Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,646,891 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,646,8910AT99.1% 503.9 / NA 216intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (101/111);  minor base C (1/1);  total (102/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,646,8920CG99.5% 508.3 / ‑6.1 216intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (102/113);  minor base T (0/1);  total (102/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
*CP0425831,646,8930TA99.1% 503.1 / NA 216intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (101/111);  minor base G (0/2);  total (102/114)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

GCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATAACTGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATGCTCGTCATTGGCTGCCCACTGGTCATACGACTAGCCAGAATAATTCCGGCTATCGCACTTACCAGGCCACTGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCACTAATGATGTACGCCAGACCTCGAACAATCTGCATCGTTGCCA  >  CP042583/1646611‑1647168
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                    
  ttGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACa                                                                                                                                                                                                                                                                              <  2:1704344/288‑1 (MQ=255)
  ttGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACa                                                                                                                                                                                                                                                                              <  1:453328/288‑1 (MQ=255)
        cGGTCGAAGATCACCGCTGCCAGCAAGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGataa                                                                                                                                                                                                                                                                       <  1:1591492/289‑1 (MQ=255)
                           ccAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGaacaa                                                                                                                                                                                                                                                     >  2:254007/1‑288 (MQ=255)
                                   aTTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGACTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTa                                                                                                                                                                                                                                               <  1:1841890/286‑1 (MQ=255)
                                     tAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACAccc                                                                                                                                                                                                                                          >  1:1656151/1‑289 (MQ=255)
                                     tAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACAccc                                                                                                                                                                                                                                          >  1:1217166/1‑289 (MQ=255)
                                     tAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACAcc                                                                                                                                                                                                                                           >  1:768040/1‑288 (MQ=255)
                                                      aCTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCctc                                                                                                                                                                                                                         <  1:1535899/289‑1 (MQ=255)
                                                       atgggaggaaGGAGAAATATTAAGCAGGTTCATGGCGTATGCCACGGTGCCAAAAATTAAGATACCCGCAACCACATATGAGATTTTACCGATGCCACCGTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCctct                                                                                                                                                                                                                        <  2:735495/281‑1 (MQ=255)
                                                        tGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGaac                                                                                                                                                                                                                                                       >  2:381552/1‑257 (MQ=255)
                                                        tGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGaac                                                                                                                                                                                                                                                       <  1:381552/257‑1 (MQ=255)
                                                          cgcgAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTTCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCg                                                                                                                                                                                                                     >  1:786718/1‑289 (MQ=255)
                                                          cgcgAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCg                                                                                                                                                                                                                     >  1:813251/1‑289 (MQ=255)
                                                           gcgAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAACATCATAATTTTGGTGCGAACAACAGGTACACCCGCCAGACGCGCGGCCTCTTCGt                                                                                                                                                                                                                    >  1:1455202/1‑289 (MQ=255)
                                                           gcgAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGACATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACACGCGGCCTATTCGt                                                                                                                                                                                                                    >  2:1745901/1‑289 (MQ=255)
                                                             gAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGCCGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGACCTGAGCG‑AACAACGATAATAATTTTGGTGCCAACACCCGGTACACCCGCCAGACGCGCGGCCTCTCCGtt                                                                                                                                                                                                                   >  1:1194935/1‑288 (MQ=255)
                                                               aaGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCGATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTcc                                                                                                                                                                                                                 >  1:987385/1‑288 (MQ=255)
                                                                   cgaAATCTTAAGCAGGTTCATGGCGTTTTCCACGGTTCCTAAAAGTAAGATACCCCCCACCACATATGAGATTTTTCCGATGCCACCTTTAAGAGAAACGCCACCTAAAACGCAGGCGGAGATAAAAATCCGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGcc                                                                                                                                                                                                             <  2:602678/287‑1 (MQ=255)
                                                                    gaAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCa                                                                                                                                                                                                            <  1:1928727/288‑1 (MQ=255)
                                                                        tatTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGc                                                                                                                                                                                                       <  1:1060475/289‑1 (MQ=255)
                                                                        tatTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGc                                                                                                                                                                                                       <  1:911728/289‑1 (MQ=255)
                                                                          ttAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCa                                                                                                                                                                                                     <  2:593249/289‑1 (MQ=255)
                                                                                  gTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGtt                                                                                                                                                                                              >  1:552166/1‑288 (MQ=255)
                                                                                  gTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGtt                                                                                                                                                                                              >  1:415720/1‑288 (MQ=255)
                                                                                  gTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGtt                                                                                                                                                                                              >  1:1124409/1‑288 (MQ=255)
                                                                                  gTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGACAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCGCATAACCAATCCACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGAAACCAGGCCTGAGAG‑CACAAAGATAATAATTTCGGTGCGAACAACCGGTACACCCGCCAGACGCGATGCCTCTTCGTCCACGCCAACCGCCCGGgtgct                                                                                                                                                                                              >  2:1149712/1‑286 (MQ=255)
                                                                                   ttCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTAc                                                                                                                                                                                            >  2:854525/1‑289 (MQ=255)
                                                                                    tCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACg                                                                                                                                                                                           >  1:292146/1‑289 (MQ=255)
                                                                                            ttttCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTAAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAgg                                                                                                                                                                                   <  2:462771/289‑1 (MQ=255)
                                                                                             tttCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTAACGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCACTTCGTTCCCGCCAATCGCTCGGGGGGTACGACCGAAggg                                                                                                                                                                                  >  2:521603/1‑288 (MQ=255)
                                                                                               tCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtgg                                                                                                                                                                                <  1:735861/289‑1 (MQ=255)
                                                                                                    ggTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCa                                                                                                                                                                                                            >  1:1681273/1‑256 (MQ=255)
                                                                                                    ggTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCa                                                                                                                                                                                                            <  2:1681273/256‑1 (MQ=255)
                                                                                                      tGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAg                                                                                                                                                                                                    <  1:1555581/262‑1 (MQ=255)
                                                                                                       gCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTAtt                                                                                                                                                                         <  1:955954/288‑1 (MQ=255)
                                                                                                        ccTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTc                                                                                                                                                                        >  2:32131/1‑288 (MQ=255)
                                                                                                        ccTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCCCCAATCCCCAGGGTGTTACGACCAAAGGTGGTTTTATTcc                                                                                                                                                                       >  2:1824303/1‑288 (MQ=255)
                                                                                                        ccTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGCCGGAGATAACAATCAGCTCATAACCAATCCACGCCATTGGCTGCCCACTGGTCATCCGTGAAGCCAGAATACTTCCTGCTATCGCTGATCCCAGGCCTGAGAG‑AACAAGGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCGTCGTTCCCCCCAATCGCAAGGGTGTTACGACCAAGGGTGGTTTTATGca                                                                                                                                                                       >  2:644989/1‑289 (MQ=255)
                                                                                                           aaaaTTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGATGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATACCCAGGGTGTTACGACCAAAGCTGGTCTTATTcagc                                                                                                                                                                     >  2:335147/1‑288 (MQ=255)
                                                                                                                tAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTACGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGGTTTATTAAGCAGCaaa                                                                                                                                                               >  1:1249448/1‑289 (MQ=255)
                                                                                                                  aGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaa                                                                                                                                                                                      <  1:1518420/264‑1 (MQ=255)
                                                                                                                  aGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaa                                                                                                                                                                                      >  2:1518420/1‑264 (MQ=255)
                                                                                                                  aGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAcc                                                                                                                                                             >  2:1481074/1‑289 (MQ=255)
                                                                                                                     tACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCaaa                                                                                                                                                          <  2:1567105/289‑1 (MQ=255)
                                                                                                                       cccGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAAGCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACC‑GGCCCGAAAG‑AACAAAGATAAAAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCCCCAGGGGGTTACGACCAAAGGGGGGTTTATTCACCCGCaaaaccaataa                                                                                                                                                       >  2:1657863/1‑281 (MQ=255)
                                                                                                                         cGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGACCAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTCTTCAGCAGCAAACCAACGATa                                                                                                                                                      >  1:1488610/1‑289 (MQ=255)
                                                                                                                             accacATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCa                                                                                                                                                  >  2:612701/1‑289 (MQ=255)
                                                                                                                                             ttCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGAcgcg                                                                                                                                                                                                                                <  1:368973/195‑1 (MQ=255)
                                                                                                                                                   tGCCCCATTTCAGAGAAACGCCACCTAAAACACGGGCGGATATACCAACCAGCCCAAAACCAATCGGCGTCATTGTCTGCCAACTGGTCATACGTGAAGCCAGAATCATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGAAAATTATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGa                                                                                                                             <  2:1392356/288‑1 (MQ=255)
                                                                                                                                                   ggCCACCTGTCAGAGGAGCGCCACCTACAACGCAGGCGGAGCTAACAAGCCGATCGTAACCATTCGTCGTCATTGGCTGCGCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGGCTGAGAG‑AACAAAGATAATAATTGTGGTGCGATCAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCAAATCGCCAGGGTGTTACGACCAAAGGTGGTTGTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGa                                                                                                                             <  2:1078213/287‑1 (MQ=255)
                                                                                                                                                                  aaaCGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTgttgcccggaagat                                                                                                             >  1:1028221/1‑276 (MQ=255)
                                                                                                                                                                          ccTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGt                                                                                                     <  1:1339706/289‑1 (MQ=255)
                                                                                                                                                                               aaggagggCGGAAATAACAATCAGCACATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCg                                                                                                <  2:1806787/283‑1 (MQ=255)
                                                                                                                                                                               aaCGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCg                                                                                                <  1:928113/289‑1 (MQ=255)
                                                                                                                                                                               aaCGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCg                                                                                                <  1:1807491/289‑1 (MQ=255)
                                                                                                                                                                                    aagggtagaTAACATTCAGCGCATAACCAATAGACGTCATGGGATGCCCACTGGTCAGACGTGAAGCCAGAAGAATTCCGGCTATCGCTGATAACAGGCC‑TGGAGAAACAAAGATAATAATTTTGGTGCTAACAACCGGTACACCCGCCAGACGCGCGGCCTCGTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAAcc                                                                                           <  2:1045020/283‑1 (MQ=255)
                                                                                                                                                                                    aGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAgg                                                                                                                                                                                   <  2:1358794/201‑1 (MQ=255)
                                                                                                                                                                                     ggCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCa                                                                                          >  1:298601/1‑289 (MQ=255)
                                                                                                                                                                                       gggAGAGAACAATCAGATCTGAACCAGTGGAAGTAATTGGCGGCCCACTGGGCATACGTGAAGCCAGAATAAATCCGGCTAGCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGGTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAAcc                                                                                           <  2:1907792/285‑1 (MQ=255)
                                                                                                                                                                                       cGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAAcc                                                                                           <  2:768040/286‑1 (MQ=255)
                                                                                                                                                                                                      gCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTc                                                                         >  1:934459/1‑289 (MQ=255)
                                                                                                                                                                                                        tCATAACCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTc                                                                         <  1:515684/287‑1 (MQ=255)
                                                                                                                                                                                                            aaCCAATCGACGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCATCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTc                                                                         >  2:29790/1‑283 (MQ=255)
                                                                                                                                                                                                                 atcgacGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCgcagg                                                                                                                   >  2:1447222/6‑236 (MQ=255)
                                                                                                                                                                                                                 atcgacGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATa                                                               >  1:1421537/6‑288 (MQ=255)
                                                                                                                                                                                                                 atcgacGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATa                                                               >  1:70254/6‑288 (MQ=255)
                                                                                                                                                                                                                 atccacGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGAGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATGGGCGCCGGCAGACCGAACCAGTTGGCGGAACCAAGGGGAAAGAAGCTGTCAACTTCGaaa                                                               >  1:847438/6‑286 (MQ=255)
                                                                                                                                                                                                                      cGTCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCa                                                                                                                                                                                       >  1:617995/1‑163 (MQ=255)
                                                                                                                                                                                                                        tCATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATaccgacc                                                         >  1:1401834/1‑287 (MQ=255)
                                                                                                                                                                                                                         cATTGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCt                                                      <  1:855437/289‑1 (MQ=255)
                                                                                                                                                                                                                            tGGCTGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTa                                                   <  1:320255/289‑1 (MQ=255)
                                                                                                                                                                                                                               ccgCCCACTGGGCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGCTACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTAcc                                                 <  2:1924274/286‑1 (MQ=255)
                                                                                                                                                                                                                                tgaccACTCGACATACGTTAAGCCAGAATAAGTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCTCCCGCTTTACCGt                                               <  2:678300/286‑1 (MQ=255)
                                                                                                                                                                                                                                tGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGt                                               <  2:70254/289‑1 (MQ=255)
                                                                                                                                                                                                                                tGCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGt                                               <  1:1711505/289‑1 (MQ=255)
                                                                                                                                                                                                                                 gCCCACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGAAGCAAACCAAAGATAATCAGAAACGCAACGGTGAGCCAGATCGGCCACAGCCGACCGAACCAGTTAGGTTAACGAACGGCAAAGACAGATGCATCTTCGAGACCACCCGATGTACCg                                                >  2:50510/1‑287 (MQ=255)
                                                                                                                                                                                                                                  cccACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCg                                                >  1:1512165/1‑286 (MQ=255)
                                                                                                                                                                                                                                   ccACTGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCggctg                                            >  1:1800316/1‑285 (MQ=255)
                                                                                                                                                                                                                                     aCTGGTCATACGTGAAGCCAGAATAATTCAGGCTATAGCTGATACCAGGCCTGAGGG‑AACAAAGATAATAATGTTGGTGCAAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTGTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGCCGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAaagaag                                                                              <  2:1210195/253‑1 (MQ=255)
                                                                                                                                                                                                                                       tGGTCATACGTGAAGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTctgaaat                                        >  1:1868711/1‑283 (MQ=255)
                                                                                                                                                                                                                                           catacgtgaaGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAatgatg                                    <  1:671695/280‑1 (MQ=255)
                                                                                                                                                                                                                                           catacgtgaaGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAatgat                                     <  1:436634/279‑1 (MQ=255)
                                                                                                                                                                                                                                              acgtgaaGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTAc                                 >  1:1929399/7‑289 (MQ=255)
                                                                                                                                                                                                                                                 tgaaGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGcc                              >  2:823703/4‑289 (MQ=255)
                                                                                                                                                                                                                                                   aaGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTa                                                   >  2:1898762/2‑266 (MQ=255)
                                                                                                                                                                                                                                                   aaGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAg                            >  1:1374519/2‑289 (MQ=255)
                                                                                                                                                                                                                                                   aaGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAg                            >  1:456348/2‑289 (MQ=255)
                                                                                                                                                                                                                                                   aaGCCAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAg                            >  2:1108427/2‑289 (MQ=255)
                                                                                                                                                                                                                                                        aGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAGACCTc                       >  1:1038305/1‑289 (MQ=255)
                                                                                                                                                                                                                                                        aGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGAGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTATCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAGACCTc                       >  1:1281213/1‑289 (MQ=255)
                                                                                                                                                                                                                                                              atTCCGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCCTTCATCCTCGATACCGACCGCGTTACCGGCTGAAATGGAGTACGCCAGACCGCGAAcca                 >  2:285709/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                 ccGGCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAGACCTCGAACAATCt              <  1:1006865/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                    gCTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAGACCTCGAACAATCTGc            >  2:1843138/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                     cTATCGCTGATACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAGACCTCGAACAATCTGCa           <  1:1899533/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                             gatACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAGACCTCGAACAATCTGCATCGTTGCCa  <  1:1211873/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                             gatACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAGACCTCGAACAATCTGCATCGTTGCCa  <  1:1184918/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                             gatACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAGACCTCGAACAATCTGCATCGTTGCCa  <  1:334592/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                             gatACCAGGCCTGAGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAGACCTCGAACAATCTGCATCGTTGCCa  <  2:915813/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                              atACCAGGCCTGAGAG‑AACAAAGAGAATAATTGTGGTGCGACCAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAGACCTCGAACAATCTGCATCGTTGCCa  <  2:786718/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                    
GCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATAACTGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATGCTCGTCATTGGCTGCCCACTGGTCATACGACTAGCCAGAATAATTCCGGCTATCGCACTTACCAGGCCACTGAG‑AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCACTAATGATGTACGCCAGACCTCGAACAATCTGCATCGTTGCCA  >  CP042583/1646611‑1647168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A