Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,532,384 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,532,3840AT99.7% 857.4 / NA 360intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (180/178);  minor base G (0/1);  total (180/180)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,532,3850GC99.2% 842.8 / NA 360intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/2);  major base C (178/177);  minor base A (2/1);  total (180/180)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,532,3860CG99.7% 861.8 / NA 360intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (180/179);  total (180/180)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 360 total aligned reads displayed.

TGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATAGCATTGGTGTTCATACTGATGGTGTCAGTACTAGTCCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATAGCACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGTTACCTGCACCGCTGGCCAGCGTAGCCTCTACCGT  >  CP042583/1532109‑1532664
                                                                                                                                                                                                                                                                                    |||                                                                                                                                                                                                                                                                                       
tggcggcatCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCa                                                                                                                                                                                                                                                                              >  1:1271427/1‑289 (MQ=255)
  gcggcatCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAg                                                                                                                                                                                                                                                                             <  2:947422/288‑1 (MQ=255)
                 tACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGccc                                                                                                                                                                                                                                                             <  1:560819/289‑1 (MQ=255)
                    tGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGACTGATAAAATTGCCCAgg                                                                                                                                                                                                                                                          >  1:269531/1‑289 (MQ=255)
                       aTTTCCACGCCAGCTAATTCCATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGc                                                                                                                                                                                                                                                        <  2:41428/288‑1 (MQ=255)
                       aTTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGc                                                                                                                                                                                                                                                        <  1:373934/288‑1 (MQ=255)
                       aTTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGc                                                                                                                                                                                                                                                        <  1:395737/288‑1 (MQ=255)
                       aTTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGc                                                                                                                                                                                                                                                        <  1:1459015/288‑1 (MQ=255)
                       aTTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGc                                                                                                                                                                                                                                                        <  2:228497/288‑1 (MQ=255)
                       aTTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGc                                                                                                                                                                                                                                                        <  2:2252121/288‑1 (MQ=255)
                                ccAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGc                                                                                                                                                                                                                                                        <  1:2270853/279‑1 (MQ=255)
                                    cTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCg‑g                                                                                                                                                                                                                                         >  1:1549799/1‑289 (MQ=255)
                                     tgattcatttGCGGCAAAACACAGCACCCGGACGAGTTGTATCGGTATTTTCGGCGTGATCGCCGCCGGATAAAATAGTCTGG‑ATTCGATTGGTGTTCATACGGCTGGGGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑Gt                                                                                                                                                                                                                                        <  2:1488931/281‑1 (MQ=255)
                                     tcaTTACCTTTTGGCTAACCCCAGCACCATGACCGGTTCTATCGGGATCTGCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCg                                                                                                                                                                                                                                           <  2:2663555/285‑1 (MQ=255)
                                     tAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑Gt                                                                                                                                                                                                                                        <  1:619521/289‑1 (MQ=255)
                                         taccgtgtgGCTAGCCCCATCACCCTTGTCGTTTCTCTCGGTATCTTCGGCGTAATCGCCACCGTAGAAACTAGTCTGGAATAG‑ATTGGTTTTCCTACTGCTGGTGTCGCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCTCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACTCTGGTTGCTGATGCGGGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCGACGTCTGGACCG‑GTCAgg                                                                                                                                                                                                                                    <  2:1708637/284‑1 (MQ=255)
                                              tgtgGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑TTCAggtcgca                                                                                                                                                                                                                               >  2:1502993/1‑284 (MQ=255)
                                                tgGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGc                                                                                                                                                                                                                                                        >  2:816390/1‑263 (MQ=255)
                                                      aCCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTACCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTa                                                                                                                                                                                                                        >  1:810856/1‑288 (MQ=255)
                                                            gCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGc                                                                                                                                                                                                                                                        <  1:1890073/251‑1 (MQ=255)
                                                            gCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGc                                                                                                                                                                                                                                                        >  2:1890073/1‑251 (MQ=255)
                                                                  tGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTTTAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGAGCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGa                                                                                                                                                                                                           >  2:345186/1‑289 (MQ=255)
                                                                  tGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGCTTGATAAAATTGCCCAGGGCCCCGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGa                                                                                                                                                                                                           >  2:2058953/1‑289 (MQ=255)
                                                                  tGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGa                                                                                                                                                                                                           >  1:2290264/1‑289 (MQ=255)
                                                                  tGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACAG‑GTCCGGATGCAAAAGCAAACGGGCTGGTAGa                                                                                                                                                                                                           >  2:1599437/1‑289 (MQ=255)
                                                                  tGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGCCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCCTTATCACGCTGGTTGCTGCTGCGCGTCATTCGACGCCGGAGCAGATTGACAAAATTGCCCAGGGCCACGTCTGGACCG‑CTCAGGCTCCGAAACCTACCGGCCCGGTcac                                                                                                                                                                                                           >  2:2136305/1‑287 (MQ=255)
                                                                    aCCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGc                                                                                                                                                                                                                                                        <  1:717716/243‑1 (MQ=255)
                                                                        gTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCa                                                                                                                                                                                                                               <  2:907685/263‑1 (MQ=255)
                                                                                   aTCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAg                                                                                                                                                                                                                                     <  2:1732000/246‑1 (MQ=255)
                                                                                   aTCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAg                                                                                                                                                                                                                                     >  1:1732000/1‑246 (MQ=255)
                                                                                   aTCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCg                                                                                                                                                                                          >  1:2013202/1‑289 (MQ=255)
                                                                                   aTCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCg                                                                                                                                                                                          >  1:796695/1‑289 (MQ=255)
                                                                                   aTCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCGCGGGGAGTTCg                                                                                                                                                                                          >  1:2215775/1‑289 (MQ=255)
                                                                                            gTGATCACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGa                                                                                                                                                                                                                                              >  2:719614/1‑229 (MQ=255)
                                                                                            gTGATAACCACCGTAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCg                                                                                                                                                                                  <  1:1909223/288‑1 (MQ=255)
                                                                                                         tAGAAAATAGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCCCGGGGATTTCGATGGAGCGGTCGCCAAagcag                                                                                                                                                                     >  2:2329455/1‑288 (MQ=255)
                                                                                                                 aGTCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTg                                                                                                                                                             >  2:2464013/1‑288 (MQ=255)
                                                                                                                   tCTGG‑ATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCa                                                                                                                                                          >  1:1699486/1‑289 (MQ=255)
                                                                                                                         attcgaTTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGa                                                                                                                                                                                                           <  2:842497/230‑1 (MQ=255)
                                                                                                                         attcgaTTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGa                                                                                                                                                                                                           >  1:842497/6‑235 (MQ=255)
                                                                                                                              aTTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGTTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGGCGATAGTCTCGGGGATTTCCACTATGCGGTCCACAAAGAAGTCGAGCTGGCAAAAGTGaatg                                                                                                                                                >  2:1333717/1‑287 (MQ=255)
                                                                                                                              aTTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAg                                                                                                                                                                                                                                                                             >  1:2186143/1‑165 (MQ=255)
                                                                                                                              aTTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCg                                                                                                                                                                                                   <  2:2701804/238‑1 (MQ=255)
                                                                                                                              aTTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAAc                                                                                                                                                >  2:662916/1‑289 (MQ=255)
                                                                                                                              aTTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATGGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCACAAGCGAAAc                                                                                                                                                >  1:934391/1‑289 (MQ=255)
                                                                                                                                 ggTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGt                                                                                                                                             <  1:1865794/289‑1 (MQ=255)
                                                                                                                                  gtgtTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAagcag                                                                                                                                                                     >  2:2380360/1‑264 (MQ=255)
                                                                                                                                  gtgtTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAagcag                                                                                                                                                                     <  1:2380360/264‑1 (MQ=255)
                                                                                                                                  gtgtTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATGTCGATGAGGCGGTTGCAAAAGCAGCAGAGCTGGCAAAAGTGAAACAGta                                                                                                                                            >  2:502467/1‑288 (MQ=255)
                                                                                                                                   tgtTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTg                                                                                                                                            >  2:2514011/1‑288 (MQ=255)
                                                                                                                                    gtTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGc                                                                                                                                          <  1:274540/289‑1 (MQ=255)
                                                                                                                                       cATACTGATGGTGTCTAAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATa                                                                                                                                                                                                         >  1:658149/1‑223 (MQ=255)
                                                                                                                                                  tgtctcaacttcaccGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTa                                                                                                                             <  1:2417880/275‑1 (MQ=255)
                                                                                                                                                    tctcaacttcaccGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGt                                                                                                                          >  1:143053/12‑289 (MQ=255)
                                                                                                                                                    tctcaacttcaccGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGt                                                                                                                          >  1:497461/12‑289 (MQ=255)
                                                                                                                                                    tctcaacttcaccGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGt                                                                                                                          >  2:1938058/12‑289 (MQ=255)
                                                                                                                                                      tcaacttcaccGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTtg                                                                                                                        >  1:295803/10‑289 (MQ=255)
                                                                                                                                                            tcaccGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGaa                                                                                                                   >  1:447939/4‑288 (MQ=255)
                                                                                                                                                              accGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGc                                                                                                                                                                                                                                                        >  1:2566526/2‑154 (MQ=255)
                                                                                                                                                                  cTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAACTGGCAAAAGGGAAACAGTTGCATCTGGAATATTACTTTGATGAACAGACct                                                                                                            >  2:1418869/1‑289 (MQ=255)
                                                                                                                                                                           gTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACa                                                                                                   <  2:143053/289‑1 (MQ=255)
                                                                                                                                                                           gTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACa                                                                                                   <  1:2478119/289‑1 (MQ=255)
                                                                                                                                                                             ttCTATAACCAGGGAACGGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACaaa                                                                                                 <  2:1021029/289‑1 (MQ=255)
                                                                                                                                                                              tCTAACACCAGGGCACTGCCGCCGGAAGGTAAGCTGATGATGCAGTTAAGAATTGGGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAg                                                                                                <  2:1244010/289‑1 (MQ=255)
                                                                                                                                                                                     ccAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGc                                                                                                                                                                                                                                                        <  2:1173793/131‑1 (MQ=255)
                                                                                                                                                                                         ggCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGa                                                                                                                                                                                                           >  2:2030350/1‑171 (MQ=255)
                                                                                                                                                                                         ggCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTTTTCGACAAAGGTATGGaaaac                                                                                     >  2:1830242/1‑289 (MQ=255)
                                                                                                                                                                                         ggCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACa                                                                                                   >  1:204184/1‑275 (MQ=255)
                                                                                                                                                                                          gcccTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGacaac                                                                                     <  1:2094605/285‑1 (MQ=255)
                                                                                                                                                                                          gCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGTAGCAGATTTATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGacaac                                                                                     <  1:1638857/288‑1 (MQ=255)
                                                                                                                                                                                             cTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGt                                                                                 <  1:1953966/289‑1 (MQ=255)
                                                                                                                                                                                              tGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACACTCTTCGACAAAGTGATGGAAAACATg                                                                                  >  2:1999039/1‑287 (MQ=255)
                                                                                                                                                                                                ccgccgGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACCTTGATGAACCGACCTTCTTCGACAAAGGGATGGACAACATTTCTg                                                                              >  2:772980/1‑289 (MQ=255)
                                                                                                                                                                                                    cgGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGtt                                                                           <  1:2633017/288‑1 (MQ=255)
                                                                                                                                                                                                    cgGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGtt                                                                           <  1:2175979/288‑1 (MQ=255)
                                                                                                                                                                                                                tgatgaTGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCa                                                                <  2:2401198/287‑1 (MQ=255)
                                                                                                                                                                                                                         aGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAGTATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGa                                                     >  2:1761121/1‑289 (MQ=255)
                                                                                                                                                                                                                           ttAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGAt                                                    >  2:969821/1‑288 (MQ=255)
                                                                                                                                                                                                                            tAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATg                                                   <  1:1935474/288‑1 (MQ=255)
                                                                                                                                                                                                                                cATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCACAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGCATATTACGTTGATGATCCGACCTTCTTCGACAAAGCGATGGACAACATGTCTGGTTCTGTCCGGGCAATTTTGCCAGATGCGtt                                               >  2:2638178/1‑288 (MQ=255)
                                                                                                                                                                                                                                 aTTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTcc                                             <  1:969821/289‑1 (MQ=255)
                                                                                                                                                                                                                                   tgcaaagggctcaaaaCGTTTCTTCACGATGGTTGCGGACGGGCGTCATGCGAAGCCGGAGGAGATTGATACAGTTGCCCTGGGCGTGGACTGGAACGTGTC‑GGCTGCAAAAGCGAACGGGCTGGTAGGTAGTCCCGGAGATTTCGATGATGCGGTTGCCAAAGCAGTTTAGCTAGCAAAAGTGTAACCGTGGCATCTGGAAAACTACTTTGATGAACCGACCTGGTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCCGGCTATGTTGCCAGATGCGTTCCAg                                           <  2:1932796/276‑1 (MQ=255)
                                                                                                                                                                                                                                   tGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTa                                                                                                                             <  2:360879/207‑1 (MQ=255)
                                                                                                                                                                                                                                   tGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGTACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGGCTTCCAg                                           >  2:2576671/1‑289 (MQ=255)
                                                                                                                                                                                                                                          ggCTATAAGCGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGt                                    <  1:905043/289‑1 (MQ=255)
                                                                                                                                                                                                                                          ggCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGaca                                                                                       >  1:1500232/1‑238 (MQ=255)
                                                                                                                                                                                                                                               taAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACa                                                                                                   <  2:260937/221‑1 (MQ=255)
                                                                                                                                                                                                                                                       ttATCACGCTGGTTGCTGATGCGGGTCATTCGACGGCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGTTACCTGCACCGCt                       <  1:1347900/289‑1 (MQ=255)
                                                                                                                                                                                                                                                         aTCACGCTGGTTGCTGATGGCCGTCATTCGACGCCGGAGCAGATGGATAAAATTGCCCAGGGCAACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGTTACCTGCACCGCTgg                     <  2:448359/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             cGCTGGTTGCTGATGCGCGTCATTCGACACCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTACAGGCCATGTTACCTGCACCGCTGGcctc                 >  1:1087309/1‑287 (MQ=255)
                                                                                                                                                                                                                                                              gCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACaaa                                                                                                 >  1:2590824/1‑208 (MQ=255)
                                                                                                                                                                                                                                                                tGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGTTACCTGCACCGCTGGcctcggt              >  1:2651817/1‑284 (MQ=255)
                                                                                                                                                                                                                                                                  gTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTc                                                                                >  1:1759422/1‑221 (MQ=255)
                                                                                                                                                                                                                                                                  gTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATg                                                   <  1:1782548/250‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCAt                                      >  2:613170/1‑263 (MQ=255)
                                                                                                                                                                                                                                                                  gTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGTTACCTGCACCGCTGGcctcggta             >  1:1018968/1‑282 (MQ=255)
                                                                                                                                                                                                                                                                    tGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGc                                                                                                                                          <  2:280435/161‑1 (MQ=255)
                                                                                                                                                                                                                                                                    tGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGTTACCTGCACCGCTGGcctcggtagc           >  2:2211136/1‑280 (MQ=255)
                                                                                                                                                                                                                                                                            cgcgTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGTAGCCTCTACCGt  >  1:119251/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                            cgcgTCATTCGACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAAAATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGTTACCTGCCCCGCTGGCCTGGGTAGCCGCTAACGt  >  2:88122/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                    |||                                                                                                                                                                                                                                                                                       
TGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGG‑ATAGCATTGGTGTTCATACTGATGGTGTCAGTACTAGTCCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATAGCACGCCGGAGCAGATTGATAAAATTGCCCAGGGCCACGTCTGGACCG‑GTCAGGATGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCGCCAAAGCAGCAGAGCTGGCAAAAGTGAAACAGTGGCATCTGGAATATTACGTTGATGAACCGACCTTCTTCGACAAAGTGATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGTTACCTGCACCGCTGGCCAGCGTAGCCTCTACCGT  >  CP042583/1532109‑1532664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A