Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
715 |
38.4 |
1606192 |
96.6% |
1551581 |
120.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,908,514 |
A→G |
intergenic (‑148/+35) |
pstB ← / ← pstA |
phosphate ABC transporter ATPase/phosphate ABC transporter permease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,908,514 | 0 | A | G | 100.0%
| 107.3
/ NA
| 32 | intergenic (‑148/+35) | pstB/pstA | phosphate ABC transporter ATPase/phosphate ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/16); total (16/16) |
ATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAA > NC_000913/3908382‑3908623
|
aTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTa > 2:325911/1‑139 (MQ=255)
tCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGc < 2:465584/139‑1 (MQ=255)
aGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCaa > 2:728990/1‑139 (MQ=255)
gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCa > 2:641007/1‑133 (MQ=255)
gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCa < 1:641007/133‑1 (MQ=255)
ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc < 1:162248/139‑1 (MQ=255)
ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc > 2:162248/1‑139 (MQ=255)
ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc < 1:728990/139‑1 (MQ=255)
ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc < 2:512899/139‑1 (MQ=255)
ggCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCgtgt > 2:271156/1‑139 (MQ=255)
ggCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCgtgt > 1:648369/1‑139 (MQ=255)
gATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCGGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAtt > 1:174752/1‑139 (MQ=255)
ggCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCa < 1:271156/139‑1 (MQ=255)
aCCGTGTACAGAGTCAAAAAGGCGCTCGGCCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAGCCGGGCCGGCATTA‑CCACCCTGGTTTTTCTTCCGAAAAAACAACCCCCGCAAGAATGttaacca > 2:534668/1‑134 (MQ=16)
tgtaccgaagTAAAAAGGGCGCTGGGCCGGCCCCTTCGCCCCTCTGGGG‑GGCGGTTAAGGGGAAGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAAAATGTTcagcagcag < 1:534668/131‑1 (MQ=255)
gtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCg < 2:353293/73‑1 (MQ=255)
gtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCg > 1:353293/1‑73 (MQ=255)
gtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCaa < 1:244680/88‑1 (MQ=255)
gtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCaa > 2:244680/1‑88 (MQ=255)
gtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGt > 1:156004/1‑139 (MQ=255)
gtACAGAGTCAAAAAAGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGt > 2:741745/1‑139 (MQ=255)
gagTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGca < 1:630220/139‑1 (MQ=255)
gagTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGca < 2:641854/139‑1 (MQ=255)
gagTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGca < 2:6630/139‑1 (MQ=255)
agTCCAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAACAGTACGcac > 1:151824/1‑139 (MQ=255)
agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcac > 2:164368/1‑139 (MQ=255)
agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcac > 1:647031/1‑139 (MQ=255)
ttCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcaca < 1:325911/138‑1 (MQ=255)
gTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcaca < 1:164368/139‑1 (MQ=255)
tcggtcggTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAACAGTACGCACAGGGTAATGATCaa < 2:151824/139‑1 (MQ=255)
tctGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGca < 2:414467/104‑1 (MQ=255)
tctGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGca > 1:414467/1‑104 (MQ=255)
|
ATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTT‑CGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAA > NC_000913/3908382‑3908623
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
