Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I2 R1 777 59.2 2955938 92.5% 2734242 106.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,277,726 C→T F130F (TTC→TTT)  yhaV → toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,277,7260CT100.0% 192.9 / NA 59F130F (TTC→TTT) yhaVtoxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/24);  total (35/24)

GGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACT  >  NC_000913/3277601‑3277860
                                                                                                                             |                                                                                                                                      
ggTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCACTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTaa                                                                                                                           >  1:408124/1‑139 (MQ=255)
 gTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTaaa                                                                                                                          >  2:146683/1‑139 (MQ=255)
                      tcttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGa                                                                                                     <  1:457907/139‑1 (MQ=255)
                             ttATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaa                                                                                              >  1:786376/1‑139 (MQ=255)
                               ataGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAc                                                                                            >  1:1307391/1‑139 (MQ=255)
                                      aaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAcc                                                                                     >  2:1224923/1‑139 (MQ=255)
                                           agaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCaaa                                                                                                                                  >  2:549549/1‑89 (MQ=255)
                                           agaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCaaa                                                                                                                                  <  1:549549/89‑1 (MQ=255)
                                            gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGcc                                                                                                       <  2:57865/115‑1 (MQ=255)
                                            gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGcc                                                                                                       >  1:57865/1‑115 (MQ=255)
                                            gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCg                                                                                   >  2:364207/1‑135 (MQ=255)
                                            gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCg                                                                                   <  1:364207/135‑1 (MQ=255)
                                             aaaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGaaa                                                                              >  1:402206/1‑139 (MQ=255)
                                                  catcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTa                                                                                                                            <  2:707123/88‑1 (MQ=255)
                                                  catcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTa                                                                                                                            >  1:707123/1‑88 (MQ=255)
                                                   atcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCa                                                                                                                                    >  2:1087361/1‑79 (MQ=255)
                                                   atcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCa                                                                                                                                    <  1:1087361/79‑1 (MQ=255)
                                                     catTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAgaagaa                                                                      >  1:263283/1‑139 (MQ=255)
                                                        tCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAcc                                                                   >  1:1310968/1‑139 (MQ=255)
                                                            ggatggatGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                     <  1:134897/133‑1 (MQ=255)
                                                            ggatggatGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                     >  2:134897/1‑133 (MQ=255)
                                                            ggatggatGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAtt                                                               >  2:553474/1‑139 (MQ=255)
                                                             gatggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTg                                                              <  2:408124/139‑1 (MQ=255)
                                                               tggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGAt                                                            <  1:553474/139‑1 (MQ=255)
                                                                 gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGTCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                                                          >  2:172386/1‑139 (MQ=255)
                                                                 gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                                                                                                <  2:950754/101‑1 (MQ=255)
                                                                 gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                                                                                                >  1:950754/1‑101 (MQ=255)
                                                                   tgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGgtg                                                        <  2:489296/139‑1 (MQ=255)
                                                                          tGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACaa                                                 >  1:46965/1‑139 (MQ=255)
                                                                           gAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAt                                                                <  1:1070755/123‑1 (MQ=255)
                                                                           gAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAt                                                                >  2:1070755/1‑123 (MQ=255)
                                                                           gAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAt                                                >  1:873418/1‑139 (MQ=255)
                                                                            aaaaCACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATg                                               >  1:1179673/1‑139 (MQ=255)
                                                                            aaaaCACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATg                                               <  2:263283/139‑1 (MQ=255)
                                                                                      gcgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAaga                                                                                                                      <  1:87587/58‑1 (MQ=255)
                                                                                      gcgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAaga                                                                                                                      >  2:87587/1‑58 (MQ=255)
                                                                                       cgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                        <  2:1107779/87‑1 (MQ=255)
                                                                                       cgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                        >  1:1107779/1‑87 (MQ=255)
                                                                                               cGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGaa                                      <  2:1254994/129‑1 (MQ=255)
                                                                                               cGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGaa                                      >  1:1254994/1‑129 (MQ=255)
                                                                                                 gTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAt                                                                >  2:286928/1‑101 (MQ=255)
                                                                                                 gTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAt                                                                <  1:286928/101‑1 (MQ=255)
                                                                                                 gTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                                                          >  1:717795/1‑107 (MQ=255)
                                                                                                 gTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                                                          <  2:717795/107‑1 (MQ=255)
                                                                                                    aaaaaaCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                        <  1:66500/74‑1 (MQ=255)
                                                                                                    aaaaaaCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                        >  2:66500/1‑74 (MQ=255)
                                                                                                            gATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGc               >  1:973791/1‑139 (MQ=255)
                                                                                                             aTGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCa              <  2:786376/139‑1 (MQ=255)
                                                                                                              tGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                     <  1:870206/83‑1 (MQ=255)
                                                                                                              tGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                     >  2:870206/1‑83 (MQ=255)
                                                                                                                         tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTg                                                                                                         <  2:783419/36‑1 (MQ=255)
                                                                                                                         tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTg                                                                                                         >  1:783419/1‑36 (MQ=255)
                                                                                                                         tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGa                                                             >  2:1200692/1‑80 (MQ=255)
                                                                                                                         tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGa                                                             <  1:1200692/80‑1 (MQ=255)
                                                                                                                         tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACt  >  2:517719/1‑139 (MQ=255)
                                                                                                                         tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACt  >  1:885489/1‑139 (MQ=255)
                                                                                                                         tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACt  >  1:1025144/1‑139 (MQ=255)
                                                                                                                         tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCACACCCGAGAAACAGAAGAAACTCACTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGACATCCTTTCTCACt  >  2:901383/1‑139 (MQ=255)
                                                                                                                          aTTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGAcc                      <  2:111634/118‑1 (MQ=255)
                                                                                                                             |                                                                                                                                      
GGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACT  >  NC_000913/3277601‑3277860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A