Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,689,625 |
C→T |
A213T (GCC→ACC) |
bcsZ ← |
endo‑1,4‑D‑glucanase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,689,625 | 0 | C | T | 100.0%
| 92.4
/ NA
| 30 | A213T (GCC→ACC) | bcsZ | endo‑1,4‑D‑glucanase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/14); total (16/14) |
GTCAGGCATCATGCCTACCCACATGTAAACGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCA > NC_000913/3689494‑3689736
|
gTCAGGCATCATGCCTACCCACATGTAAACGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTcc < 1:736250/139‑1 (MQ=255)
tGCCTACCCACATGTAAACGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACg > 1:309740/1‑139 (MQ=255)
acaTGTAAACGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGt > 2:1049347/1‑139 (MQ=255)
aCGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGAt < 1:279992/127‑1 (MQ=255)
aCGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGAt > 2:279992/1‑127 (MQ=255)
tAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGgcg > 1:147811/1‑139 (MQ=255)
gctgATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCa > 1:194167/1‑139 (MQ=255)
gctgATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCa > 2:581246/1‑139 (MQ=255)
tgATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCaaa < 2:194167/139‑1 (MQ=255)
aTCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGc < 1:1049347/139‑1 (MQ=255)
tttCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGAt < 2:605024/93‑1 (MQ=255)
tttCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGAt > 1:605024/1‑93 (MQ=255)
gCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGt < 2:147811/139‑1 (MQ=255)
ccAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACg > 2:928782/1‑72 (MQ=255)
ccAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACg < 1:928782/72‑1 (MQ=255)
tttGTCTTTCTCATAGCGTACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAgg < 1:1135621/139‑1 (MQ=255)
ctcATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTc < 1:736827/45‑1 (MQ=255)
ctcATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTc > 2:736827/1‑45 (MQ=255)
aGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCaa > 2:430293/1‑90 (MQ=255)
aGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCaa < 1:430293/90‑1 (MQ=255)
gcgcACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCgg < 1:889016/94‑1 (MQ=255)
gcgcACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCgg > 2:889016/1‑94 (MQ=255)
cgcACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAAc > 2:1139440/1‑139 (MQ=255)
cACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATa > 1:498248/1‑44 (MQ=255)
cACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATa < 2:498248/44‑1 (MQ=255)
cACCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGc > 2:910026/1‑139 (MQ=255)
aCCCAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGcc < 1:581246/139‑1 (MQ=255)
cccAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCa > 2:211723/1‑139 (MQ=255)
ccAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTcgcg > 2:1313771/1‑60 (MQ=255)
ccAGTCTGGCGAAAAACCTTTCGGGGTGGTTTCCAGCAATAAACGTTGATTGGTTTcgcg < 1:1313771/60‑1 (MQ=255)
|
GTCAGGCATCATGCCTACCCACATGTAAACGCGGATAGCGTCGTAGCTGCTGATCAATGTTTTTTCGGCTTTTAGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCA > NC_000913/3689494‑3689736
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
