Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I2 R1
|
772 |
77.0 |
3769570 |
91.6% |
3452926 |
105.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,556,033 |
A→G |
Y273H (TAC→CAC) |
rtcA ← |
RNA 3'‑terminal phosphate cyclase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,556,033 | 0 | A | G | 100.0%
| 217.5
/ NA
| 62 | Y273H (TAC→CAC) | rtcA | RNA 3'‑terminal phosphate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/31); total (31/31) |
CACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACC > NC_000913/3555902‑3556153
|
caccacCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTc < 2:790982/139‑1 (MQ=255)
accGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACct < 1:89441/139‑1 (MQ=255)
cgcgATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACctct < 1:1865897/139‑1 (MQ=255)
ttGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCa > 2:1567029/1‑139 (MQ=255)
ggTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAAc < 1:723405/139‑1 (MQ=255)
cagATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGcc > 2:1858700/1‑139 (MQ=255)
ggCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAac < 2:178586/139‑1 (MQ=255)
ggCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAac > 1:1436456/1‑139 (MQ=255)
ggCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAac > 2:1332274/1‑139 (MQ=255)
gggATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCg > 1:342450/1‑139 (MQ=255)
gggCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCTCCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACt > 1:1330881/1‑139 (MQ=255)
gagCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCGGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACt > 1:447265/3‑139 (MQ=255)
ggCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACct > 2:1109941/1‑104 (MQ=255)
ggCGACCGTAAATTCCCCCGCGACCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACct < 1:1109941/104‑1 (MQ=255)
cGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAc < 1:1598924/139‑1 (MQ=255)
aCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACct > 1:984686/1‑100 (MQ=255)
aCCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACct < 2:984686/100‑1 (MQ=255)
cccGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCCACCGCCGCCGTGCATGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACcg > 2:1169032/1‑139 (MQ=255)
ccGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACcgac < 2:1330881/139‑1 (MQ=255)
cgcgCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCa > 2:634239/1‑106 (MQ=255)
cgcgCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCa < 1:634239/106‑1 (MQ=255)
cgcgCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACcgacg > 2:1347862/1‑139 (MQ=255)
gcgcCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTACTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACcgacga < 1:1347862/139‑1 (MQ=255)
cgcccgccAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCAcc > 2:1240744/1‑133 (MQ=255)
cgcccgccAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCAcc < 1:1240744/133‑1 (MQ=255)
aGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGc > 2:522722/1‑139 (MQ=255)
gcCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGtt < 1:239921/139‑1 (MQ=255)
gcCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGtt < 1:693876/139‑1 (MQ=255)
gcCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGtt < 1:1858700/139‑1 (MQ=255)
cATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCg < 2:1610119/139‑1 (MQ=255)
gTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACcgacga < 2:25968/111‑1 (MQ=255)
gTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACcgacga > 1:25968/1‑111 (MQ=255)
gTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACcga > 2:1712504/1‑108 (MQ=255)
gTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACcga < 1:1712504/108‑1 (MQ=255)
gTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACAACC‑CCGCACCGGCGCCCCTTTCCCCCGCCGCCAAAAAA‑CGTTCGGTGAATATTTCACtt > 1:1228746/1‑139 (MQ=255)
cAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGAc > 1:26409/1‑139 (MQ=255)
cAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTCTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGAc < 1:1332274/139‑1 (MQ=255)
gCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACtt > 1:1094185/1‑139 (MQ=255)
aGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACctct < 1:808486/48‑1 (MQ=255)
aGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACctct > 2:808486/1‑48 (MQ=255)
aGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCaa > 2:759870/1‑139 (MQ=255)
aGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCaa > 2:59241/1‑139 (MQ=255)
t‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCgaga < 1:1567029/139‑1 (MQ=255)
t‑CCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCgaga < 1:522722/139‑1 (MQ=255)
ccccACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCAc < 2:1067430/45‑1 (MQ=255)
ccccACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCAc > 1:1067430/1‑45 (MQ=255)
cACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcg‑cg > 2:838638/1‑75 (MQ=255)
cACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcg‑cg < 1:838638/75‑1 (MQ=255)
cACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTa > 1:683220/1‑139 (MQ=255)
aCCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCttt < 2:1098524/78‑1 (MQ=255)
aCCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACGGACG‑CGCttt > 1:1098524/1‑78 (MQ=255)
gccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTAc > 1:218176/1‑139 (MQ=255)
ccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTAcc < 2:218176/139‑1 (MQ=255)
ccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTAcc > 1:292839/1‑139 (MQ=255)
ccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTAcc < 2:1436456/139‑1 (MQ=255)
ccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTAcc < 1:1169032/139‑1 (MQ=255)
cgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAc > 1:602620/1‑66 (MQ=255)
cgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAc < 2:602620/66‑1 (MQ=255)
cgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACtt < 2:992463/112‑1 (MQ=255)
cgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACtt > 1:992463/1‑112 (MQ=255)
gCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTg < 2:875349/54‑1 (MQ=255)
gCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTg > 1:875349/1‑54 (MQ=255)
|
CACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATT‑CCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACG‑CGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACC > NC_000913/3555902‑3556153
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
