Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
771 |
44.8 |
2153074 |
94.1% |
2026042 |
109.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,689,625 |
C→T |
A213T (GCC→ACC) |
bcsZ ← |
endo‑1,4‑D‑glucanase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,689,625 | 0 | C | T | 88.0%
| 54.7
/ 1.4
| 25 | A213T (GCC→ACC) | bcsZ | endo‑1,4‑D‑glucanase |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (12/10); total (13/12) |
| Fisher's exact test for biased strand distribution p-value = 5.93e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 |
TCATGCCTACCCACATGTAAACGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCC > NC_000913/3689502‑3689735
|
tcatGCCTACCCACATGTAAACGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCaa > 1:479781/1‑136 (MQ=255)
tcatGCCTACCCACATGTAAACGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCaa < 2:479781/136‑1 (MQ=255)
aTGTAAACGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGttt > 1:530144/1‑139 (MQ=255)
gTAAACGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTcg > 1:917810/1‑139 (MQ=255)
aaaCGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCGGCCAGCCTTTGTCTTTCTCATAGCGCAACTAGTCTGGCGAAAATCATTTCGGGGTGGTTTCCAGCAATAATTGTTGATTGGTTTcgcg < 2:1042847/139‑1 (MQ=255)
aCGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGGCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCa < 2:548247/139‑1 (MQ=255)
gcgGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGc > 2:575439/1‑139 (MQ=255)
ctgctgATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGgcgcgc > 2:590651/1‑139 (MQ=255)
aTCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGc < 1:590651/139‑1 (MQ=255)
cAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGAt < 1:447050/101‑1 (MQ=255)
cAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGAt > 2:447050/1‑101 (MQ=255)
tGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTg > 1:378447/1‑139 (MQ=255)
gTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGa < 2:462223/139‑1 (MQ=255)
ttttttCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGaa > 2:710031/1‑139 (MQ=255)
cAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTcggcg < 2:283980/139‑1 (MQ=255)
ccTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCa > 1:741790/1‑139 (MQ=255)
aGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCgg < 2:480928/95‑1 (MQ=255)
aGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCgg > 1:480928/1‑95 (MQ=255)
aCCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGcc < 2:378447/139‑1 (MQ=255)
aCCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGcc > 2:554348/1‑139 (MQ=255)
aCCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGcc < 2:917810/139‑1 (MQ=255)
gCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGAt < 1:900449/42‑1 (MQ=255)
gCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGAt > 2:900449/1‑42 (MQ=255)
tttCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCa > 1:1035071/1‑44 (MQ=255)
tttCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCa < 2:1035071/44‑1 (MQ=255)
|
TCATGCCTACCCACATGTAAACGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCC > NC_000913/3689502‑3689735
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
