Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,781,688	G→A	158 (TAG→TAA)	trmL →	tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,688	0	G	A	100.0%	147.3 / NA	46	158 (TAG→TAA)	trmL	tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/28); total (18/28)

CTGCCCGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTG  >  NC_000913/3781554‑3781823

ctgcccGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTAc                                                                                                                                     >  2:1413819/1‑139 (MQ=255)

 tgcccGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACt                                                                                                                                    >  1:634131/1‑139 (MQ=255)

   cccGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGt                                                                                                                                  >  2:586807/1‑139 (MQ=255)

     cGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAg                                                                                                                                <  1:856255/139‑1 (MQ=255)

      gCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAgg                                                                                                                               <  2:1253623/139‑1 (MQ=255)

        tGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGcc                                                                                                                             >  1:1394428/1‑139 (MQ=255)

             aaaaaaTTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATa                                                                                                                        <  2:979112/139‑1 (MQ=255)

             aaaaaaTTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATa                                                                                                                        <  2:1394428/139‑1 (MQ=255)

                    tggCATTCCGATGGTGGCGGACAGCCGCAGCATGAATCTGTCCAATGCGATGTCGGTATTGGTGTATGACGCGTTGCGGCATTTGGGCTATCGGGGAGCGGTATTGAGAGATAAAGTACTGTAGGCCGGATAGATCAtt                                                                                                                 <  1:234282/137‑1 (MQ=255)

                             gATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCAt                                                                                                        <  1:1413819/139‑1 (MQ=255)

                                    ccGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                 >  2:1237744/1‑139 (MQ=255)

                                    ccGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                 >  1:876517/1‑139 (MQ=255)

                                         cAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca                                                                                            <  2:475190/139‑1 (MQ=255)

                                           gCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca                                                                                          <  2:634131/139‑1 (MQ=255)

                                           gCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca                                                                                          <  1:1245672/139‑1 (MQ=255)

                                            ccGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca                                                                                            <  1:66820/136‑1 (MQ=255)

                                            ccGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca                                                                                            >  2:66820/1‑136 (MQ=255)

                                            ccGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAg                                                                                         <  2:42324/139‑1 (MQ=255)

                                                 gcaTGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACAt                                                                                    <  1:616027/139‑1 (MQ=255)

                                                            tCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAgg                                                                                                                               <  1:884577/85‑1 (MQ=255)

                                                            tCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAgg                                                                                                                               >  2:884577/1‑85 (MQ=255)

                                                             ccAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGatcccatcc                                                                        <  1:912371/139‑1 (MQ=255)

                                                                    ggTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACt                                                                 <  2:876517/139‑1 (MQ=255)

                                                                           gTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                          <  1:586807/139‑1 (MQ=255)

                                                                                        aaGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                 <  2:1060582/87‑1 (MQ=255)

                                                                                        aaGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                 >  1:1060582/1‑87 (MQ=255)

                                                                                           ccTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTg                                          <  2:680904/139‑1 (MQ=255)

                                                                                            cTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGa                                         <  2:241226/139‑1 (MQ=255)

                                                                                              ggcggcAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca                                                                                            >  2:1193640/1‑86 (MQ=255)

                                                                                              ggcggcAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca                                                                                            <  1:1193640/86‑1 (MQ=255)

                                                                                              ggcggcAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                          <  1:924111/120‑1 (MQ=255)

                                                                                              ggcggcAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                          >  2:924111/1‑120 (MQ=255)

                                                                                                     gTTGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGt                                <  1:1237744/139‑1 (MQ=255)

                                                                                                               ccGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCa                                                                                                  >  2:1146594/1‑63 (MQ=255)

                                                                                                               ccGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCa                                                                                                  <  1:1146594/63‑1 (MQ=255)

                                                                                                                        gTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCa                                                                     <  2:458834/83‑1 (MQ=255)

                                                                                                                        gTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCa                                                                     >  1:458834/1‑83 (MQ=255)

                                                                                                                         tATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                 >  2:910262/1‑54 (MQ=255)

                                                                                                                         tATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                 <  1:910262/54‑1 (MQ=255)

                                                                                                                              agagaTTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTa                                                                                  <  2:63216/64‑1 (MQ=255)

                                                                                                                              agagaTTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTa                                                                                  >  1:63216/1‑64 (MQ=255)

                                                                                                                                  attaattaCTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                 <  1:249883/45‑1 (MQ=255)

                                                                                                                                  attaattaCTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                 >  2:249883/1‑45 (MQ=255)

                                                                                                                                  attaattaCTGTAGGCCGGATAGATGATTACATCGCATCCGACACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCatat                          <  2:658000/116‑1 (MQ=255)

                                                                                                                                  attaattaCTGTAGGCCGGATAGATGATTACATCGCATCCGACACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCatat                          >  1:658000/1‑116 (MQ=255)

                                                                                                                                   ttAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCa                                                               <  1:272479/78‑1 (MQ=255)

                                                                                                                                   ttAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCa                                                               >  2:272479/1‑78 (MQ=255)

                                                                                                                                   ttAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTcc                              >  1:1242069/1‑111 (MQ=255)

                                                                                                                                   ttAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTAt           <  2:79984/130‑1 (MQ=255)

                                                                                                                                   ttAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTg  >  1:53818/1‑139 (MQ=255)

CTGCCCGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTG  >  NC_000913/3781554‑3781823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A