Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I2 R1 760 52.7 2744488 90.2% 2475528 105.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,781,688 G→A *158* (TAG→TAA)  trmL → tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,6880GA100.0% 171.8 / NA 53*158* (TAG→TAA) trmLtRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/31);  total (22/31)

CCTGCCCGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCT  >  NC_000913/3781553‑3781822
                                                                                                                                        |                                                                                                                                      
cctgcccGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGattaatta                                                                                                                                     >  2:165183/1‑139 (MQ=255)
 ctgcccGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGGATCCGGGGGCGGTATTTAGAGATTAATTAc                                                                                                                                    >  2:998006/1‑139 (MQ=255)
   gcccGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTg                                                                                                                                  >  1:1292979/1‑139 (MQ=255)
       gCTGAACACAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAgg                                                                                                                              <  1:846554/139‑1 (MQ=255)
       gCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAgg                                                                                                                              <  1:1165517/139‑1 (MQ=255)
          gAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCg                                                                                                                           <  2:205202/139‑1 (MQ=255)
            aCAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGa                                                                                                                         <  2:410118/139‑1 (MQ=255)
                           tCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCC‑GGAGCGGTATTGAGAGATTAATTACTGTAGGc                                                                                                                             <  2:679002/119‑1 (MQ=255)
                           tCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCC‑GGAGCGGTATTGAGAGATTAATTACTGTAGGc                                                                                                                             >  1:679002/1‑119 (MQ=255)
                                tGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATcc                                                                                                     <  1:1009055/139‑1 (MQ=255)
                                     ccGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCa                                                                                                 <  1:1295660/138‑1 (MQ=255)
                                     ccGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCa                                                                                                 >  2:1295660/1‑138 (MQ=255)
                                     ccGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                >  2:389686/1‑139 (MQ=255)
                                          cAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACGTCGCATCCGGCACGATca                                                                                           <  1:760002/139‑1 (MQ=255)
                                          cAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca                                                                                           <  2:477606/139‑1 (MQ=255)
                                            gCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca                                                                                         <  1:165183/139‑1 (MQ=255)
                                             ccGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGAt                                                                                             <  1:1345412/135‑1 (MQ=255)
                                             ccGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTTGGGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGAt                                                                                             >  2:1345412/1‑135 (MQ=255)
                                              cGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca                                                                                           >  2:1264137/1‑135 (MQ=255)
                                              cGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca                                                                                           <  1:1264137/135‑1 (MQ=255)
                                                             tCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGatcccatc                                                                        >  2:719914/1‑139 (MQ=255)
                                                             tCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGatcccatc                                                                        >  1:867904/1‑139 (MQ=255)
                                                                     ggTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACt                                                                <  1:656030/139‑1 (MQ=255)
                                                                       tgtCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCa                                                              >  1:413904/1‑139 (MQ=255)
                                                                       tgtCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCa                                                              >  2:748763/1‑139 (MQ=255)
                                                                            gTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                         <  2:413904/139‑1 (MQ=255)
                                                                            gTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                         <  2:358546/139‑1 (MQ=255)
                                                                            gTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTAAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                         <  2:867904/139‑1 (MQ=255)
                                                                              aGTGGTGTATGACGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGCACATTAGATCCCATCCCCATACTCAAATGTAt                                                       <  1:813934/139‑1 (MQ=255)
                                                                                tggtgTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                <  1:756564/96‑1 (MQ=255)
                                                                                tggtgTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCAc                                                                                                >  2:756564/1‑96 (MQ=255)
                                                                                     tATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATcccc                                                                     >  2:417765/1‑118 (MQ=255)
                                                                                     tATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATcccc                                                                     <  1:417765/118‑1 (MQ=255)
                                                                                      aTGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATa                                               <  1:389686/139‑1 (MQ=255)
                                                                                          aGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGt                                           >  1:983898/1‑139 (MQ=255)
                                                                                            ccTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca                                                                                           >  2:37061/1‑89 (MQ=255)
                                                                                            ccTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATca                                                                                           <  1:37061/89‑1 (MQ=255)
                                                                                               ggcggcAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCg                                                                                                          >  1:1159711/1‑71 (MQ=255)
                                                                                               ggcggcAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCg                                                                                                          <  2:1159711/71‑1 (MQ=255)
                                                                                               ggcggcAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGAt                                                                                             >  2:953546/1‑84 (MQ=255)
                                                                                               ggcggcAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGAt                                                                                             <  1:953546/84‑1 (MQ=255)
                                                                                                 cggcAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAGATGTATGGTTAATACCGTTGAAATg                                    <  1:719914/139‑1 (MQ=255)
                                                                                                 cggcAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATg                                    <  2:1292979/139‑1 (MQ=255)
                                                                                                   gcAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCt                                  <  1:748763/139‑1 (MQ=255)
                                                                                                     aGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca                                                                                         <  2:157867/82‑1 (MQ=255)
                                                                                                     aGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATcaca                                                                                         >  1:157867/1‑82 (MQ=255)
                                                                                                                tCCGGGAGCGGTATTGAGAGATTAATTACTGTAgg                                                                                                                              <  2:183879/35‑1 (MQ=255)
                                                                                                                tCCGGGAGCGGTATTGAGAGATTAATTACTGTAgg                                                                                                                              >  1:183879/1‑35 (MQ=37)
                                                                                                                        cGGTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGc              >  1:1321659/1‑139 (MQ=255)
                                                                                                                          gTATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCtt            >  2:590967/1‑139 (MQ=255)
                                                                                                                           tATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                         <  2:1064121/93‑1 (MQ=255)
                                                                                                                           tATTGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGt                                                         >  1:1064121/1‑93 (MQ=255)
                                                                                                                              tGAGAGATTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCg        <  1:343724/139‑1 (MQ=255)
                                                                                                                                   gaTTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCa                                                                    >  2:663710/1‑74 (MQ=255)
                                                                                                                                   gaTTAATTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCa                                                                    <  1:663710/74‑1 (MQ=255)
                                                                                                                                    attaattaCTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCg        >  2:1013207/1‑133 (MQ=255)
                                                                                                                                    attaattaCTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCg        <  1:1013207/133‑1 (MQ=255)
                                                                                                                                    attaattaCTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCt  >  1:255499/1‑139 (MQ=255)
                                                                                                                                    attaattaCTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCt  >  2:713898/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CCTGCCCGCTGAACAAAAAATTCGCATTCCGATGGTGCCGGACAGCCGCAGCATGAATCTGTCCAATGCGGTGTCGGTAGTGGTGTATGAAGCCTGGCGGCAGTT‑GGGGTATCCGGGAGCGGTATTGAGAGATTAGTTACTGTAGGCCGGATAGATGATTACATCGCATCCGGCACGATCACAGGACATTAGATCCCATCCCCATACTCAAATGTATGGTTAATACCGTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCT  >  NC_000913/3781553‑3781822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A