Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,871,264	G→A	P14L (CCG→CTG)	truD ←	tRNA(Glu) pseudouridine(13) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,871,264	0	G	A	100.0%	275.8 / NA	83	P14L (CCG→CTG)	truD	tRNA(Glu) pseudouridine(13) synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (42/41); total (42/41)

CACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTC  >  NC_000913/2871131‑2871394

cacaAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTa                                                                                                                               <  2:1061241/139‑1 (MQ=255)

  caAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTAcc                                                                                                                             <  1:1691924/139‑1 (MQ=255)

   aaaaCGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCg                                                                                                                            <  1:490036/139‑1 (MQ=255)

       cGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGa                                                                                                                        <  2:937439/139‑1 (MQ=255)

        gggTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAg                                                                                                                       <  1:64588/139‑1 (MQ=255)

          gTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGt                                                                                                                     >  2:368896/1‑139 (MQ=255)

            aTTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTaa                                                                                                                   <  1:1265535/139‑1 (MQ=255)

               gCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTg                                                                                                                >  2:1055045/1‑139 (MQ=255)

                cAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTga                                                                                                               <  1:1055045/139‑1 (MQ=255)

                  gCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTgaga                                                                                                             <  1:368896/139‑1 (MQ=255)

                     gTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTa                                                                                                          >  1:829685/1‑139 (MQ=255)

                                ttCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAAt                                                                                               >  2:1308540/1‑139 (MQ=255)

                                 tCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCg                                                                                                                                    <  1:704711/101‑1 (MQ=255)

                                 tCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCg                                                                                                                                    >  2:704711/1‑101 (MQ=255)

                                 tCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATc                                                                                              >  2:1742551/1‑139 (MQ=255)

                                   tAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCAt                                                                                            >  2:186807/1‑139 (MQ=255)

                                       cAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTttg                                                                                        <  1:186807/139‑1 (MQ=255)

                                       cAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTttg                                                                                        <  2:829685/139‑1 (MQ=255)

                                          aaTATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGt                                                                                                                     <  2:724593/107‑1 (MQ=255)

                                          aaTATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGt                                                                                                                     >  1:724593/1‑107 (MQ=255)

                                               gCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAg                                                                                                                       <  2:1350645/100‑1 (MQ=255)

                                               gCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCAGTGCCTTGCAGTTTACCGTGGAg                                                                                                                       >  1:1350645/1‑100 (MQ=255)

                                                 tCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAAt                                                                              >  1:1589722/1‑139 (MQ=255)

                                                   aCCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGa                                                                            >  2:1765719/1‑139 (MQ=255)

                                                    ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCaaa                                                                                                     >  1:19501/1‑113 (MQ=255)

                                                    ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCaaa                                                                                                     <  2:19501/113‑1 (MQ=255)

                                                    ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAg                                                                           >  2:1602524/1‑139 (MQ=255)

                                                    ccTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAg                                                                           >  2:1525592/1‑139 (MQ=255)

                                                         aCCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAg                                                                                                                       <  1:202637/90‑1 (MQ=255)

                                                         aCCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAg                                                                                                                       >  2:202637/1‑90 (MQ=255)

                                                              cAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCAcc                                                                 <  1:1525592/139‑1 (MQ=255)

                                                              cAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCAcc                                                                 <  1:1480562/139‑1 (MQ=255)

                                                              cAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCAcc                                                                 <  1:1765719/139‑1 (MQ=255)

                                                               aGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCg                                                                >  2:912093/1‑139 (MQ=255)

                                                               aGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCg                                                                >  2:785040/1‑139 (MQ=255)

                                                               aGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCg                                                                >  2:729795/1‑139 (MQ=255)

                                                               aGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCg                                                                >  1:1763923/1‑139 (MQ=255)

                                                                 gCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCg                                                                                                                            >  1:1868201/1‑77 (MQ=255)

                                                                 gCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCg                                                                                                                            <  2:1868201/77‑1 (MQ=255)

                                                                 gCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACt                                                                                                   <  1:296691/102‑1 (MQ=255)

                                                                 gCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACt                                                                                                   >  2:296691/1‑102 (MQ=255)

                                                                  cTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCtt                                                             >  2:1660890/1‑139 (MQ=255)

                                                                    cAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTca                                                           <  1:1660890/139‑1 (MQ=255)

                                                                    cAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTca                                                           <  1:912093/139‑1 (MQ=255)

                                                                       aGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAg                                                                                                                       >  1:1733115/1‑76 (MQ=255)

                                                                       aGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAg                                                                                                                       <  2:1733115/76‑1 (MQ=255)

                                                                           cAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTaa                                                                                                                   <  1:1763995/76‑1 (MQ=255)

                                                                           cAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTaa                                                                                                                   >  2:1763995/1‑76 (MQ=255)

                                                                           cAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCaa                                                    >  1:221035/1‑139 (MQ=255)

                                                                             aaTCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGt                                                                                                                           >  2:1285671/1‑66 (MQ=255)

                                                                             aaTCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGt                                                                                                                           <  1:1285671/66‑1 (MQ=255)

                                                                             aaTCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATc                                                  <  1:785040/139‑1 (MQ=255)

                                                                                    caccaccacAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTccc                                           <  1:729795/139‑1 (MQ=255)

                                                                                     accaccacAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTcccc                                          <  2:1763923/139‑1 (MQ=255)

                                                                                        accacAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACg                                       >  2:1844268/1‑139 (MQ=255)

                                                                                         ccacAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCa                                                                                                       <  1:343684/74‑1 (MQ=255)

                                                                                         ccacAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCa                                                                                                       >  2:343684/1‑74 (MQ=255)

                                                                                          cacaAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACg                                       >  1:282908/1‑137 (MQ=255)

                                                                                          cacaAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACg                                       >  1:212925/1‑137 (MQ=255)

                                                                                          cacaAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACg                                       <  2:212925/137‑1 (MQ=255)

                                                                                          cacaAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACg                                       <  2:282908/137‑1 (MQ=255)

                                                                                                    tCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGtt                                                                                                                                 <  1:1283825/37‑1 (MQ=255)

                                                                                                    tCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGtt                                                                                                                                 >  2:1283825/1‑37 (MQ=255)

                                                                                                    tCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACt                                                                                                   <  2:1442778/67‑1 (MQ=255)

                                                                                                    tCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACt                                                                                                   >  1:1442778/1‑67 (MQ=255)

                                                                                                    tCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTttgttg                                                                                     <  1:286769/81‑1 (MQ=255)

                                                                                                    tCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTttgttg                                                                                     >  2:286769/1‑81 (MQ=255)

                                                                                                    tCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTccc                                           <  2:1359000/123‑1 (MQ=255)

                                                                                                    tCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTccc                                           >  1:1359000/1‑123 (MQ=255)

                                                                                                    tCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATcc                           >  2:1710501/1‑139 (MQ=255)

                                                                                                    tCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATcc                           >  2:83798/1‑139 (MQ=255)

                                                                                                    tCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATcc                           >  2:975224/1‑139 (MQ=255)

                                                                                                         aTTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCaa                                                    <  2:768017/109‑1 (MQ=255)

                                                                                                         aTTGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCaa                                                    >  1:768017/1‑109 (MQ=255)

                                                                                                          ttGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGttt                     <  2:221035/139‑1 (MQ=255)

                                                                                                           tGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTccc                                           >  1:1864910/1‑116 (MQ=255)

                                                                                                           tGGCTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTccc                                           <  2:1864910/116‑1 (MQ=255)

                                                                                                              cTTTCAGCAGCCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTcc                 <  1:1742551/139‑1 (MQ=255)

                                                                                                                       gcCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAAt                                                                                               <  1:865176/52‑1 (MQ=255)

                                                                                                                       gcCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAAt                                                                                               >  2:865176/1‑52 (MQ=255)

                                                                                                                       gcCCGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTg        >  2:213755/1‑139 (MQ=255)

                                                                                                                        cccGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTTAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCa                                                     >  2:744096/1‑93 (MQ=255)

                                                                                                                        cccGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCa                                                     <  1:744096/93‑1 (MQ=255)

                                                                                                                          cGGTGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCt     <  1:1290158/139‑1 (MQ=255)

                                                                                                                             tGCCTTGCAGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTc  >  1:263266/1‑139 (MQ=255)

CACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTC  >  NC_000913/2871131‑2871394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A