Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I1 R1 770 51.4 2439994 93.8% 2288714 110.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,871,264 G→A P14L (CCG→CTG)  truD ← tRNA(Glu) pseudouridine(13) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,871,2640GA100.0% 128.5 / NA 40P14L (CCG→CTG) truDtRNA(Glu) pseudouridine(13) synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/21);  total (19/21)

GCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTC  >  NC_000913/2871129‑2871378
                                                                                                                                       |                                                                                                                  
gCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGtt                                                                                                                 <  2:1170163/139‑1 (MQ=255)
         cGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGa                                                                                                        <  1:1021042/139‑1 (MQ=255)
            gTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGt                                                                                                     >  1:1148494/1‑139 (MQ=255)
            gTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGt                                                                                                     <  2:1148494/139‑1 (MQ=255)
                                     tAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCAt                                                                            >  2:627154/1‑139 (MQ=255)
                                         cAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTttg                                                                        <  2:431629/139‑1 (MQ=255)
                                           gAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTa                                                                                                    >  2:855963/1‑109 (MQ=255)
                                           gAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTa                                                                                                    <  1:855963/109‑1 (MQ=255)
                                                      ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAg                                                                                                  >  1:267407/1‑100 (MQ=255)
                                                      ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCaaa                                                                                     >  1:276589/1‑113 (MQ=255)
                                                      ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCaaa                                                                                     <  2:276589/113‑1 (MQ=255)
                                                      ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAg                                                           >  1:1013524/1‑139 (MQ=255)
                                                      ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCAGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAg                                                                                                  <  2:267407/100‑1 (MQ=255)
                                                          cACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGa                                                                                                        >  1:1151612/1‑90 (MQ=255)
                                                          cACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGa                                                                                                        <  2:1151612/90‑1 (MQ=255)
                                                            ccATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAgcgc                                                     <  2:1013524/139‑1 (MQ=255)
                                                               tCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTaa                                                                                                   <  1:815363/90‑1 (MQ=255)
                                                               tCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGAATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTaa                                                                                                   >  2:815363/1‑90 (MQ=255)
                                                                 aGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAg                                                        >  2:440163/1‑131 (MQ=255)
                                                                 aGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAg                                                        <  1:440163/131‑1 (MQ=255)
                                                                 aGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCg                                                >  1:368566/1‑139 (MQ=255)
                                                                    cTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTc                                                                                  >  2:22484/1‑102 (MQ=255)
                                                                    cTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTc                                                                                  <  1:22484/102‑1 (MQ=255)
                                                                     tCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTc                                            <  2:368566/139‑1 (MQ=255)
                                                                         aGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAg                                                                                                  <  1:563083/81‑1 (MQ=255)
                                                                         aGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAg                                                                                                  >  2:563083/1‑81 (MQ=255)
                                                                           cccAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGc                                      <  1:627154/139‑1 (MQ=255)
                                                                           cccAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGc                                      <  2:549393/139‑1 (MQ=255)
                                                                                 tCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATccc                                <  2:363434/139‑1 (MQ=255)
                                                                                           ccacAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGt                      >  2:190826/1‑139 (MQ=255)
                                                                                                     ttCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAg                                                                                                  <  2:622054/53‑1 (MQ=255)
                                                                                                     ttCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAg                                                                                                  >  1:622054/1‑53 (MQ=255)
                                                                                                       ccGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATccc          >  2:1024655/1‑139 (MQ=255)
                                                                                                             tGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTttg                                                                        <  2:1144128/71‑1 (MQ=255)
                                                                                                             tGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTttg                                                                        >  1:1144128/1‑71 (MQ=255)
                                                                                                             tGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGt                      <  2:1188855/121‑1 (MQ=255)
                                                                                                             tGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGt                      >  1:1188855/1‑121 (MQ=255)
                                                                                                             tGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGtttt    >  1:507589/1‑139 (MQ=255)
                                                                                                               gCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTc  <  1:190826/139‑1 (MQ=255)
                                                                                                               gCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGATGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGttcttc  >  2:446963/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                  
GCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTC  >  NC_000913/2871129‑2871378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A