Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I2 R1 772 77.0 3769570 91.6% 3452926 105.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,015,738 G→A *193* (TAG→TAA)  mocA → CTP:molybdopterin cytidylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,015,7380GA100.0% 224.9 / NA 68*193* (TAG→TAA) mocACTP:molybdopterin cytidylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/31);  total (37/31)

CCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAT  >  NC_000913/3015611‑3015872
                                                                                                                               |                                                                                                                                      
ccAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGtt                                                                                                                             >  2:279595/1‑139 (MQ=255)
ccAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGtt                                                                                                                             >  2:941769/1‑139 (MQ=255)
   atatGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTaaa                                                                                                                          <  1:279595/139‑1 (MQ=255)
      tGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGa                                                                                                                       >  1:698218/1‑139 (MQ=255)
                            agaTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCt                                                                                                 >  2:1236514/1‑139 (MQ=255)
                            agaTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCt                                                                                                 >  2:78722/1‑139 (MQ=255)
                              aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCaa                                                                                                             <  1:895625/125‑1 (MQ=255)
                              aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCaa                                                                                                             >  2:895625/1‑125 (MQ=255)
                              aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTc                                                                                               >  2:592604/1‑139 (MQ=255)
                                 attattCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCgg                                                                                            <  1:941769/139‑1 (MQ=255)
                                   tattCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAATGAAAGGTATACTGAAATTTAAAAATCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGcg                                                                                          <  1:1236514/139‑2 (MQ=255)
                                   tattCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                          <  2:698218/139‑1 (MQ=255)
                                   tattCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                          <  1:78722/139‑1 (MQ=255)
                                     ttCCGTCGAAATAGAAAATACAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                                        >  2:1458909/1‑139 (MQ=255)
                                        cGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCAt                                                                                     <  2:1863936/139‑1 (MQ=255)
                                          tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAAt                                                                                                    <  2:532380/122‑1 (MQ=255)
                                          tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAAt                                                                                                    >  1:532380/1‑122 (MQ=255)
                                          tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                          <  1:1443510/132‑1 (MQ=255)
                                          tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                          >  2:1443510/1‑132 (MQ=255)
                                          tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                                        <  2:1684994/134‑1 (MQ=255)
                                          tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                                        >  1:1684994/1‑134 (MQ=255)
                                              aaTAGAAAATACAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCAt                                                                               <  1:1458909/139‑1 (MQ=255)
                                               aTAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATa                                                                              <  1:592604/139‑1 (MQ=255)
                                                            aaaTAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGa                                                                    >  1:583293/1‑136 (MQ=255)
                                                            aaaTAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGa                                                                    <  2:583293/136‑1 (MQ=255)
                                                                      aGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCAt                                                                                     <  1:719510/109‑1 (MQ=255)
                                                                      aGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCAt                                                                                     >  2:719510/1‑109 (MQ=255)
                                                                      aGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTg                                                       >  1:1683199/1‑139 (MQ=255)
                                                                      aGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTg                                                       >  2:792955/1‑139 (MQ=255)
                                                                          atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                                        >  2:964967/1‑102 (MQ=255)
                                                                          atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                                        <  1:964967/102‑1 (MQ=255)
                                                                                 cTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagagaa                                            <  2:1683199/139‑1 (MQ=255)
                                                                                  tCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCa                                                                <  1:234375/118‑1 (MQ=255)
                                                                                  tCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCa                                                                >  2:234375/1‑118 (MQ=255)
                                                                                   ccGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTaaaaaa                                                                                                                                    <  2:381789/49‑1 (MQ=255)
                                                                                   ccGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTaaaaaa                                                                                                                                    >  1:381789/1‑49 (MQ=255)
                                                                                    cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                          <  2:597634/90‑1 (MQ=255)
                                                                                    cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                          >  1:597634/1‑90 (MQ=255)
                                                                                    cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCAt                                                                                     >  1:1531144/1‑95 (MQ=255)
                                                                                    cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCAt                                                                                     <  2:1531144/95‑1 (MQ=255)
                                                                                    cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCAt                                                                               <  2:793622/101‑1 (MQ=255)
                                                                                    cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCAt                                                                               >  1:793622/1‑101 (MQ=255)
                                                                                    cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaa                                                   >  2:842468/1‑129 (MQ=255)
                                                                                    cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaa                                                   <  1:842468/129‑1 (MQ=255)
                                                                                     ggACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCg                                                                                                                  >  2:186356/1‑65 (MQ=255)
                                                                                     ggACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCg                                                                                                                  <  1:186356/65‑1 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGAt                                                                                                                      >  1:511504/1‑60 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGAt                                                                                                                      <  2:511504/60‑1 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAAt                                                                            <  1:69935/102‑1 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAAt                                                                            >  2:69935/1‑102 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaa                                                   >  2:1826745/1‑127 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaa                                                   <  1:1826745/127‑1 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAAtt                                       >  2:924808/1‑139 (MQ=255)
                                                                                            tttATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTGCAAATATTTAATCCTTCCGACACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATaaa                                 >  2:1176788/1‑139 (MQ=255)
                                                                                                      cGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGc                                                                                           >  1:318440/1‑71 (MQ=255)
                                                                                                      cGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGc                                                                                           <  2:318440/71‑1 (MQ=255)
                                                                                                      cGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATa                                                                        >  1:1021016/1‑90 (MQ=255)
                                                                                                      cGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATa                                                                        <  2:1021016/90‑1 (MQ=255)
                                                                                                        aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                          >  2:1193556/1‑70 (MQ=255)
                                                                                                        aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                          <  1:1193556/70‑1 (MQ=255)
                                                                                                        aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCaat                     >  2:1157302/1‑139 (MQ=255)
                                                                                                        aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCaat                     >  1:980917/1‑139 (MQ=255)
                                                                                                        aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCACAATGATAAGGAAt                                                                  >  2:1840555/1‑94 (MQ=255)
                                                                                                        aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCACAATGATAAGGAAt                                                                  <  1:1840555/94‑1 (MQ=255)
                                                                                                                       gAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAg      >  2:1554614/1‑139 (MQ=255)
                                                                                                                          aTTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCAt                                                                                     >  2:341948/1‑57 (MQ=255)
                                                                                                                          aTTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCAt                                                                                     <  1:341948/57‑1 (MQ=255)
                                                                                                                           tttAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagagaa                                            <  2:294712/97‑1 (MQ=255)
                                                                                                                           tttAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagagaa                                            >  1:294712/1‑97 (MQ=255)
                                                                                                                           tttAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAt  >  2:1433915/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                      
CCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAT  >  NC_000913/3015611‑3015872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A