Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,839,384	T→A	intergenic (‑120/‑140)	ascG ← / → ascF	asc operon transcriptional repressor, prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,839,384	0	T	A	100.0%	94.9 / NA	32	intergenic (‑120/‑140)	ascG/ascF	asc operon transcriptional repressor, prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (16/16); total (16/16)

CCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGAT  >  NC_000913/2839249‑2839515

ccAGTATCTTCGTCATCGTTGTCATCCCTTTACACGCAATCAACGCAGTGTACTGCATCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCa                                                                                                                                  <  1:388958/139‑1 (MQ=255)

ccAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCa                                                                                                                                  <  1:242039/139‑1 (MQ=255)

   gCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCActc                                                                                                                               <  2:567131/139‑1 (MQ=255)

        gtcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTg                                                                                                                          >  2:512305/1‑139 (MQ=255)

         tcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTAAGGTGACCGGTTTCACAAATATACAAAATGAACAAATCACTCTCTTGc                                                                                                                         >  2:388958/1‑139 (MQ=255)

                             ttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTc                                                                                                     <  1:156259/139‑1 (MQ=255)

                             ttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTc                                                                                                     <  1:512305/139‑1 (MQ=255)

                                    cAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACt                                                                                                         >  1:134431/1‑128 (MQ=255)

                                    cAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACt                                                                                                         <  2:134431/128‑1 (MQ=255)

                                     aaTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCActct                                                                                                                              <  1:43397/106‑1 (MQ=255)

                                     aaTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCActct                                                                                                                              >  2:43397/1‑106 (MQ=255)

                                           cGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGaaa                                                                                       >  2:66299/1‑139 (MQ=255)

                                                       cACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCAct                                                                                                                                >  1:15667/1‑86 (MQ=255)

                                                       cACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCAct                                                                                                                                <  2:15667/86‑1 (MQ=255)

                                                       cACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCgg                                                                                   <  2:285934/131‑1 (MQ=255)

                                                       cACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCgg                                                                                   >  1:285934/1‑131 (MQ=255)

                                                            tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCAct                                                                                                                                >  2:137319/1‑81 (MQ=255)

                                                            tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCAct                                                                                                                                <  1:137319/81‑1 (MQ=255)

                                                            tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTc                                                                                                     >  1:122389/1‑108 (MQ=255)

                                                            tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTc                                                                                                     <  2:122389/108‑1 (MQ=255)

                                                            tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTATCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCg                                                                      >  1:643940/1‑139 (MQ=255)

                                                               gCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGttt                                                                   <  2:277028/139‑1 (MQ=255)

                                                                             cacaATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCgg                                                                                   >  1:191150/1‑109 (MQ=255)

                                                                             cacaATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCgg                                                                                   <  2:191150/109‑1 (MQ=255)

                                                                                  tCGGCGGGAAAAATATTCAGGTGACTGGTTTCACAAATATAAAAAATGAACAAATCACTCACTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg                                                >  1:288538/1‑139 (MQ=255)

                                                                                      cggGAAAAATACTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTAt                                                                                                                     >  1:13038/1‑66 (MQ=255)

                                                                                      cggGAAAAATACTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTAt                                                                                                                     <  2:13038/66‑1 (MQ=255)

                                                                                                    aGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATaaca                              <  1:66299/139‑1 (MQ=255)

                                                                                                                        ataAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGAt          >  1:874831/1‑139 (MQ=255)

                                                                                                                        ataAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGAt          >  2:19396/1‑139 (MQ=255)

                                                                                                                         taAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATa         <  2:874831/139‑1 (MQ=255)

                                                                                                                                tGAACAAATCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGAt  >  1:679282/1‑139 (MQ=255)

CCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGAT  >  NC_000913/2839249‑2839515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A