Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,453,610	G→A	A106A (GCC→GCT)	gspB ←	part of gsp divergon involved in type II protein secretion

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,453,610	0	G	A	100.0%	211.4 / NA	65	A106A (GCC→GCT)	gspB	part of gsp divergon involved in type II protein secretion
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (35/30); total (35/30)

GCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACT  >  NC_000913/3453476‑3453739

gCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCAt                                                                                                                               >  1:75570/1‑139 (MQ=255)

          agtaaagtaaACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACcgc                                                                                                                     >  2:717264/1‑139 (MQ=255)

          agtaaagtaaACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACcgc                                                                                                                     >  1:1589707/1‑139 (MQ=255)

           gtaaagtaaaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACcgcg                                                                                                                    <  2:907893/139‑1 (MQ=255)

              aagtaaaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACg                                                                                                                 >  2:869335/1‑139 (MQ=255)

                  aaaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTg                                                                                                             >  2:363417/1‑139 (MQ=255)

                   aaCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATCCTCAACCGCGACGCCTgg                                                                                                            >  1:770388/1‑139 (MQ=255)

                    aCAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTggg                                                                                                           <  1:1297056/139‑1 (MQ=255)

                           tCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCtt                                                                                                    >  2:756382/1‑139 (MQ=255)

                                  aTCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCctcctc                                                                                             >  1:860125/1‑139 (MQ=255)

                                  aTCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCctcctc                                                                                             >  1:27861/1‑139 (MQ=255)

                                  aTCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCctcctc                                                                                             >  1:144249/1‑139 (MQ=255)

                                   tctctcAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCg                                                                                            <  2:1435127/139‑1 (MQ=255)

                                   tctctcAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCg                                                                                            <  2:27861/139‑1 (MQ=255)

                                      ctcAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACg                                                                                         <  1:756382/139‑1 (MQ=255)

                                              cgttcAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTcgac                                                                                 >  1:316521/1‑139 (MQ=255)

                                              cgttcAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTcgac                                                                                 >  2:249411/1‑139 (MQ=255)

                                              cgttcAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTcgac                                                                                 >  1:674506/1‑139 (MQ=255)

                                               gttcAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTcgacg                                                                                >  2:846809/1‑139 (MQ=255)

                                                ttcAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTcgacga                                                                               <  1:249411/139‑1 (MQ=255)

                                                  cAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGacgacg                                                                             <  1:1550124/139‑1 (MQ=255)

                                                  cAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGacgacg                                                                             <  2:674506/139‑1 (MQ=255)

                                                     tGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACgggg                                                                          >  1:376492/1‑139 (MQ=255)

                                                     tGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACgggg                                                                          >  2:297969/1‑139 (MQ=255)

                                                                  cGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAAACGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGttttt                                                             >  2:1003761/1‑139 (MQ=255)

                                                                      ctctTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTgaa                                                         >  1:1417511/1‑139 (MQ=255)

                                                                          tAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGaaa                                                     <  1:717264/139‑1 (MQ=255)

                                                                          tAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGaaa                                                     <  2:860125/139‑1 (MQ=255)

                                                                            aaCCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATa                                                   <  1:1015188/139‑1 (MQ=255)

                                                                              ccGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAgt                                                 <  2:1115343/139‑1 (MQ=255)

                                                                               cGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAgtg                                                >  2:111772/1‑139 (MQ=255)

                                                                                 gCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAgtgtt                                              >  1:1225641/1‑139 (MQ=255)

                                                                                 gCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAgtgtt                                              >  1:1380068/1‑139 (MQ=255)

                                                                                        ctTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGa                                       <  1:1003761/139‑1 (MQ=255)

                                                                                             cTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGtt                                  >  2:49253/1‑139 (MQ=255)

                                                                                                 cttcCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTaaa                              >  2:486206/1‑139 (MQ=255)

                                                                                                   tcCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAg                            <  1:601198/139‑1 (MQ=255)

                                                                                                      acaGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCgg                         <  2:1589707/139‑1 (MQ=255)

                                                                                                       caGTATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCggg                        <  2:144249/139‑1 (MQ=255)

                                                                                                          tATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGt                     >  2:427164/1‑139 (MQ=255)

                                                                                                          tATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGt                     >  1:1623375/1‑139 (MQ=255)

                                                                                                          tATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGt                     >  1:834089/1‑139 (MQ=255)

                                                                                                          tATTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTAGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGt                     >  1:1271996/1‑139 (MQ=255)

                                                                                                           aTTTTCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGTCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGtt                    <  1:363417/139‑1 (MQ=255)

                                                                                                            ttttCTTCGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGttt                   >  2:709284/1‑139 (MQ=255)

                                                                                                                cttcGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGc               <  2:275757/139‑1 (MQ=255)

                                                                                                                cttcGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGc               <  2:316521/139‑1 (MQ=255)

                                                                                                                cttcGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGc               <  1:49253/139‑1 (MQ=255)

                                                                                                                  tcGTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCct             <  2:1225641/139‑1 (MQ=255)

                                                                                                                    gTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGacgacg                                                                             <  1:1039333/73‑1 (MQ=255)

                                                                                                                    gTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGacgacg                                                                             >  2:1039333/1‑73 (MQ=255)

                                                                                                                    gTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTgaag                                                        <  1:330242/94‑1 (MQ=255)

                                                                                                                    gTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTgaag                                                        >  2:330242/1‑94 (MQ=255)

                                                                                                                    gTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTga                                                          >  1:588204/1‑92 (MQ=255)

                                                                                                                    gTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTga                                                          <  2:588204/92‑1 (MQ=255)

                                                                                                                    gTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCctct           >  1:529123/1‑139 (MQ=255)

                                                                                                                    gTCTTCAGAAGAAGAGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCctct           >  2:1277053/1‑139 (MQ=255)

                                                                                                                           gaagaagaGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTc                  <  2:391382/125‑1 (MQ=255)

                                                                                                                           gaagaagaGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTc                  >  1:391382/1‑125 (MQ=255)

                                                                                                                             agaagaGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACt  <  1:774634/139‑1 (MQ=255)

                                                                                                                             agaagaGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACt  <  1:869335/139‑1 (MQ=255)

                                                                                                                             agaagaGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACt  <  1:427164/139‑1 (MQ=255)

                                                                                                                             agaagaGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACt  <  2:1623375/139‑1 (MQ=255)

                                                                                                                               aagaGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCg                                                                                            <  1:1178089/47‑1 (MQ=255)

                                                                                                                               aagaGGGAGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCg                                                                                            >  2:1178089/1‑47 (MQ=255)

GCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACT  >  NC_000913/3453476‑3453739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A