Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,478,802	C→T	602 (TAG→TAA)	kefB ←	potassium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,478,802	0	C	T	100.0%	156.0 / NA	49	602 (TAG→TAA)	kefB	potassium:proton antiporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/25); total (24/25)

GATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCT  >  NC_000913/3478672‑3478935

gATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCaaa                                                                                                                               <  1:1275817/139‑1 (MQ=255)

                   ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGc                                                                                                            >  2:1420560/1‑139 (MQ=255)

                   ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGc                                                                                                            >  1:1634367/1‑139 (MQ=255)

                    tCAACAATATCAATATTATTTTCGCGCCACATGGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGAGTGCCATGTTTACCCTTTACTCAAATTCATCCCAGCCTTGCAGcg                                                                                                           >  1:885177/1‑138 (MQ=255)

                     caacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTg                                                                                                          >  1:1191467/1‑139 (MQ=255)

                     caacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTg                                                                                                          >  1:74854/1‑139 (MQ=255)

                            atcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGcgtcgt                                                                                                   >  1:1753543/1‑139 (MQ=255)

                                atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCtt                                                                                               >  2:225303/1‑139 (MQ=255)

                                    tattTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCATCTGGCGTCGTTCttgttg                                                                                           >  2:1264586/1‑139 (MQ=255)

                                           gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTAc                                                                                                                                    <  2:1312364/91‑1 (MQ=255)

                                           gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTAc                                                                                                                                    >  1:1312364/1‑91 (MQ=255)

                                           gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGt                                                                                                                 >  2:609528/1‑110 (MQ=255)

                                           gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGt                                                                                                                 <  1:609528/110‑1 (MQ=255)

                                           gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCa                                                                                    >  1:55866/1‑139 (MQ=255)

                                             gcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACg                                                                                  <  2:1753543/139‑1 (MQ=255)

                                              ccACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGc                                                                                 >  2:1620720/1‑139 (MQ=255)

                                                      ccATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAAt                                                                         <  1:225303/139‑1 (MQ=255)

                                                         ttGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCAttt                                                                                      >  1:54246/1‑123 (MQ=255)

                                                         ttGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCAttt                                                                                      <  2:54246/123‑1 (MQ=255)

                                                         ttGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCAc                                                                                   <  1:5891/126‑1 (MQ=255)

                                                         ttGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCAc                                                                                   >  2:5891/1‑126 (MQ=255)

                                                              tCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAAt                                                                         >  2:457910/1‑131 (MQ=255)

                                                              tCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAAt                                                                         <  1:457910/131‑1 (MQ=255)

                                                                cTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGt                                                               <  1:1785/139‑1 (MQ=255)

                                                                   cgcCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAg                                                                                                                     >  2:958526/1‑82 (MQ=255)

                                                                   cgcCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAg                                                                                                                     <  1:958526/82‑1 (MQ=255)

                                                                   cgcCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCCTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTcg                                                            >  2:1269076/1‑139 (MQ=255)

                                                                     ccTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTcgcg                                                          <  2:1634367/139‑1 (MQ=255)

                                                                        gACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCg                                                       <  1:1620720/139‑1 (MQ=255)

                                                                         aCAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCgg                                                      >  2:1415733/1‑139 (MQ=255)

                                                                                  gCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGtt                                                              <  1:839943/122‑1 (MQ=255)

                                                                                  gCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGtt                                                              >  2:839943/1‑122 (MQ=255)

                                                                                   cATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTgg                                            <  2:1004649/139‑1 (MQ=255)

                                                                                        tGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCga                                       <  2:1191467/139‑1 (MQ=255)

                                                                                            cccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAg                                                                                                                     >  1:1475509/1‑57 (MQ=255)

                                                                                            cccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAg                                                                                                                     <  2:1475509/57‑1 (MQ=255)

                                                                                                  aTAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                             <  1:957691/139‑1 (MQ=255)

                                                                                                       aCGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTa                        <  1:1420560/139‑1 (MQ=255)

                                                                                                           tttCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCgg                    >  1:1177761/1‑139 (MQ=255)

                                                                                                            ttCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                   <  2:1362351/139‑1 (MQ=255)

                                                                                                            ttCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                   <  2:117164/139‑1 (MQ=255)

                                                                                                            ttCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                   <  2:55866/139‑1 (MQ=255)

                                                                                                            ttCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                   <  1:1415733/139‑1 (MQ=255)

                                                                                                                    gCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTg           >  1:1029350/1‑139 (MQ=255)

                                                                                                                             aCCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                             >  2:4247/1‑112 (MQ=255)

                                                                                                                             aCCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                             <  1:4247/112‑1 (MQ=255)

                                                                                                                             aCCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGct  >  2:1576805/1‑139 (MQ=255)

                                                                                                                               ccTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCAc                                                                                   <  1:1023420/56‑1 (MQ=255)

                                                                                                                               ccTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGAt       <  1:1306472/132‑1 (MQ=255)

GATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCT  >  NC_000913/3478672‑3478935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A