Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,248,939	T→C	intergenic (+117/‑30)	mzrA → / → yqjC	modulator of EnvZ/OmpR regulon/DUF1090 family putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,248,939	0	T	C	100.0%	334.5 / NA	92	intergenic (+117/‑30)	mzrA/yqjC	modulator of EnvZ/OmpR regulon/DUF1090 family putative periplasmic protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (46/46); total (46/46)

CATCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAATAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAGGAGATC  >  NC_000913/3248808‑3249073

cATCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCg                                                                                                                                 <  2:1797283/139‑1 (MQ=255)

 aTCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGa                                                                                                                                >  1:1918964/1‑139 (MQ=255)

 aTCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGa                                                                                                                                >  2:1094525/1‑139 (MQ=255)

   cGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                              <  2:1810117/139‑1 (MQ=255)

      ttCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATcac                                                                                                                           >  1:6615/1‑139 (MQ=255)

            tAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                                     <  2:1102584/139‑1 (MQ=255)

                    tGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                             <  2:438372/139‑1 (MQ=255)

                          ccGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                              <  1:1160185/116‑1 (MQ=255)

                          ccGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                              >  2:1160185/1‑116 (MQ=255)

                                   atCACTCTCTTTGGTGATTTCACCTTAACTGTCTAGGATTAATGAGGCGGTGTTTAACACTACCGCTTCGTTGTCTGGAGAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCa                                                                                              <  1:1510240/139‑1 (MQ=255)

                                   atCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                                     >  2:1778334/1‑116 (MQ=255)

                                   atCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                                     <  1:1778334/116‑1 (MQ=255)

                                        tcactTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCt                                                                                         >  1:1230600/1‑139 (MQ=255)

                                        tcactTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCt                                                                                         >  2:2028732/1‑139 (MQ=255)

                                            tttGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAg                                                                                     >  1:374184/1‑139 (MQ=255)

                                                    tttCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCt                                                                                         <  2:491183/127‑1 (MQ=255)

                                                    tttCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCt                                                                                         >  1:491183/1‑127 (MQ=255)

                                                    tttCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtct                                                                             >  1:1969369/1‑139 (MQ=255)

                                                        aCCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                               >  2:1440492/1‑101 (MQ=255)

                                                        aCCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                               <  1:1440492/101‑1 (MQ=255)

                                                         ccGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCtt                                                                        <  1:2028732/139‑1 (MQ=255)

                                                          cGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGtt                                                                                <  1:1907099/130‑1 (MQ=255)

                                                          cGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGtt                                                                                >  2:1907099/1‑130 (MQ=255)

                                                          cGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCttt                                                                       >  2:1855586/1‑139 (MQ=255)

                                                             aaCTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGct                                                                    >  1:944629/1‑139 (MQ=255)

                                                               cTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGctct                                                                  >  1:643228/1‑139 (MQ=255)

                                                               cTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGctct                                                                  <  2:643228/139‑1 (MQ=255)

                                                                tGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCtt                                                                 <  2:238051/139‑1 (MQ=255)

                                                                  tCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAg                                                                                     <  2:1251816/117‑1 (MQ=255)

                                                                  tCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAg                                                                                     >  1:1251816/1‑117 (MQ=255)

                                                                       gATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                              >  2:864579/1‑71 (MQ=255)

                                                                       gATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                              <  1:864579/71‑1 (MQ=255)

                                                                       gATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCttt                                                                                       <  1:534064/110‑1 (MQ=255)

                                                                       gATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCttt                                                                                       >  2:534064/1‑110 (MQ=255)

                                                                        aTTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCACGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCgg                                                         >  1:1405948/1‑139 (MQ=255)

                                                                        aTTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCgg                                                         <  2:1405948/139‑1 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                               >  1:572568/1‑81 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                               <  2:572568/81‑1 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAGTACCGCATCGCTTTAGATGt                                                                                 <  2:1398279/111‑1 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCttt                                                                                       >  2:1079121/1‑105 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCttt                                                                                       <  1:1079121/105‑1 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGt                                                                                 >  1:1398279/1‑111 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCg                                                          >  1:1205381/1‑134 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCg                                                          <  2:1205381/134‑1 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGt                                                     >  2:200070/1‑139 (MQ=255)

                                                                            aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGt                                                     >  1:449651/1‑139 (MQ=255)

                                                                                 gCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCg                                                                                                                                 <  1:1676315/58‑1 (MQ=255)

                                                                                 gCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCg                                                                                                                                 >  2:1676315/1‑58 (MQ=255)

                                                                                 gCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGaa                                                                                                                               <  1:1612389/60‑1 (MQ=255)

                                                                                 gCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGaa                                                                                                                               >  2:1612389/1‑60 (MQ=255)

                                                                                   ggtggtTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCa                                              >  2:223438/1‑139 (MQ=255)

                                                                                        ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                         <  2:1969369/139‑1 (MQ=255)

                                                                                        ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                         <  2:1918964/139‑1 (MQ=255)

                                                                                         tAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAccc                                        <  1:1855586/139‑1 (MQ=255)

                                                                                               tACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCg                                                          >  1:107952/1‑115 (MQ=255)

                                                                                               tACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCg                                                          <  2:107952/115‑1 (MQ=255)

                                                                                               tACCGCGTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAGCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTc                                  >  1:601416/1‑139 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATc                                                                                                                             >  1:909866/1‑46 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATc                                                                                                                             <  2:909866/46‑1 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                                     >  2:634397/1‑54 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                                     <  1:634397/54‑1 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCtt                                                                                        <  2:1040888/83‑1 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCtt                                                                                        >  1:1040888/1‑83 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGctc                                                                   <  1:1568087/104‑1 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGctc                                                                   >  2:1568087/1‑104 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCg                                                          <  1:1069752/113‑1 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCg                                                          >  2:1069752/1‑113 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAg                                >  2:805650/1‑139 (MQ=255)

                                                                                                 ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAg                                >  1:812757/1‑139 (MQ=255)

                                                                                                     ttCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGaaa                            <  2:449651/139‑1 (MQ=255)

                                                                                                     ttCGTTGTCTGGAAAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGaaa                            <  2:812757/139‑1 (MQ=255)

                                                                                                       cGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCa                                              <  1:1692599/119‑1 (MQ=255)

                                                                                                       cGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCa                                              >  2:1692599/1‑119 (MQ=255)

                                                                                                          tGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAg                       <  1:56793/139‑1 (MQ=255)

                                                                                                            tCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCa                     >  1:1054611/1‑139 (MQ=255)

                                                                                                               ggACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCaaaa                  <  2:1230600/139‑1 (MQ=255)

                                                                                                               ggACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCaaaa                  <  2:1054611/139‑1 (MQ=255)

                                                                                                                 aCAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAata                <  2:374184/139‑1 (MQ=255)

                                                                                                                      cGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCtt                                                                 <  1:1873844/85‑1 (MQ=255)

                                                                                                                      cGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCtt                                                                 >  2:1873844/1‑85 (MQ=255)

                                                                                                                      cGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAGTATCCtt           >  1:202075/1‑139 (MQ=255)

                                                                                                                       gTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTa          <  1:223438/139‑1 (MQ=255)

                                                                                                                        ttCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTa          <  1:1923129/138‑1 (MQ=255)

                                                                                                                        ttCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTa          >  2:1923129/1‑138 (MQ=255)

                                                                                                                         tCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAg        <  2:6615/139‑1 (MQ=255)

                                                                                                                          ccTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCAc                                             <  1:835855/101‑1 (MQ=255)

                                                                                                                          ccTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCAc                                             >  2:835855/1‑101 (MQ=255)

                                                                                                                          ccTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCaaaa                  <  1:789813/128‑1 (MQ=255)

                                                                                                                          ccTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCaaaa                  >  2:789813/1‑128 (MQ=255)

                                                                                                                               gTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCAt                                                                                             <  2:2054154/48‑1 (MQ=255)

                                                                                                                               gTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCAt                                                                                             >  1:2054154/1‑48 (MQ=255)

                                                                                                                               gTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAGGAGATc  >  2:95994/1‑139 (MQ=255)

CATCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAATAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAGGAGATC  >  NC_000913/3248808‑3249073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A