Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,923,070	G→A	A204V (GCA→GTA)	rsmG ←	16S rRNA m(7)G527 methyltransferase, SAM‑dependent, glucose‑inhibited cell‑division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,923,070	0	G	A	100.0%	149.1 / NA	46	A204V (GCA→GTA)	rsmG	16S rRNA m(7)G527 methyltransferase, SAM‑dependent, glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/23); total (23/23)

ACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCA  >  NC_000913/3922935‑3923190

aCGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACtt                                                                                                                       <  2:800019/139‑1 (MQ=255)

    tATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAAt                                                                                                                   <  1:744477/139‑1 (MQ=255)

                gCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACtt                                                                                                                       >  1:1001765/1‑123 (MQ=255)

                gCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACtt                                                                                                                       <  2:1001765/123‑1 (MQ=255)

                           aaCGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCAt                                                                                            >  2:511483/1‑139 (MQ=255)

                                     tAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATcac                                                                                                                <  1:1234444/109‑1 (MQ=255)

                                     tAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATcac                                                                                                                >  2:1234444/1‑109 (MQ=255)

                                              ccAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAg                                                                         >  1:1533336/1‑139 (MQ=255)

                                                          gTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGa                                                             >  2:1270568/1‑139 (MQ=255)

                                                           tacaaaaCCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTCTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTgg                                                                                  <  1:157279/114‑1 (MQ=255)

                                                           tAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAt                                                            <  1:1270568/139‑1 (MQ=255)

                                                           tAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTAAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTgg                                                                                  >  2:157279/1‑117 (MQ=255)

                                                                   cTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTg                                                    >  1:322607/1‑139 (MQ=255)

                                                                     ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCt                                                                             >  2:852915/1‑112 (MQ=255)

                                                                     ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCt                                                                             <  1:852915/112‑1 (MQ=255)

                                                                     ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAt                                                            <  1:707472/129‑1 (MQ=255)

                                                                     ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAt                                                            >  2:707472/1‑129 (MQ=255)

                                                                     ggTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGat                                                  >  1:1558084/1‑139 (MQ=255)

                                                                               aaCAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAt                                                            <  2:896301/119‑1 (MQ=255)

                                                                               aaCAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGAt                                                            >  1:896301/1‑119 (MQ=255)

                                                                                    tCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                                <  1:330966/78‑1 (MQ=255)

                                                                                    tCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                                >  2:330966/1‑78 (MQ=255)

                                                                                    tCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGcaaca                                   >  1:1579248/1‑139 (MQ=255)

                                                                                     cTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCa                                                                                         <  1:1692994/84‑1 (MQ=255)

                                                                                     cTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCa                                                                                         >  2:1692994/1‑84 (MQ=255)

                                                                                      tAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACaaa                                 <  1:511483/139‑1 (MQ=255)

                                                                                      tAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACaaa                                 <  2:1533336/139‑1 (MQ=255)

                                                                                       aaCCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                                <  1:1494687/75‑1 (MQ=255)

                                                                                       aaCCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                                >  2:1494687/1‑75 (MQ=255)

                                                                                       aaCCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAg                                <  1:1486622/139‑1 (MQ=255)

                                                                                            gTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCt                                                                             <  1:1065201/89‑1 (MQ=255)

                                                                                            gTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCt                                                                             >  2:1065201/1‑89 (MQ=255)

                                                                                              cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGttt                                                                      <  1:959696/94‑1 (MQ=255)

                                                                                              cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGttt                                                                      >  2:959696/1‑94 (MQ=255)

                                                                                              cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCa                                                                 >  1:996766/1‑99 (MQ=255)

                                                                                              cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCa                                                                 <  2:996766/99‑1 (MQ=255)

                                                                                              cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTc                         >  1:500355/1‑139 (MQ=255)

                                                                                              cAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTc                         >  2:889358/1‑139 (MQ=255)

                                                                                               aaTTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                                >  2:651544/1‑67 (MQ=255)

                                                                                               aaTTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCg                                                                                                <  1:651544/67‑1 (MQ=255)

                                                                                                           tttttttGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCAtt            <  2:1558084/139‑1 (MQ=255)

                                                                                                             tttttGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTg          <  2:1579248/139‑1 (MQ=255)

                                                                                                              ttttGATAAAAATTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACATGATATTCTTCGGGCAACAAAGCGATTTCATATTCCGGCATTTGc         >  2:948333/1‑139 (MQ=255)

                                                                                                                     aaaaaTTAAATTTTATTTACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCa  <  2:500355/139‑1 (MQ=255)

                                                                                                                                    tttACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGAc                                                       <  2:1097506/71‑1 (MQ=255)

                                                                                                                                    tttACTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGAc                                                       >  1:1097506/1‑71 (MQ=255)

ACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCA  >  NC_000913/3922935‑3923190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A