Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I2 R1
|
766 |
64.2 |
3232166 |
90.9% |
2938038 |
104.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,083,620 |
G→A |
R60C (CGT→TGT) |
speB ← |
agmatinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,083,620 | 0 | G | A | 100.0%
| 212.8
/ NA
| 65 | R60C (CGT→TGT) | speB | agmatinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (36/29); total (36/29) |
CTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAGGCA > NC_000913/3083493‑3083753
|
cTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCg < 1:228767/139‑1 (MQ=255)
gggCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCg > 2:1266944/1‑139 (MQ=255)
ggCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGa > 1:1583986/1‑139 (MQ=255)
cATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGAt > 1:898139/1‑118 (MQ=255)
cATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGAt < 2:898139/118‑1 (MQ=255)
tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGa < 1:1211858/135‑1 (MQ=255)
tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGa > 2:1211858/1‑135 (MQ=255)
cAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGc < 2:404434/139‑1 (MQ=255)
aGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACg > 1:572907/1‑139 (MQ=255)
atatACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCa > 2:128188/1‑139 (MQ=255)
cAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGt > 2:1605293/1‑139 (MQ=255)
gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGa > 2:235650/1‑107 (MQ=255)
gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGa < 1:235650/107‑1 (MQ=255)
gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAg < 2:913860/135‑1 (MQ=255)
gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAg > 1:913860/1‑135 (MQ=255)
gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGc > 1:555192/1‑139 (MQ=255)
aTCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGcc > 1:1413812/1‑139 (MQ=255)
aTCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGcc > 1:311370/1‑139 (MQ=255)
aTCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGcc > 2:1292284/1‑139 (MQ=255)
cgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGa < 2:392476/101‑1 (MQ=255)
cgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGa > 1:392476/1‑101 (MQ=255)
cgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATc > 1:167462/1‑139 (MQ=255)
cgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATc > 2:1283298/1‑139 (MQ=255)
cgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATc > 1:1450797/1‑139 (MQ=255)
cAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGc > 2:1488397/1‑116 (MQ=255)
cAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGc < 1:1488397/116‑1 (MQ=255)
tCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACg > 2:1035136/1‑139 (MQ=255)
acgTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGc < 2:366373/139‑1 (MQ=255)
acgTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGc < 1:965172/139‑1 (MQ=255)
acgTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGc < 2:555192/139‑1 (MQ=255)
tCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGt > 2:396929/1‑139 (MQ=255)
cACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCa < 1:1035136/139‑1 (MQ=255)
cACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCa < 1:1292284/139‑1 (MQ=255)
gTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTg > 1:1361633/1‑75 (MQ=255)
gTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTg < 2:1361633/75‑1 (MQ=255)
gTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCa < 1:735341/139‑1 (MQ=255)
gTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCa > 1:648320/1‑139 (MQ=255)
gTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCa < 1:467252/139‑1 (MQ=255)
aaaTTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGt < 1:133908/130‑1 (MQ=255)
aaaTTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGt > 2:133908/1‑130 (MQ=255)
aC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGGCCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATa < 1:1283298/139‑1 (MQ=255)
ggAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTg > 1:1155665/1‑51 (MQ=255)
ggAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTg < 2:1155665/51‑1 (MQ=255)
ggTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGaa < 2:572907/139‑1 (MQ=255)
gTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATa < 2:991678/129‑1 (MQ=255)
gTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATa > 1:991678/1‑129 (MQ=255)
gtgtTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCa > 2:1229146/1‑104 (MQ=255)
gtgtTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCa < 1:1229146/104‑1 (MQ=255)
gtgtTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTc > 2:205722/1‑139 (MQ=255)
aGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTa > 1:1394230/1‑139 (MQ=255)
aGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTa > 1:627453/1‑139 (MQ=255)
aTTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGc > 1:65240/1‑38 (MQ=38)
aTTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGc < 2:65240/38‑1 (MQ=38)
aTTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTaaa > 1:799715/1‑139 (MQ=255)
aTTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTaaa > 1:440126/1‑139 (MQ=255)
ttCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGAACAGAAGTGGCCATATAGGACGGCACGCCAGTAAGCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGGTCATCGGCAGGCGTaaaa > 1:996794/1‑139 (MQ=255)
tcgAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGcc < 2:539562/59‑1 (MQ=255)
tcgAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGcc > 1:539562/1‑59 (MQ=255)
tcgAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGaa > 1:1487639/1‑67 (MQ=255)
tcgAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGaa < 2:1487639/67‑1 (MQ=255)
tcgAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAAcc < 2:440126/139‑1 (MQ=255)
aaCCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAg < 2:1007050/139‑1 (MQ=255)
aaCCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAg > 1:1007050/1‑139 (MQ=255)
aCCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAgg < 1:483287/139‑1 (MQ=255)
cTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAGGCa < 1:1266944/139‑1 (MQ=255)
|
CTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCAC‑GGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAGGCA > NC_000913/3083493‑3083753
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
