Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,440,950	G→A	T27I (ACC→ATC)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,440,950	0	G	A	98.9%	284.5 / NA	89	T27I (ACC→ATC)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (45/43); total (45/44)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGG  >  NC_000913/3440824‑3441082

cTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGgcgt                                                                                                                          >  1:1738250/1‑139 (MQ=255)

   tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                       >  2:557652/1‑139 (MQ=255)

        aTGTAGTACACCAGCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGaa                                                                                                                  >  1:1260390/1‑139 (MQ=255)

             gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                             <  1:564623/139‑1 (MQ=255)

             gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                             <  1:557652/139‑1 (MQ=255)

             gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                             >  2:1328502/1‑139 (MQ=255)

              taCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACt                                                                                                            <  2:435217/139‑1 (MQ=255)

               acacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACtt                                                                                                           >  2:527844/1‑139 (MQ=255)

                cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTg                                                                                                          >  1:1072144/1‑139 (MQ=255)

                cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTg                                                                                                          >  1:591694/1‑139 (MQ=255)

                cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTg                                                                                                          >  1:1280833/1‑139 (MQ=255)

                  ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                        >  2:29120/1‑139 (MQ=255)

                  ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                        <  2:1795337/139‑1 (MQ=255)

                  ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                        <  1:542002/139‑1 (MQ=255)

                    aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCg                                                                                                      >  2:1466430/1‑139 (MQ=255)

                     tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGa                                                                                                     >  2:612872/1‑139 (MQ=255)

                        aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                                  >  1:1264617/1‑139 (MQ=255)

                               ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAg                                                                                           >  2:590319/1‑139 (MQ=255)

                               ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAg                                                                                           >  1:148765/1‑139 (MQ=255)

                                  cGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                        <  1:29120/139‑1 (MQ=255)

                                  cGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                        <  1:527844/139‑1 (MQ=255)

                                   ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGc                                                                                                         >  2:192235/1‑121 (MQ=255)

                                   ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGc                                                                                                         <  1:192235/121‑1 (MQ=255)

                                      cACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGt                                                                                    <  1:1466430/139‑1 (MQ=255)

                                         cgcgCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCAcc                                                                                                                                 <  2:858093/91‑1 (MQ=255)

                                         cgcgCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCAcc                                                                                                                                 >  1:858093/1‑91 (MQ=255)

                                         cgcgCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACTTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGtttt                                                                                 >  2:718026/1‑139 (MQ=255)

                                           cgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                       >  2:1125238/1‑99 (MQ=255)

                                           cgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                       <  1:1125238/99‑1 (MQ=255)

                                            gcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGa                                                                              <  2:148765/139‑1 (MQ=255)

                                              aaCCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGaaa                                                                            >  2:942258/1‑139 (MQ=255)

                                                cccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAAct                                                                          <  1:1319192/139‑1 (MQ=255)

                                                 ccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACTTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                                         <  1:718026/139‑1 (MQ=255)

                                                 ccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                                         <  2:1072144/139‑1 (MQ=255)

                                                   ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTg                                                                       >  1:1526708/1‑139 (MQ=255)

                                                   ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTg                                                                       <  1:590319/139‑1 (MQ=255)

                                                   ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTg                                                                       >  1:1787103/1‑139 (MQ=255)

                                                          aggagaGCAGAATACGGCGCAGTGCGTTAGCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTCGCTCGATATCAAGCAGGCGCGGTTTTAGAAACTCTGTCACAGa                                                                <  1:639319/137‑1 (MQ=255)

                                                          aTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGcgcg                                                                                      >  2:1604501/1‑117 (MQ=255)

                                                          aTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGcgcg                                                                                      <  1:1604501/117‑1 (MQ=255)

                                                          aTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGa                                                                >  2:1344552/1‑139 (MQ=255)

                                                              gagCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAccc                                                            <  2:1280833/139‑1 (MQ=255)

                                                                      tACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtgt                                                    >  2:645819/1‑139 (MQ=255)

                                                                      tACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtgt                                                    <  1:645819/139‑1 (MQ=255)

                                                                         ggCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                                 <  2:1738250/139‑1 (MQ=255)

                                                                           cgcAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                        >  2:1523608/1‑82 (MQ=255)

                                                                           cgcAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                        <  1:1523608/82‑1 (MQ=255)

                                                                           cgcAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                               <  2:505901/139‑1 (MQ=255)

                                                                           cgcAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                               <  2:407292/139‑1 (MQ=255)

                                                                                tGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGa                                                                <  2:1704246/117‑1 (MQ=255)

                                                                                tGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGa                                                                >  1:1704246/1‑117 (MQ=255)

                                                                                tGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                          <  2:1264617/139‑1 (MQ=255)

                                                                                      aCCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTAc                                    <  2:1787103/139‑1 (MQ=255)

                                                                                            agTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgct                                                                                        <  1:1172460/81‑2 (MQ=255)

                                                                                            agTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                        >  2:1172460/1‑81 (MQ=255)

                                                                                            agTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCt                              >  1:550266/1‑139 (MQ=255)

                                                                                                 ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                             >  1:1000756/1‑55 (MQ=255)

                                                                                                 ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                             <  2:1000756/55‑1 (MQ=255)

                                                                                                 ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                                         >  2:783571/1‑91 (MQ=255)

                                                                                                 ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                                         <  1:783571/91‑1 (MQ=255)

                                                                                                 ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                          >  1:1393122/1‑122 (MQ=255)

                                                                                                 ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                          <  1:1342487/122‑1 (MQ=255)

                                                                                                 ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                          >  2:1342487/1‑122 (MQ=255)

                                                                                                 ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                          <  2:1393122/122‑1 (MQ=255)

                                                                                                 ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACt                                   >  2:45049/1‑129 (MQ=255)

                                                                                                 ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACt                                   <  1:45049/129‑1 (MQ=255)

                                                                                                 ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt                         >  2:1726656/1‑139 (MQ=255)

                                                                                                 ggCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt                         >  1:1084928/1‑139 (MQ=255)

                                                                                                  gCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                        >  2:1189320/1‑59 (MQ=255)

                                                                                                  gCCAAAGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                        <  1:1189320/59‑1 (MQ=255)

                                                                                                      aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                  <  2:958230/93‑1 (MQ=255)

                                                                                                      aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                  >  1:958230/1‑93 (MQ=255)

                                                                                                      aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAg                    >  2:951257/1‑139 (MQ=255)

                                                                                                      aaGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCACGATATCAACCAGGCGCGGTTTTAGAAACGCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAg                    >  1:1236611/1‑139 (MQ=255)

                                                                                                       aGCCACGCTCTAAAGGCTCAAGTATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGt                   <  1:1726656/139‑1 (MQ=255)

                                                                                                       aGCCACGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGt                   >  2:126621/1‑139 (MQ=255)

                                                                                                            cGCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGc              >  2:490564/1‑139 (MQ=255)

                                                                                                             gCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCt             <  2:591694/139‑1 (MQ=255)

                                                                                                                   aaGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTcgacga       <  1:951257/139‑1 (MQ=255)

                                                                                                                     ggCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTGCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATc     <  1:1328502/139‑1 (MQ=255)

                                                                                                                     ggCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATc     <  2:1260390/139‑1 (MQ=255)

                                                                                                                        tCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAgg  >  2:596249/1‑139 (MQ=255)

                                                                                                                         cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGa                                                                              <  1:1317241/62‑1 (MQ=255)

                                                                                                                         cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGa                                                                              >  2:1317241/1‑62 (MQ=255)

                                                                                                                          aaGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                        <  1:1106195/35‑1 (MQ=255)

                                                                                                                          aaGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                        >  2:1106195/1‑35 (MQ=255)

                                                                                                                           aGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                               <  2:979328/91‑1 (MQ=255)

                                                                                                                           aGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGAt      <  2:754425/132‑1 (MQ=255)

                                                                                                                           aGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGAt      >  1:754425/1‑132 (MQ=255)

CTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGG  >  NC_000913/3440824‑3441082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A