Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,277,726	C→T	F130F (TTC→TTT)	yhaV →	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,726	0	C	T	100.0%	115.6 / NA	36	F130F (TTC→TTT)	yhaV	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/18); total (18/18)

AAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCT  >  NC_000913/3277594‑3277856

aaaaTTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCaaa                                                                                                                              <  2:975866/139‑1 (MQ=255)

         tGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTaaaa                                                                                                                     <  1:799484/139‑1 (MQ=255)

                                        aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAccc                                                                                      >  1:1198920/1‑139 (MQ=255)

                                                 gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACAtcctcc                                                                                                       <  1:1213367/113‑1 (MQ=255)

                                                 gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACAtcctcc                                                                                                       >  2:1213367/1‑113 (MQ=255)

                                                    aaaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGaaa                                                                          >  1:456741/1‑139 (MQ=255)

                                                      aGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACa                                                                        >  2:942665/1‑139 (MQ=255)

                                                                   ggatggatGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCa                                                                                                                                <  2:720899/70‑1 (MQ=255)

                                                                   ggatggatGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCa                                                                                                                                >  1:720899/1‑70 (MQ=255)

                                                                      tggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCATATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGAt                                                        >  1:433283/1‑139 (MQ=255)

                                                                                 tGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                    <  1:191248/100‑1 (MQ=255)

                                                                                 tGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                    >  2:191248/1‑100 (MQ=255)

                                                                                 tGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACaa                                             >  1:114391/1‑139 (MQ=255)

                                                                                  gAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                 <  1:366406/118‑1 (MQ=255)

                                                                                  gAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                 >  2:366406/1‑118 (MQ=255)

                                                                                          tctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCccgccg                                    >  2:1355358/1‑139 (MQ=255)

                                                                                           ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCaaa                                                                                                                              >  2:306496/1‑48 (MQ=255)

                                                                                           ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCaaa                                                                                                                              <  1:306496/48‑1 (MQ=255)

                                                                                           ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGa                                   <  2:456741/139‑1 (MQ=255)

                                                                                             gcgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTg                                                          <  2:647406/114‑1 (MQ=255)

                                                                                             gcgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTg                                                          >  1:647406/1‑114 (MQ=255)

                                                                                              cgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                    >  2:1187227/1‑87 (MQ=255)

                                                                                              cgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                    <  1:1187227/87‑1 (MQ=255)

                                                                                                 aCCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAt                             >  1:550544/1‑139 (MQ=255)

                                                                                                      cGGTAAAAAAACAGATGCCTATACCATATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCgtg                        <  2:433283/139‑1 (MQ=255)

                                                                                                           aaaaaaCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGAc                   >  2:1227746/1‑139 (MQ=255)

                                                                                                            aaaaaCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGAcc                  <  2:550544/139‑1 (MQ=255)

                                                                                                             aaaaCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCa                 >  2:1274993/1‑139 (MQ=255)

                                                                                                                aCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCg              <  2:114391/139‑1 (MQ=255)

                                                                                                                  aGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCggg            <  2:10717/139‑1 (MQ=255)

                                                                                                                    aTGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCa          <  2:1198920/139‑1 (MQ=255)

                                                                                                                         tataCCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGa                                                         <  1:960588/87‑1 (MQ=255)

                                                                                                                         tataCCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGa                                                         >  2:960588/1‑87 (MQ=255)

                                                                                                                            aCCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTtct  <  1:1227746/139‑1 (MQ=255)

                                                                                                                                tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCg              >  1:674413/1‑123 (MQ=255)

                                                                                                                                tATTTAGCAAAATGTTAAAAAGAGGACAACCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCg              <  2:674413/123‑1 (MQ=255)

AAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCT  >  NC_000913/3277594‑3277856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A