Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,478,802	C→T	602 (TAG→TAA)	kefB ←	potassium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,478,802	0	C	T	100.0%	64.9 / NA	22	602 (TAG→TAA)	kefB	potassium:proton antiporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/12); total (10/12)

ATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCT  >  NC_000913/3478668‑3478937

aTCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACt                                                                                                                                     >  1:458890/1‑139 (MQ=255)

            cACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATccc                                                                                                                         >  2:447332/1‑139 (MQ=255)

                          aacacTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTgg                                                                                                           <  1:613941/139‑1 (MQ=255)

                           acaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGc                                                                                                          >  2:91490/1‑139 (MQ=255)

                                  caatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTc                                                                                                   <  1:447332/139‑1 (MQ=255)

                                       ttattTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttgtt                                                                                              >  2:525931/1‑139 (MQ=255)

                                                                  tCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACt                                                                                                                                     <  2:535199/73‑1 (MQ=255)

                                                                  tCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACt                                                                                                                                     >  1:535199/1‑73 (MQ=255)

                                                                    cTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGt                                                                 >  1:785604/1‑139 (MQ=255)

                                                                       cgcCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTcg                                                              >  1:393711/1‑139 (MQ=255)

                                                                          cTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGt                                                           <  2:69552/139‑1 (MQ=255)

                                                                                                  cGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGc                                                                                                          >  2:587197/1‑68 (MQ=255)

                                                                                                  cGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGc                                                                                                          <  1:587197/68‑1 (MQ=255)

                                                                                                      aTAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                               <  2:393711/139‑1 (MQ=255)

                                                                                                      aTAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                               <  2:508019/139‑1 (MQ=255)

                                                                                                      aTAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGCCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                               <  2:667208/139‑1 (MQ=255)

                                                                                                            cGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTc                                                                                                   >  1:585429/1‑65 (MQ=255)

                                                                                                            cGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTc                                                                                                   <  2:585429/65‑1 (MQ=255)

                                                                                                                ttCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                     <  1:91490/139‑1 (MQ=255)

                                                                                                                ttCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                     <  1:712317/139‑1 (MQ=255)

                                                                                                                     aTTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCTTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgcatgca                <  1:682359/139‑1 (MQ=255)

                                                                                                                                   ccTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGctct  >  2:268099/1‑139 (MQ=255)

ATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCT  >  NC_000913/3478668‑3478937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A