Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,248,939	T→C	intergenic (+117/‑30)	mzrA → / → yqjC	modulator of EnvZ/OmpR regulon/DUF1090 family putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,248,939	0	T	C	98.7%	281.3 / NA	79	intergenic (+117/‑30)	mzrA/yqjC	modulator of EnvZ/OmpR regulon/DUF1090 family putative periplasmic protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (38/40); total (39/40)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAATAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAGGAGAT  >  NC_000913/3248809‑3249072

aTCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGa                                                                                                                               >  1:693507/1‑139 (MQ=255)

  cGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                             <  2:1205239/139‑1 (MQ=255)

      tCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATcaca                                                                                                                         >  2:1476541/1‑139 (MQ=255)

               ttCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGt                                                                                                                <  1:1556421/139‑1 (MQ=255)

                 cctattATCCGAAACATATCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                              <  1:57515/135‑1 (MQ=255)

                 ccTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                              <  2:693507/139‑1 (MQ=255)

                        tCCGAAACATATCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGT‑GTTAACAACTCCCCCTCCTTGGCCTGACAATCCTTTCTTTTTAACAAGGCCGAATTACCATGGAAGGGTCAaaaaata                                                                                                      >  2:867839/1‑136 (MQ=255)

                         ccGAAACATATCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGt                                                                                                                                   >  1:304507/1‑110 (MQ=255)

                         ccGAAACATATCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGt                                                                                                                                   <  2:304507/110‑1 (MQ=255)

                          cGAAACATATCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAATAGGTCCGAATCACAATGGa                                                                                                                    >  2:1033601/1‑124 (MQ=255)

                          cGAAACATATCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                                    <  1:1033601/124‑1 (MQ=255)

                                 tatCACTCTCTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGc                                                                                              <  1:979582/139‑1 (MQ=255)

                                  atCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCa                                                                                             >  1:425432/1‑139 (MQ=255)

                                   tcacttacCTTTGTTATTTTCCCGTTAATTGTTTTTGATTATTGA‑GCGGGTGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCAt                                                                                            <  1:867839/139‑1 (MQ=255)

                                         actTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCttt                                                                                      >  1:854999/1‑139 (MQ=255)

                                         actTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCttt                                                                                      >  1:778616/1‑139 (MQ=255)

                                           tttGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAg                                                                                    <  2:858939/139‑1 (MQ=255)

                                           tttGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAg                                                                                    >  1:1135438/1‑139 (MQ=255)

                                              ggTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTg                                                                                 >  1:347276/1‑139 (MQ=255)

                                                tGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGtt                                                                               <  2:1135438/139‑1 (MQ=255)

                                                         cG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                            <  1:1452062/101‑1 (MQ=255)

                                                         cG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                            >  2:1452062/1‑101 (MQ=255)

                                                                  tCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                                    <  1:1439823/85‑1 (MQ=255)

                                                                  tCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                                    >  2:1439823/1‑85 (MQ=255)

                                                                  acTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTCTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTGTCTCTCTTTGCTCTTAg                                                              >  2:991836/2‑139 (MQ=255)

                                                                      tGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGttt                                                                              >  2:421735/1‑119 (MQ=255)

                                                                      tGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGttt                                                                              <  1:421735/119‑1 (MQ=255)

                                                                         ttAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGt                                                       <  2:504947/139‑1 (MQ=255)

                                                                           aaTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAg                                                     <  1:1476541/139‑1 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGaa                                                                                                                              <  2:721094/65‑1 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGaa                                                                                                                              >  1:721094/1‑65 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                            >  1:1337959/1‑83 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                            <  2:1337959/83‑1 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtc                                                                             >  1:655356/1‑114 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtc                                                                             <  2:655356/114‑1 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGt                                                    >  1:1175841/1‑139 (MQ=255)

                                                                             tGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                            >  1:1564808/1‑82 (MQ=255)

                                                                             tGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                            <  2:1564808/82‑1 (MQ=255)

                                                                                 gCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATg                                                                                                       <  2:1393691/83‑1 (MQ=255)

                                                                                 gCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATg                                                                                                       >  1:1393691/1‑83 (MQ=255)

                                                                                 gCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGc                                               >  2:1142123/1‑139 (MQ=255)

                                                                                  cGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                              >  2:1531562/1‑75 (MQ=255)

                                                                                  cGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                              <  1:1531562/75‑1 (MQ=255)

                                                                                  cGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGcc                                              >  2:1229458/1‑139 (MQ=255)

                                                                                        ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATTGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                        <  1:991836/139‑1 (MQ=255)

                                                                                        ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                        <  2:1175841/139‑1 (MQ=255)

                                                                                        ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                        <  1:1229458/139‑1 (MQ=255)

                                                                                        ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                        <  2:347276/139‑1 (MQ=255)

                                                                                        ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTACCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                        <  1:1142123/139‑1 (MQ=255)

                                                                                          aaCACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAg                                                                                    <  1:1507624/93‑1 (MQ=255)

                                                                                          aaCACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAg                                                                                    >  2:1507624/1‑93 (MQ=255)

                                                                                                aCCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                            >  2:67547/1‑63 (MQ=255)

                                                                                                aCCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                            <  1:67547/63‑1 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGaa                                                                                                         >  1:347665/1‑65 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGaa                                                                                                         <  2:347665/65‑1 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCtt                                                                >  1:103238/1‑106 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCtt                                                                <  2:103238/106‑1 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAg                               >  1:136260/1‑139 (MQ=255)

                                                                                                 ccGCATCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATg                                                                                                       <  1:567951/67‑1 (MQ=255)

                                                                                                 ccGCATCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATg                                                                                                       >  2:567951/1‑67 (MQ=255)

                                                                                                    cTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGt                                                             <  2:1020566/106‑1 (MQ=255)

                                                                                                    cTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGaa                            >  2:176769/1‑139 (MQ=255)

                                                                                                    cTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGATTTAGCTGTTTCTCTCTTTGCTCTTAGt                                                             >  1:1020566/1‑106 (MQ=255)

                                                                                                      tCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGaaaa                          >  1:763319/1‑139 (MQ=255)

                                                                                                       cGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCTCAATGGAAGGTTCaa                                                                                                            <  1:1078351/56‑1 (MQ=255)

                                                                                                       cGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACACTGGAAGGTTCaa                                                                                                            >  2:1078351/1‑56 (MQ=255)

                                                                                                       cGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                        >  2:719168/1‑124 (MQ=255)

                                                                                                       cGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                        <  1:719168/124‑1 (MQ=255)

                                                                                                         ttGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTg                                                            <  2:530093/102‑1 (MQ=255)

                                                                                                         ttGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTg                                                            >  1:530093/1‑102 (MQ=255)

                                                                                                         ttGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCg                                                         >  1:874854/1‑105 (MQ=255)

                                                                                                         ttGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCg                                                         <  2:874854/105‑1 (MQ=255)

                                                                                                            tCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCa                                             <  1:1511097/114‑1 (MQ=255)

                                                                                                            tCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCa                                             >  2:1511097/1‑114 (MQ=255)

                                                                                                            tCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCa                    <  2:136260/139‑1 (MQ=255)

                                                                                                                     tCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCt           >  2:1321185/1‑139 (MQ=255)

                                                                                                                         tCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAg       <  2:815807/139‑1 (MQ=255)

                                                                                                                            tttGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAGgag    <  1:342145/139‑1 (MQ=255)

                                                                                                                              tGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAGGAGAt  >  2:1484998/1‑139 (MQ=255)

ATCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCG‑TAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAATAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAGGAGAT  >  NC_000913/3248809‑3249072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A