Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I2 R1
|
762 |
35.5 |
1545288 |
95.5% |
1475750 |
117.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,556,033 |
A→G |
Y273H (TAC→CAC) |
rtcA ← |
RNA 3'‑terminal phosphate cyclase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,556,033 | 0 | A | G | 100.0%
| 85.4
/ NA
| 25 | Y273H (TAC→CAC) | rtcA | RNA 3'‑terminal phosphate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (12/13); total (12/13) |
CTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCG > NC_000913/3555899‑3556166
|
cTCAACCACCGCGATATTGGCCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGt > 1:524113/1‑139 (MQ=255)
ccaccGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCAc < 1:245246/139‑1 (MQ=255)
accGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACct < 1:127347/139‑1 (MQ=255)
accGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACct < 2:524113/139‑1 (MQ=255)
gcgATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCtt < 2:370630/139‑1 (MQ=255)
atatTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCa > 2:237632/1‑139 (MQ=255)
aTGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCa > 1:50422/1‑139 (MQ=255)
gACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcg < 2:430600/139‑1 (MQ=255)
cccccGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCAcc < 2:50422/139‑1 (MQ=255)
ccccGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcg > 1:329341/1‑127 (MQ=255)
ccccGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcg < 2:329341/127‑1 (MQ=255)
ccggcgcCCGCTAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACcga > 2:448012/4‑139 (MQ=255)
ccGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACcgac > 1:17547/1‑139 (MQ=255)
tCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGt > 2:452530/1‑139 (MQ=255)
gTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGat < 1:445494/139‑1 (MQ=255)
cACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATAttt > 1:409838/1‑139 (MQ=255)
aCCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATAtttt > 2:604375/1‑139 (MQ=255)
gCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACtt < 2:217076/139‑1 (MQ=255)
ttCCCCCACCGCCGCCGTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCgag < 2:347989/139‑1 (MQ=255)
ccgccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTAtt > 1:724394/1‑139 (MQ=255)
gccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCa < 2:362653/111‑1 (MQ=255)
gccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCa > 1:362653/1‑111 (MQ=255)
ccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTAcc < 1:237632/139‑1 (MQ=255)
ccgccgTGCTTGCCAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTAcc > 2:654526/1‑139 (MQ=255)
cAGGTGGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTcg < 1:452530/139‑1 (MQ=255)
|
CTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCG > NC_000913/3555899‑3556166
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
