Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,757,183	A→G	C215C (TGT→TGC)	yiaY ←	L‑threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,757,183	0	A	G	100.0%	379.7 / NA	105	C215C (TGT→TGC)	yiaY	L‑threonine dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (49/56); total (49/56)

Only 100 of 106 total aligned reads displayed.

ACCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAAGCAG  >  NC_000913/3757048‑3757317

aCCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCaa                                                                                                                                     >  1:829428/1‑139 (MQ=255)

aCCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGGTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCaa                                                                                                                                     >  1:1007221/1‑139 (MQ=255)

    agagAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGt                                                                                                                                 >  2:633770/1‑139 (MQ=255)

    agagAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGt                                                                                                                                 <  1:1097214/139‑1 (MQ=255)

    agagAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGt                                                                                                                                 >  2:521468/1‑139 (MQ=255)

    agagAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGt                                                                                                                                 >  2:632161/1‑139 (MQ=255)

     gagaAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTc                                                                                                                                <  1:325241/139‑1 (MQ=255)

      agaAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCa                                                                                                                               >  2:922695/1‑139 (MQ=255)

           cATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGAt                                                                                                                          >  2:829828/1‑139 (MQ=255)

           cATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGAt                                                                                                                          >  1:1070440/1‑139 (MQ=255)

                    aCGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggc                                                                                                                 <  2:239547/139‑1 (MQ=255)

                    aCGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggc                                                                                                                 <  2:37187/139‑1 (MQ=255)

                    aCGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggc                                                                                                                 <  1:692609/139‑1 (MQ=255)

                      gCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcgg                                                                                                               <  1:297360/139‑1 (MQ=255)

                       ccATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcggc                                                                                                              <  1:829828/139‑1 (MQ=255)

                         aTACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCaa                                                                                                            <  1:521468/139‑1 (MQ=255)

                          tACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGcgca                                                                                                                                      <  2:312384/112‑1 (MQ=255)

                          tACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGAt                                                                                                                          >  1:229577/1‑124 (MQ=255)

                          tACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcggc                                                                                                              <  2:1174082/136‑1 (MQ=255)

                          tACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcggc                                                                                                              >  1:1174082/1‑136 (MQ=255)

                           aCCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATa                                                                                                          <  1:55735/139‑1 (MQ=255)

                                cGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAAc                                                                                                     >  1:1170671/1‑139 (MQ=255)

                                cGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAAc                                                                                                     >  1:323886/1‑139 (MQ=255)

                                 gAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                    <  1:633770/139‑1 (MQ=255)

                                 gAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                    <  1:1126714/139‑1 (MQ=255)

                                  aGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAg                                                                                                                                    <  2:1178420/106‑1 (MQ=255)

                                  aGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAg                                                                                                                                    >  1:1178420/1‑106 (MQ=255)

                                  aGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCa                                                                                                             >  2:880282/1‑129 (MQ=255)

                                  aGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCa                                                                                                             <  1:880282/129‑1 (MQ=255)

                                   ggAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACata                                                                                                  <  1:632161/139‑1 (MQ=255)

                                   ggAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACata                                                                                                  <  1:304010/139‑1 (MQ=255)

                                       cTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCt                                                                                              <  1:296332/139‑1 (MQ=255)

                                          ggCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCg                                                                                           >  2:521874/1‑139 (MQ=255)

                                           gCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGa                                                                                          <  2:677570/139‑1 (MQ=255)

                                               aaGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACata                                                                                                  >  1:469476/1‑127 (MQ=255)

                                               aaGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACata                                                                                                  <  2:469476/127‑1 (MQ=255)

                                               aaGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgc                                                                                      <  1:348238/139‑1 (MQ=255)

                                                aGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgcg                                                                                     <  1:91826/139‑1 (MQ=255)

                                                aGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgcg                                                                                     <  2:570872/139‑1 (MQ=255)

                                                      tGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGtt                                                                               >  1:102139/1‑139 (MQ=255)

                                                          tCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAgg                                                                           <  2:1070440/139‑1 (MQ=255)

                                                          tCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAgg                                                                           <  2:532374/139‑1 (MQ=255)

                                                            aCGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                    >  2:803647/1‑112 (MQ=255)

                                                            aCGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                    <  1:803647/112‑1 (MQ=255)

                                                            aCGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGtt                                                                               >  2:325908/1‑133 (MQ=255)

                                                            aCGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGtt                                                                               <  1:325908/133‑1 (MQ=255)

                                                                tcTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCg                                                                                                                                  >  1:1195680/2‑78 (MQ=255)

                                                                gcTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCg                                                                                                                                  <  2:1195680/78‑1 (MQ=255)

                                                                  tttCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcggc                                                                                                              <  1:620209/96‑1 (MQ=255)

                                                                         ttACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTg                                                            <  2:674163/139‑1 (MQ=255)

                                                                            cTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTgcgg                                                         >  1:343371/1‑139 (MQ=255)

                                                                               ccATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCg                                                                                           >  1:371219/1‑102 (MQ=255)

                                                                               ccATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCg                                                                                           <  2:371219/102‑1 (MQ=255)

                                                                               ccATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGcggcgg                                                      >  2:357241/1‑139 (MQ=255)

                                                                                  tCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                    >  2:174716/1‑90 (MQ=255)

                                                                                  tCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                    <  1:174716/90‑1 (MQ=255)

                                                                                    ttCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCaa                                                                                                            >  1:924493/1‑80 (MQ=255)

                                                                                    ttCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCaa                                                                                                            <  2:924493/80‑1 (MQ=255)

                                                                                    ttCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTgcgg                                                         <  1:111347/131‑1 (MQ=255)

                                                                                    ttCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTgcgg                                                         >  2:111347/1‑131 (MQ=255)

                                                                                       aaCGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGa                                                                                          >  2:89047/1‑95 (MQ=255)

                                                                                            cTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcg                                                                                                                <  2:776813/68‑1 (MQ=255)

                                                                                            cTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcg                                                                                                                >  1:776813/1‑68 (MQ=255)

                                                                                            cTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACata                                                                                                  <  2:865319/82‑1 (MQ=255)

                                                                                            cTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACata                                                                                                  >  1:865319/1‑82 (MQ=255)

                                                                                            cTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCgg                                         >  1:1131540/1‑139 (MQ=255)

                                                                                            cTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCgg                                         >  1:1081638/1‑139 (MQ=255)

                                                                                              aaCGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCa                                       <  2:1131540/139‑1 (MQ=255)

                                                                                              aaCGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCa                                       >  2:346974/1‑139 (MQ=255)

                                                                                              aaCGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCa                                       >  1:1163412/1‑139 (MQ=255)

                                                                                              aaCGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCa                                       >  2:1031885/1‑139 (MQ=255)

                                                                                              aaCGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCa                                       <  1:995471/139‑1 (MQ=255)

                                                                                                     ggTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAat                                >  2:96329/1‑139 (MQ=255)

                                                                                                     ggTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAat                                >  2:816256/1‑139 (MQ=255)

                                                                                                      gTTTTCGGCAATCATGGTTACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcg                                                                                                                <  1:310121/58‑1 (MQ=255)

                                                                                                      gTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcg                                                                                                                >  2:310121/1‑58 (MQ=255)

                                                                                                       ttttCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAatca                              <  1:1171350/139‑1 (MQ=255)

                                                                                                        tttCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAatcat                             <  1:576981/139‑1 (MQ=255)

                                                                                                           cGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCag                          >  1:903509/1‑139 (MQ=255)

                                                                                                             gCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgc                                                                                      <  2:1218534/77‑1 (MQ=255)

                                                                                                             gCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgc                                                                                      >  1:1218534/1‑77 (MQ=255)

                                                                                                               aaTCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCagagag                      <  2:1081638/139‑1 (MQ=255)

                                                                                                                 tCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAgagga                    <  2:102139/139‑1 (MQ=255)

                                                                                                                 tCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAgagga                    <  2:223172/139‑1 (MQ=255)

                                                                                                                      gTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCAt               <  1:357241/139‑1 (MQ=255)

                                                                                                                         aCGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcg                                                                                                                >  1:273439/1‑39 (MQ=38)

                                                                                                                         aCGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcg                                                                                                                <  2:273439/39‑1 (MQ=255)

                                                                                                                          cGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCt                                                                                              >  1:278881/1‑56 (MQ=255)

                                                                                                                          cGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCt                                                                                              <  2:278881/56‑1 (MQ=255)

                                                                                                                          cGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACtt                                            >  2:303613/1‑106 (MQ=255)

                                                                                                                          cGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACtt                                            <  1:303613/106‑1 (MQ=255)

                                                                                                                           ggCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATa                                                                                                          >  2:680880/1‑43 (MQ=255)

                                                                                                                           ggCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATa                                                                                                          <  1:680880/43‑1 (MQ=255)

                                                                                                                           ggCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgcgtgcg                                                                                 <  1:997455/68‑1 (MQ=255)

                                                                                                                           ggCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACa          >  1:359647/1‑139 (MQ=255)

                                                                                                                                tCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCTATCATCAGAGAGGAGTCATTGACAGAAag     >  1:215258/1‑139 (MQ=255)

                                                                                                                                   gTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTc                                                    <  1:592606/89‑1 (MQ=255)

                                                                                                                                   gTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTc                                                    >  2:592606/1‑89 (MQ=255)

                                                                                                                                   gTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAagcag  <  1:346974/139‑1 (MQ=255)

                                                                                                                                   gTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAagcag  <  2:829428/139‑1 (MQ=255)

ACCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAAGCAG  >  NC_000913/3757048‑3757317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A