Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I2 R1
|
780 |
68.3 |
3343050 |
93.5% |
3125751 |
102.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,926,801 |
A→G |
V68A (GTA→GCA) |
asnC ← |
transcriptional activator of asnA, autorepressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,926,801 | 0 | A | G | 98.5%
| 237.9
/ ‑5.0
| 68 | V68A (GTA→GCA) | asnC | transcriptional activator of asnA, autorepressor |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (39/28); total (40/28) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 |
GCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGC > NC_000913/3926686‑3926934
|
gCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCtt > 1:1440717/1‑135 (MQ=255)
gCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTGTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCtt < 2:1440717/135‑1 (MQ=255)
cTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGc > 2:381355/1‑139 (MQ=255)
gCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCa > 2:790886/1‑139 (MQ=255)
ggTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATa > 2:365713/1‑139 (MQ=255)
ttGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCa < 2:498309/107‑1 (MQ=255)
ttGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCa > 1:498309/1‑107 (MQ=255)
ttGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcg < 1:365713/139‑1 (MQ=255)
tAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGccc > 1:1450002/1‑139 (MQ=255)
tAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGccc < 1:790886/139‑1 (MQ=255)
ggCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTa > 1:1341388/1‑139 (MQ=255)
ttCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAgctgct > 2:1591493/1‑107 (MQ=255)
ttCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAgctgct < 1:1591493/107‑1 (MQ=255)
ttCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcgcg < 1:447097/128‑1 (MQ=255)
ttCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcgcg > 2:447097/1‑128 (MQ=255)
cAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTg > 2:972276/1‑113 (MQ=255)
cAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGCTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGAGCTg < 1:972276/113‑1 (MQ=255)
ttCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGcc > 2:228891/1‑139 (MQ=255)
atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGccc < 2:895532/119‑1 (MQ=255)
atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGccc > 1:895532/1‑119 (MQ=255)
cTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCAttt > 1:1317000/1‑139 (MQ=255)
tttCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCAtttt < 2:1317000/139‑1 (MQ=255)
ccAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAAc > 1:557099/1‑139 (MQ=255)
cgcgGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCt > 2:1579443/1‑78 (MQ=255)
cgcgGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCt < 1:1579443/78‑1 (MQ=255)
aaGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGt > 1:1420164/1‑139 (MQ=255)
gcgcTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGc > 2:429557/1‑139 (MQ=255)
cgcTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGcc > 1:462191/1‑139 (MQ=255)
tctTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCaaa > 2:358/1‑139 (MQ=255)
ctTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACt < 1:955728/106‑1 (MQ=255)
ctTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACt > 2:955728/1‑106 (MQ=255)
tttAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAAt < 2:1434661/116‑1 (MQ=255)
tttAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAAt > 1:1434661/1‑116 (MQ=255)
aaTGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCtt < 1:443480/39‑1 (MQ=255)
aaTGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCtt > 2:443480/1‑39 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCggg < 1:226724/41‑1 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCggg > 2:226724/1‑41 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGGCATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg > 1:1182353/1‑139 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc > 2:728308/1‑58 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc < 1:728308/58‑1 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGcccc < 2:313203/61‑1 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGcccc > 1:313203/1‑61 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTaa > 1:1602378/1‑66 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTaa < 2:1602378/66‑1 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGc > 1:1654892/1‑80 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGc < 2:1654892/80‑1 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACt > 2:178519/1‑95 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACt < 1:178519/95‑1 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGt > 2:491360/1‑131 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGt < 1:491360/131‑1 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg > 1:122894/1‑139 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg > 2:102008/1‑139 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg > 1:1581511/1‑139 (MQ=255)
tGCCGATAAAGCAGCCTGCGTCATAAACGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTATTTCGCCAg > 1:131514/1‑139 (MQ=255)
gCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGt < 2:1420164/139‑1 (MQ=255)
gCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGt < 2:1182353/139‑1 (MQ=255)
ccGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGtt < 2:462191/139‑1 (MQ=255)
ccGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGtt > 2:1015766/1‑139 (MQ=255)
ccGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGtt > 2:620183/1‑139 (MQ=255)
cGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTc < 2:1581511/139‑1 (MQ=255)
aTAAAGCAGCCTGCGTCATAGCCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACg > 1:981349/1‑96 (MQ=255)
aTAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCt > 1:1659896/1‑76 (MQ=255)
aTAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCt < 2:1659896/76‑1 (MQ=255)
aTAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACg < 2:981349/96‑1 (MQ=255)
aTAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCgg > 2:690062/1‑139 (MQ=255)
agcCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGc < 1:690062/139‑1 (MQ=255)
gcCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc < 1:467585/45‑1 (MQ=255)
gcCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc > 2:467585/1‑45 (MQ=255)
|
GCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGC > NC_000913/3926686‑3926934
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
