Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,923,070	G→A	A204V (GCA→GTA)	rsmG ←	16S rRNA m(7)G527 methyltransferase, SAM‑dependent, glucose‑inhibited cell‑division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,923,070	0	G	A	100.0%	84.5 / NA	27	A204V (GCA→GTA)	rsmG	16S rRNA m(7)G527 methyltransferase, SAM‑dependent, glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/15); total (12/15)

GTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAG  >  NC_000913/3922937‑3923191

gTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTa                                                                                                                      <  2:772070/139‑1 (MQ=255)

            aTGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTANNNCAGTANAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGa                                                                                                          <  2:43563/139‑1 (MQ=255)

              gCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAACTTTATTTACTTTAATCACCACCAGATg                                                                                                        >  1:579432/1‑139 (MQ=255)

                                 gTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCt                                                                                     <  1:52617/139‑1 (MQ=255)

                                   tAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTgg                                                                                   >  1:355749/1‑139 (MQ=255)

                                               gTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGttt                                                                       >  2:972845/1‑139 (MQ=255)

                                                ttATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCt                                                                              >  1:1186320/1‑131 (MQ=255)

                                                ttATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCt                                                                              <  2:1186320/131‑1 (MQ=255)

                                                         ttataaaCCTTGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAt                                                             <  1:708912/135‑1 (MQ=255)

                                                            aaaaCCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCg                                                          <  1:386767/139‑1 (MQ=255)

                                                             aaaCCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGa                                                         <  1:1301977/139‑1 (MQ=255)

                                                                   ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGat                                                   >  1:89080/1‑139 (MQ=255)

                                                                   ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGat                                                   >  2:1395274/1‑139 (MQ=255)

                                                                                   cTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAtt                                                            >  1:243396/1‑114 (MQ=255)

                                                                                   cTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAtt                                                            <  2:243396/114‑1 (MQ=255)

                                                                                            cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCg                                                          <  2:816879/107‑1 (MQ=255)

                                                                                            cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGAc                                                        <  1:469881/109‑1 (MQ=255)

                                                                                            cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGAc                                                        >  2:469881/1‑109 (MQ=255)

                                                                                            cAATTTTTTATGATTTTTCTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCg                                                          >  1:816879/1‑107 (MQ=255)

                                                                                                                    aaaaTTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAg  <  1:793794/139‑1 (MQ=255)

                                                                                                                    aaaaTTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAg  <  2:89080/139‑1 (MQ=255)

                                                                                                                                tATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCgg                                          >  2:675352/1‑87 (MQ=255)

                                                                                                                                tATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCgg                                          <  1:675352/87‑1 (MQ=255)

                                                                                                                                tATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAg  >  2:546502/1‑127 (MQ=255)

                                                                                                                                tATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAg  <  1:546502/127‑1 (MQ=255)

                                                                                                                                 aTTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCg                                                          >  2:1187317/1‑70 (MQ=255)

                                                                                                                                 aTTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCg                                                          <  1:1187317/70‑1 (MQ=255)

GTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAG  >  NC_000913/3922937‑3923191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A