Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,495,899 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,495,8990AT98.6% 680.5 / NA 289intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (151/132);  minor base C (4/0);  total (156/133)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,495,9000GC99.3% 684.4 / NA 289intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (154/133);  minor base A (2/0);  total (156/133)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,495,9010TA99.7% 692.3 / NA 289intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (155/133);  minor base C (1/0);  total (156/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

GCTTCACTAAACGCATATTAAAAATCAGAAAAACTGTAGTTTAGCCGATTTAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGAGCCAACATAACGAAAAGAACCCACATCAGCACCAGAGTCCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACAGTCTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACAGTCTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCCACTTTGACCATGAGCG  >  CP042583/1495620‑1496179
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                      
   tCACTAAACGCATATTAAAAATCAGAAAAACTGTAGTTTAGCCGATTTAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTg                                                                                                                                                                                                                                                                              >  1:17651/1‑289 (MQ=255)
          aCGCATATTAAAAATCAGAAAAACTGTAGTTTAGCCGATTTAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACg                                                                                                                                                                                                                                                                        >  1:22961/1‑288 (MQ=255)
              atatTAAAAATCAGAAAAACTGTAGTTTAGCCGATTTAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGctct                                                                                                                                                                                                                                                                   <  2:17651/289‑1 (MQ=255)
               tatTAAAAATCAGAAAAACTGTAGTTTAGCCGATTTAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGCTGGACTCCCTGGCACCTGAGGCCGGCcctc                                                                                                                                                                                                                                                                  >  2:259725/1‑289 (MQ=255)
                             aaaaCTGTAGTTTAGCCGATTTAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTTACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGc                                                                                                                                                                                                                                                     <  2:151220/288‑1 (MQ=255)
                             aaaaCTGTAGTTTAGCCGATTTAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGc                                                                                                                                                                                                                                                     <  1:2043701/288‑1 (MQ=255)
                                 cTGTAGTTTAGCCGATTTAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAAc                                                                                                                                                                                                                                                <  2:2614584/289‑1 (MQ=255)
                                     aGTTTAGCCGATTTAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCaa                                                                                                                                                                                                                                             <  1:323047/288‑1 (MQ=255)
                                            ccGATTTAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCa                                                                                                                                                                                                                                     >  1:163319/1‑289 (MQ=255)
                                             cGATTTAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAg                                                                                                                                                                                                                                    <  1:937572/289‑1 (MQ=255)
                                              gATTTAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTATCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGCGGACGGCTCTCATCGTCCAGCGGCGGAACCAACCCCGCCAgg                                                                                                                                                                                                                                   >  2:420469/1‑289 (MQ=255)
                                                tttAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTg                                                                                                                                                                                                                                 <  1:1800068/289‑1 (MQ=255)
                                                  tAGCCCCCGTACGTCCCCGTTTGGGGGTATTTCCTAAAACCGTTTCCAGGATAGTTCCCCAAGCGGGGTCTGGCGTGGT‑GTCTATTGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGca                                                                                                                                                                                                                               <  2:44860/289‑1 (MQ=255)
                                                        ttGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCAt                                                                                                                                                                                                                                                                <  1:2227927/249‑1 (MQ=255)
                                                         tGTACGTCCCGGTTTGCGTGTATTTCATAAACCCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTa                                                                                                                                                                                                                        <  2:743009/289‑1 (MQ=255)
                                                               tCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCGGAACCAAAACCGCCAGGTGCACAACCTACCGcc                                                                                                                                                                                                                   >  1:595966/1‑288 (MQ=255)
                                                                cccGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGc                                                                                                                                                                                                                                                                      >  1:2531587/1‑236 (MQ=255)
                                                                cccGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGAACCTGAGGACGGCTCTCATCGTCCATCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGccc                                                                                                                                                                                                                  >  1:1542222/1‑288 (MQ=255)
                                                                  cGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCCCCCCAg                                                                                                                                                                                                               >  1:1436934/1‑289 (MQ=255)
                                                                    cTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAggg                                                                                                                                                                                                             >  1:1146820/1‑289 (MQ=255)
                                                                    cTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAggg                                                                                                                                                                                                             >  1:851202/1‑289 (MQ=255)
                                                                     tttGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGTAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTa                                                                                                                                                                                                                        >  1:677739/1‑277 (MQ=255)
                                                                         cGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAg                                                                                                                                                                                                           >  1:1269331/1‑286 (MQ=255)
                                                                         cGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGAACAAAACAGCGGCCCTTTCAGTAATAAAATAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACACCAGCACCAGTCACTACTACACGATGCCAGCGAACCGAAACCGGGGATGGACTCACTGGCACCTTAAGACGGCTCCCCTGGTCCAGCGGTCGAACCAACACCGCAAGCTGCATCACCTTCCACCCCacaaatcc                                                                                                                                                                                                         >  2:896119/1‑281 (MQ=255)
                                                                                     aaCACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGcc                                                                                                                                                                                                                                      >  2:2155170/1‑247 (MQ=255)
                                                                                          cGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATAcgc                                                                                                                                                                                       >  1:1353403/1‑289 (MQ=255)
                                                                                            tttCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGc                                                                                                                                                                                                  >  1:475690/1‑276 (MQ=255)
                                                                                            tttCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGt                                                                                                                                                                                     >  1:1412732/1‑289 (MQ=255)
                                                                                            tttCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCCCTCATCGTCCAGCGGCTGAACCAACACCGCCACGTGCACAACCTACCGCCCCAGGGAGCCTTAAAGCCCCTTATAcgcct                                                                                                                                                                                     >  2:1306270/1‑287 (MQ=255)
                                                                                              tCCAGAATGGTCTCCGAAGCGGAATCTGGC‑TGGTGGTCTATAGTTAGAGAA‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGAGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGcaca                                                                                                                                                                                                                             <  1:1468764/247‑1 (MQ=255)
                                                                                                    aTAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGcc                                                                                                                                                                                                                                      >  1:1516853/1‑232 (MQ=255)
                                                                                                        tctcCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAgg                                                                                                                                                                                                                                                                           >  2:2713904/1‑191 (MQ=255)
                                                                                                        tctcCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAgg                                                                                                                                                                                                                                                                           <  1:2713904/191‑1 (MQ=255)
                                                                                                        tctcCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCa                                                                                                                                                                                                                <  2:1978547/250‑1 (MQ=255)
                                                                                                        tctcCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACtt                                                                                                                                                                         >  2:2545085/1‑289 (MQ=255)
                                                                                                              aaGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGcc                                                                                                                                                                                                                                      >  1:2217411/1‑222 (MQ=255)
                                                                                                              aaGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGcc                                                                                                                                                                                                                                      <  2:2217411/222‑1 (MQ=255)
                                                                                                                        ttgc‑tggTTGTCTATAGTTAGAGAG‑TTTTTTAACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACg                                                                                                                                                         <  2:493599/287‑1 (MQ=255)
                                                                                                                               gtggtCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGcac                                                                                                                                                                                                                              <  2:2266035/214‑1 (MQ=255)
                                                                                                                               gtggtCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCa                                                                                                                                                   >  2:300715/1‑289 (MQ=255)
                                                                                                                                    cTATAGTGAGAGAA‑TGATTTGACCAAACAACCAGCACTTGCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGGTTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGc                                                                                                                                              <  2:1539418/289‑1 (MQ=255)
                                                                                                                                         tataaagag‑GTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAAGGTCGCAAAATAACGAAAAGAACCCACAGCGGCACCAGTCCCCACTAAACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTAGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGa                                                                                                                                         <  2:1602710/284‑1 (MQ=255)
                                                                                                                                            agagagtTTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAAACTAATTCCCTGGAAGACGTACGCAAAGGCAGTGaaa                                                                                                                                       >  2:1640791/1‑289 (MQ=255)
                                                                                                                                            agagag‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTa                                                                                                                                                                                                                        <  1:2343014/207‑1 (MQ=255)
                                                                                                                                            agagag‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGCTACGCGTAACGAAAAACTTAATTCTATGGAAGACGTACGCAAAGGCAGGGaaaa                                                                                                                                      >  2:1346967/1‑289 (MQ=255)
                                                                                                                                            agagag‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGCCGTACGCAAAGGCAGTGaaaa                                                                                                                                      >  1:2453985/1‑289 (MQ=255)
                                                                                                                                            agagag‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGaaaa                                                                                                                                      >  2:571681/1‑289 (MQ=255)
                                                                                                                                            agagag‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCGCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTCAATTCTCTGGAAGAAGTACGCAAAGGCatacaaaa                                                                                                                                      >  2:1741318/1‑282 (MQ=255)
                                                                                                                                            agagag‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCCCTCATCGTCCAGCGGCTGACCCAACACCCCCAGGTGCACAACCTCCCGCCCAAGGGAGCCTGAAAGCCCCTGACACGCGTAACGAAAAACTTAATTCCCTGGAAGACGTACGCAAAGGCAGGAaaaa                                                                                                                                      >  2:669021/1‑289 (MQ=255)
                                                                                                                                             gagag‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCt                                                                                                                                                                                                                                                                               >  2:2600417/1‑151 (MQ=255)
                                                                                                                                              agag‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGcc                                                                                                                                                                                                                                      >  1:503185/1‑191 (MQ=255)
                                                                                                                                              agag‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGcc                                                                                                                                                                                                                                      <  2:503185/191‑1 (MQ=255)
                                                                                                                                                   ttttttGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTgg                                                                                                                                                               >  1:1068828/1‑258 (MQ=255)
                                                                                                                                                   ttttttGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGAACAACCTACCGCCCCCGGGAGCCTGAAAGCCCCTGCTACGCGTAACGAAAAACTTAATTCCCTGGAAGACGTACGCAAAGGCAGTGAAAATTATgc                                                                                                                                >  2:231755/1‑289 (MQ=255)
                                                                                                                                                     ttttGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATgc                                                                                                                                >  1:2391602/1‑287 (MQ=255)
                                                                                                                                                      tttGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCt                                                                                                                             <  2:1362946/289‑1 (MQ=255)
                                                                                                                                                        tGACCAAAACAGCGGCCACTACAATAAAAAATTAAGGAGACGCGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTAACCGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGa                                                                                                                           <  2:2528275/289‑1 (MQ=255)
                                                                                                                                                          aCCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGAcc                                                                                                                         <  2:1931058/289‑1 (MQ=255)
                                                                                                                                                           ccAAAACAGCGGCCCGTTCAGTAAGAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCGCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGAcc                                                                                                                         <  2:1892368/288‑1 (MQ=255)
                                                                                                                                                                    cGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCATGGGGCCTGAAAGCCCCTGATACGCGCAACGAAAAACTTAATTCTCTGGAAGACGGACGCAAAGCAAGTGAAAATTATGCGCTGACCCCTaaaccgg                                                                                                               >  2:2459014/1‑284 (MQ=255)
                                                                                                                                                                    cGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCCgg                                                                                                               >  2:2341916/1‑289 (MQ=255)
                                                                                                                                                                    cGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAgg                                                                                                               >  2:938304/1‑289 (MQ=255)
                                                                                                                                                                                taataaATTAAGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATc                                                                                                    >  1:2153146/1‑288 (MQ=255)
                                                                                                                                                                                          aGGAGACGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGcc                                                                                                 <  1:354302/281‑1 (MQ=255)
                                                                                                                                                                                               aCGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGaa                                                                                                                                                                                                     <  2:77815/176‑1 (MQ=255)
                                                                                                                                                                                               aCGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGaa                                                                                                                                                                                                     >  1:77815/1‑176 (MQ=255)
                                                                                                                                                                                               aCGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAACGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAAc                                                                                     >  1:1596962/1‑288 (MQ=255)
                                                                                                                                                                                               aCGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCt                                                                                                                             >  2:1662204/1‑248 (MQ=255)
                                                                                                                                                                                               aCGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCt                                                                                                                             <  1:1662204/248‑1 (MQ=255)
                                                                                                                                                                                               aCGAGTTCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAAc                                                                                     >  1:1837673/1‑288 (MQ=255)
                                                                                                                                                                                                    ttCAATGTCGCAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAAct                                                                                    >  2:1245923/1‑283 (MQ=255)
                                                                                                                                                                                                     tcaatgtcgcAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAActcact                                                                                <  1:2545085/278‑6 (MQ=255)
                                                                                                                                                                                                            cgcAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCg                                                                        <  2:1837673/286‑1 (MQ=255)
                                                                                                                                                                                                              cAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCt                                                                                                                             <  1:1018828/233‑1 (MQ=255)
                                                                                                                                                                                                              cAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCt                                                                                                                             >  2:1018828/1‑233 (MQ=255)
                                                                                                                                                                                                              cAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCcg                                                                                                >  1:1293055/1‑262 (MQ=255)
                                                                                                                                                                                                              cAACATAACGAAAAGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCcg                                                                                                <  2:1293055/262‑1 (MQ=255)
                                                                                                                                                                                                                           aGAACCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCaa                                                         >  2:641869/1‑288 (MQ=255)
                                                                                                                                                                                                                            aaaCCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACg                                                       <  1:2620514/288‑1 (MQ=255)
                                                                                                                                                                                                                              aCCCACATCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACg                                                       <  1:1315125/287‑1 (MQ=255)
                                                                                                                                                                                                                                  acaTCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGctgctg                                                 <  2:552529/289‑1 (MQ=255)
                                                                                                                                                                                                                                    aTCAGCACCAGTCACCACTACCCGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGTACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGa                                               <  1:2598044/289‑1 (MQ=255)
                                                                                                                                                                                                                                    aTCAGCACCAGTCACCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGa                                               <  2:350506/289‑1 (MQ=255)
                                                                                                                                                                                                                                              gtcaccACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAAtt                                                                                                                                                                     <  1:2336924/157‑1 (MQ=255)
                                                                                                                                                                                                                                              gtcaccACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATc                                                                                                                  >  1:1943482/5‑212 (MQ=255)
                                                                                                                                                                                                                                              gtcaccACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCCGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCGTCTAGAAGATTTTAtt                                     >  1:63487/5‑289 (MQ=255)
                                                                                                                                                                                                                                              gtcaccACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTTCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTAt                                      >  2:1165368/5‑288 (MQ=255)
                                                                                                                                                                                                                                              gtcaccACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTAt                                      >  1:1092270/5‑288 (MQ=255)
                                                                                                                                                                                                                                              gtcaccACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTAt                                      >  1:1233895/5‑288 (MQ=255)
                                                                                                                                                                                                                                               tcaccACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTc                                    <  2:851202/286‑1 (MQ=255)
                                                                                                                                                                                                                                                   cACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCa                                                                                         <  2:603157/232‑1 (MQ=255)
                                                                                                                                                                                                                                                      tACACGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTTCCGGTAGCCGTGGTCCAACGCTGCTGGAAGCTTGTATTCGGCGCga                             >  2:1057941/1‑289 (MQ=255)
                                                                                                                                                                                                                                                        cacGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACGCCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCgaga                           >  1:623861/1‑289 (MQ=255)
                                                                                                                                                                                                                                                        cacGATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAAt                                                                                                                                                                      >  2:2086733/1‑150 (MQ=255)
                                                                                                                                                                                                                                                           gATTCCAGCGAAGCGAAACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGAACAAAACCACCTGCGTGCCGGTAGCCCTGGTCCAACGCTGCTGGAAGCTTTTATTCTGCGGGAGaaaa                        >  2:1410570/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                  gcgaagcgaaACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATgcg                                                                                                                               >  1:28284/1‑179 (MQ=255)
                                                                                                                                                                                                                                                                   cgaagcgaaACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCa                                                                                                                        <  2:1450417/185‑1 (MQ=255)
                                                                                                                                                                                                                                                                     aagcgagAACGGGGATGGACGCACTGGCACCTGGGGACTGCTCACATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCACCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCCACt               <  1:7565/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                       gcgaaACCGGGGATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCCACTTTg            >  1:1992213/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                  gATGGACTCACTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACTCACTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCCACTTTGACCATGAgcg  <  2:1087215/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                      
GCTTCACTAAACGCATATTAAAAATCAGAAAAACTGTAGTTTAGCCGATTTAGCCCCTGTACGTCCCGCTTTGCGTGTATTTCATAACACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGC‑TGGTGGTCTATAGTTAGAGAG‑TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCAATGAGCCAACATAACGAAAAGAACCCACATCAGCACCAGAGTCCACTACACGATTCCAGCGAAGCGAAACCGGGGATGGACAGTCTGGCACCTGAGGACGGCTCTCATCGTCCAGCGGCTGAACCAACACCGCCAGGTGCACAACCTACCGCCCCAGGGAGCCTGAAAGCCCCTGATACGCGTAACGAAAAACTTAATTCTCTGGAAGACGTACGCAAAGGCAGTGAAAATTATGCGCTGACCACTAATCAGGGCGTGCGCATCGCCGACGATCAAAACAGTCTGCGTGCCGGTAGCCGTGGTCCAACGCTGCTGGAAGATTTTATTCTGCGCGAGAAAATCACCCACTTTGACCATGAGCG  >  CP042583/1495620‑1496179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A