Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
770 |
51.4 |
2439994 |
93.8% |
2288714 |
110.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,839,384 |
T→A |
intergenic (‑120/‑140) |
ascG ← / → ascF |
asc operon transcriptional repressor, prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,839,384 | 0 | T | A | 98.0%
| 154.3
/ NA
| 51 | intergenic (‑120/‑140) | ascG/ascF | asc operon transcriptional repressor, prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (29/21); total (29/22) |
| Fisher's exact test for biased strand distribution p-value = 4.31e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAA > NC_000913/2839253‑2839517
|
cATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCActct < 2:387308/139‑1 (MQ=255)
cgtcgtCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGCAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCtt > 2:91094/1‑139 (MQ=255)
gtcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTg > 2:962660/1‑139 (MQ=255)
catcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGCAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTAt < 1:91094/139‑1 (MQ=255)
aCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCa > 1:105249/1‑97 (MQ=255)
aCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCa < 2:105249/97‑1 (MQ=255)
cGCAGTGTACTACACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCa > 2:75208/1‑96 (MQ=255)
cGCAGTGTACTACACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCa < 1:75208/96‑1 (MQ=255)
gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCgg < 1:962660/139‑1 (MQ=255)
gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAAACGTCGGGAAAAATATTCAGGTGACCGTTTTCACAAATATAAAAAATGAACAAATCGCTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCgg < 1:1177489/139‑1 (MQ=255)
aCTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGc < 1:471299/97‑1 (MQ=255)
aCTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGc > 2:471299/1‑97 (MQ=255)
tGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCActct > 2:1167492/1‑90 (MQ=255)
tGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCActct < 1:1167492/90‑1 (MQ=255)
tGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTAt > 2:783266/1‑99 (MQ=255)
tGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTAt < 1:783266/99‑1 (MQ=255)
tttGCCGATTGTCCTTGCACGATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCg > 1:1055297/1‑139 (MQ=255)
tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCAct > 2:383575/1‑81 (MQ=255)
tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCAct < 1:383575/81‑1 (MQ=255)
tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGt < 2:462089/97‑1 (MQ=255)
tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGt > 1:462089/1‑97 (MQ=255)
tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAc < 1:293396/123‑1 (MQ=255)
tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCg > 1:361902/1‑139 (MQ=255)
tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCg > 2:137650/1‑139 (MQ=255)
tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTAATCATGATTTTGTGAAAc > 2:293396/1‑123 (MQ=255)
tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGATACACAAATATAAAAAATGAACAAATCAATCTCTTGCTTATTTAGTGACAACTATTCATGAATTTGTGAAACCGGTTTCTTAATTCCg > 2:518334/1‑139 (MQ=255)
tttGCCGATTGTCCGTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATTATTTTGTGAAACCGGTTTCTTAATTCCg > 1:322760/1‑139 (MQ=255)
cTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGt > 1:311241/1‑84 (MQ=255)
cTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGt < 2:311241/84‑1 (MQ=255)
aTCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTcc > 2:434280/1‑139 (MQ=255)
aTCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTcc > 1:30661/1‑139 (MQ=255)
aTCGGCGGGAAAAATATTCAGGTGACCGGATTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTcc > 2:1205161/1‑139 (MQ=255)
tCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg < 2:30661/139‑1 (MQ=255)
ggAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGt < 2:159883/139‑1 (MQ=255)
atTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGAt < 1:434280/139‑1 (MQ=255)
aGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATaaca > 1:1042399/1‑139 (MQ=255)
gTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATaacaac > 2:528861/1‑139 (MQ=255)
tGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATaacaac > 2:170973/1‑138 (MQ=255)
tGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATaacaac < 1:170973/138‑1 (MQ=255)
ggTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATc < 1:137650/139‑1 (MQ=255)
tttCACAAATATAAAAAATGAACAATTCACTCGCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTa < 1:518334/139‑1 (MQ=255)
tCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTAcc > 1:1080552/1‑139 (MQ=255)
tCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTAcc > 2:894525/1‑139 (MQ=255)
aaTATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGACACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACt < 1:1205161/139‑1 (MQ=255)
ataAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGttt < 2:159604/82‑1 (MQ=255)
ataAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGttt > 1:159604/1‑82 (MQ=255)
ataAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGAt < 2:488560/115‑1 (MQ=255)
ataAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGAt > 1:488560/1‑115 (MQ=255)
gAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATa > 2:1037157/1‑139 (MQ=255)
aaCAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATaa > 2:104262/1‑139 (MQ=255)
aaCAAATCACTCTCTTGCTTATTTAGTGACAAATATTCATGATTTTGTGAAACCGGATTCTTAATTCGGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAAGAACTAAATCTACACTACTAATAAAAGGATaa > 1:57009/1‑139 (MQ=255)
|
CATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAA > NC_000913/2839253‑2839517
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
