)
                                                                                                                                                                                    tGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAAcg                                                                                       <  2:193994/289‑1 (MQ=255)
                                                                                                                                                                                       cATTTGGGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAAcgcg                                                                                     <  2:2598875/288‑1 (MQ=255)
                                                                                                                                                                                       cATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAAcgcg                                                                                     <  1:1309897/288‑1 (MQ=255)
                                                                                                                                                                                               gtATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAGCTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACGGTCGCGTAGTAAAGCCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTTGATGCGCATTGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTa                                                                                                                                     <  1:1976166/231‑1 (MQ=255)
                                                                                                                                                                                                 aTGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCg                                                                                                                                                                                     >  1:2516064/1‑182 (MQ=255)
                                                                                                                                                                                                 aTGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGa                                                                                                                                                                                <  2:2179409/187‑1 (MQ=255)
                                                                                                                                                                                                   gCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAc‑c                                                                                                                                                                                                                                                                >  1:903219/1‑105 (MQ=255)
                                                                                                                                                                                                   gCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAc‑c                                                                                                                                                                                                                                                                <  2:903219/105‑1 (MQ=255)
                                                                                                                                                                                                      ggcgaTGATTTTTTCGTCAAATGGGATGACAGGGACCTGCTTCCCGGTTTTACCGCCGACGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCca                                                                      <  2:2537788/284‑1 (MQ=255)
                                                                                                                                                                                                       gACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCcaca                                                                    >  1:3093872/1‑289 (MQ=255)
                                                                                                                                                                                                       gACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGGGCCTAAGGTTTGGGCAGTTTGCGCTGCCCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGCTGCGCATAGCGCATTTCTTCTTGGTCAGCAAATTGCGCGTTTACCTCAATGGAGACACCACCCACAATTTGCCCTTGTTTTCGACACTCCGCCCTCCACCACTCcaac                                                                    >  2:33361/1‑287 (MQ=255)
                                                                                                                                                                                                        aCATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCca                                                                      <  1:1369377/286‑1 (MQ=255)
                                                                                                                                                                                                        aCATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATATCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCca                                                                      <  1:1055919/286‑1 (MQ=255)
                                                                                                                                                                                                         cATGATTTTTTCGTCAAATGTGCTGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCTCCTAGTAACACCGTCGC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTTACCGTGACTACAGCTTTCTGGTGATGGATTCTCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAACTTCCGCGTTTACATCAATGGa                                                                                                                           >  2:1976166/1‑232 (MQ=255)
                                                                                                                                                                                                           tGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGccc                                                                <  2:1213937/289‑1 (MQ=255)
                                                                                                                                                                                                            gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCTTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGccc                                                                >  1:3143585/1‑288 (MQ=255)
                                                                                                                                                                                                            gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGaccaacca                                                                                                                <  2:3033860/240‑1 (MQ=255)
                                                                                                                                                                                                            gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACCATTTTGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCa                                                               >  2:1764701/1‑289 (MQ=255)
                                                                                                                                                                                                            gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAAcgcg                                                                                     <  1:1861929/267‑1 (MQ=255)
                                                                                                                                                                                                            gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAAcgcg                                                                                     >  2:1861929/1‑267 (MQ=255)
                                                                                                                                                                                                            gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCca                                                                      <  2:1155629/282‑1 (MQ=255)
                                                                                                                                                                                                            gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCca                                                                      <  1:922046/282‑1 (MQ=255)
                                                                                                                                                                                                            gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCa                                                               >  2:2470368/1‑289 (MQ=255)
                                                                                                                                                                                                            gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCa                                                               >  2:2309256/1‑289 (MQ=255)
                                                                                                                                                                                                            gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCa                                                               >  1:3256197/1‑289 (MQ=255)
                                                                                                                                                                                                            gATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGGTTTACCGCCGAAGACGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGAGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCCCGTCCGCTGGATGCGCATAGCGCATTTATTCTTCGTCAGCAAATGACGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTTGAACACTCGCTCTCTGTCACTCCACATCCCa                                                               >  2:81873/1‑289 (MQ=255)
                                                                                                                                                                                                                      tCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCa                                                     >  1:215812/1‑289 (MQ=255)
                                                                                                                                                                                                                                       gAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTAGCAACGTCGCTggtcaacgt                                    >  1:2807256/1‑282 (MQ=255)
                                                                                                                                                                                                                                         aCTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTa                                                                                                                                     >  1:359212/1‑190 (MQ=255)
                                                                                                                                                                                                                                         aCTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTggtcaacgtt                                   <  2:2729810/288‑9 (MQ=255)
                                                                                                                                                                                                                                           tGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGttttt                                <  2:2579923/289‑1 (MQ=255)
                                                                                                                                                                                                                                            gCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGt                                                                                                                                                                                                                                            >  1:2633102/1‑84 (MQ=255)
                                                                                                                                                                                                                                             cTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCa                                                                                                                                                                          <  2:2642521/149‑1 (MQ=255)
                                                                                                                                                                                                                                             cTTCCCGGGTTTCACGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTg                              <  2:1881364/289‑1 (MQ=255)
                                                                                                                                                                                                                                                cccGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTg                           <  1:2309256/289‑1 (MQ=255)
                                                                                                                                                                                                                                                   ggTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGaac                        <  1:3024764/289‑1 (MQ=255)
                                                                                                                                                                                                                                                    gTTTTACCGCCGAGGCCGACCAACTGGAGTGACGGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGaac                        <  2:743521/288‑1 (MQ=255)
                                                                                                                                                                                                                                                        tACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTggtcaacgtt                                   <  1:2949454/273‑9 (MQ=255)
                                                                                                                                                                                                                                                                       ccAACTGGAGTTACTGTCGCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGTCAACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTg    >  1:597058/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                 
GTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGAGCCGTAGTAAAACCGTCAC‑CGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTATCTCCCACCTCGTCCGCTGGATGCGCATAGCGCATTTATTCTTGGTCAGCAAATTGCGCGTTTACATCAATGGAGCGACCAACCACAATTTGGCCTTGATTTCGATAACGCGCTCTCCACAACTCCACAGCCCAACACCTGGCAACGTCGCTGGAGTACGTTTTTTGCTGAACAACGGATTGGCTGGCAGTTGGA  >  CP042583/1489663‑1490216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A