Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I2 R1 764 42.0 2018750 95.5% 1927906 109.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,015,738 G→A *193* (TAG→TAA)  mocA → CTP:molybdopterin cytidylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,015,7380GA100.0% 172.1 / NA 53*193* (TAG→TAA) mocACTP:molybdopterin cytidylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/25);  total (28/25)

CCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAG  >  NC_000913/3015611‑3015868
                                                                                                                               |                                                                                                                                  
ccAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGtt                                                                                                                         >  1:582420/1‑139 (MQ=255)
    tatGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAAc                                                                                                                     >  1:621679/1‑139 (MQ=255)
          tCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGc                                                                                                               >  1:189657/1‑139 (MQ=255)
              gCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAg                                                                                                                           <  2:322490/123‑1 (MQ=255)
              gCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAg                                                                                                                           >  1:322490/1‑123 (MQ=255)
                       aTGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTa                                                                                                  >  2:848917/1‑139 (MQ=255)
                              aTCTTTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTc                                                                                           >  1:387488/1‑139 (MQ=255)
                              aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAAc                                                                                                                               >  2:468025/1‑103 (MQ=255)
                              aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAAc                                                                                                                               <  1:468025/103‑1 (MQ=255)
                              aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTaa                                                                                                 <  2:333446/133‑1 (MQ=255)
                              aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTaa                                                                                                 >  1:333446/1‑133 (MQ=255)
                              aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTc                                                                                           >  2:802677/1‑139 (MQ=255)
                              aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTc                                                                                           >  1:647529/1‑139 (MQ=255)
                                tattattTCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGATTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCg                                                                                         <  1:612873/138‑1 (MQ=255)
                                 attattCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCgg                                                                                        <  2:189657/139‑1 (MQ=255)
                                   tattCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                      <  2:90992/139‑1 (MQ=255)
                                     ttCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                                    <  2:621679/139‑1 (MQ=255)
                                          tCGAAATAGAAAATGCATAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTa                                                                               >  2:631759/1‑139 (MQ=255)
                                          tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTa                                                                               >  2:975851/1‑139 (MQ=255)
                                          tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTa                                                                               >  2:110854/1‑139 (MQ=255)
                                          tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTAATTa                                                                               >  2:344239/1‑139 (MQ=255)
                                                tAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATaa                                                                         <  1:975851/139‑1 (MQ=255)
                                                     aaTGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATa                                                                    <  1:662907/139‑1 (MQ=255)
                                                             aataatTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCa                                                            >  1:532778/1‑139 (MQ=255)
                                                                                     ggACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCgg                                                                                        >  2:606404/1‑87 (MQ=255)
                                                                                     ggACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCgg                                                                                        <  1:606404/87‑1 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTCTACTGAAATTTAAAACACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATa                                                                          >  2:928084/1‑100 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCa                                                                                                          <  2:241605/68‑1 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCa                                                                                                          >  1:241605/1‑68 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCgg                                                                                        <  1:346773/86‑1 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCgg                                                                                        >  2:346773/1‑86 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCa                                                                                  <  1:385917/92‑1 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCa                                                                                  >  2:385917/1‑92 (MQ=255)
                                                                                      gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATa                                                                          <  1:928084/100‑1 (MQ=255)
                                                                                            tttATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATaaa                             >  2:761620/1‑139 (MQ=255)
                                                                                                  aCAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCt                       <  2:532778/139‑1 (MQ=255)
                                                                                                     gCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCa                                                                                                          >  2:172676/1‑53 (MQ=255)
                                                                                                     gCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCa                                                                                                          <  1:172676/53‑1 (MQ=255)
                                                                                                     gCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                                    >  1:985938/1‑75 (MQ=255)
                                                                                                     gCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                                    <  2:985938/75‑1 (MQ=255)
                                                                                                     gCGAAGGAAAGGAATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGc                    <  1:344239/139‑1 (MQ=255)
                                                                                                        aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCg                                                                                                              <  2:279118/46‑1 (MQ=255)
                                                                                                        aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCg                                                                                                              >  1:279118/1‑46 (MQ=255)
                                                                                                        aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCgg                                                                                        <  2:714986/68‑1 (MQ=255)
                                                                                                        aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTgga                    >  1:170654/1‑135 (MQ=255)
                                                                                                        aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGc                    <  2:170654/136‑1 (MQ=255)
                                                                                                        aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCaat                 >  2:619787/1‑139 (MQ=255)
                                                                                                        aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGAGTTCAAATATTTAATCCTTCCgg                                                                                        >  1:714986/1‑68 (MQ=255)
                                                                                                            aaaGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCaataata             <  1:802677/139‑1 (MQ=255)
                                                                                                                    aCTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCa                                                                                  <  2:10752/62‑1 (MQ=255)
                                                                                                                    aCTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCa                                                                                  >  1:10752/1‑62 (MQ=255)
                                                                                                                      tGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGa   <  1:918999/139‑1 (MQ=255)
                                                                                                                       gAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAg  <  2:582420/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                                  
CCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAG  >  NC_000913/3015611‑3015868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A