Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I2 R1 772 77.0 3769570 91.6% 3452926 105.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,818,584 G→A *275* (TAG→TAA)  dinD → DNA damage‑inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,818,5840GA100.0% 198.9 / NA 61*275* (TAG→TAA) dinDDNA damage‑inducible protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/33);  total (28/33)

TTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCTATTTTTTCTTA  >  NC_000913/3818457‑3818711
                                                                                                                               |                                                                                                                               
ttGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTaaa                                                                                                                      >  2:133916/1‑139 (MQ=255)
  gACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTaaaaa                                                                                                                    >  2:1145908/1‑139 (MQ=255)
         gTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCtttt                                                                                                             >  1:1732828/1‑139 (MQ=255)
         gTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCtttt                                                                                                             <  2:1717891/139‑1 (MQ=255)
               aGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGttga                                                                                                       <  2:61059/139‑1 (MQ=255)
               aGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGATGCCGACCCCGCAGGTCAGCATCAAGCACTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGttga                                                                                                       <  2:1119330/139‑1 (MQ=255)
                  tGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGttgattg                                                                                                    >  2:1225841/1‑139 (MQ=255)
                    aGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTgag                                                                                                  >  2:648515/1‑139 (MQ=255)
                     ggCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTgaga                                                                                                 <  1:878638/139‑1 (MQ=255)
                          aCCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACGGTGTAAAAATTACAGAGAAGAAATAACAGAAACTaaaa                                                                                                                     <  1:1127400/114‑1 (MQ=255)
                          aCCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACGGTGTAAAAATTACAGAGAAGAAATAACAGAAACTaaaa                                                                                                                     >  2:1127400/1‑114 (MQ=255)
                                cAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCg                                                                                      <  2:358556/139‑1 (MQ=255)
                                 aGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGt                                                                                     <  2:1732828/139‑1 (MQ=255)
                                     aCTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGc                                                                                 <  2:626095/139‑1 (MQ=255)
                                      cTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGcc                                                                                >  2:630989/1‑139 (MQ=255)
                                       ttGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCa                                                                               <  1:620237/139‑1 (MQ=255)
                                        tGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCaa                                                                              <  2:577363/139‑1 (MQ=255)
                                         ggCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAg                                                                             >  2:743742/1‑139 (MQ=255)
                                            ggAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTc                                                                          <  1:133916/139‑1 (MQ=255)
                                                  aTGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTg                                                                    <  2:1760807/139‑1 (MQ=255)
                                                   tGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAg                                                                                                                              >  2:77914/1‑80 (MQ=255)
                                                   tGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAg                                                                                                                              <  1:77914/80‑1 (MQ=255)
                                                   tGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGc                                                                   >  1:911666/1‑139 (MQ=255)
                                                    gCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCa                                                                  <  1:1145908/139‑1 (MQ=255)
                                                      cTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGt                                                                <  2:567806/139‑1 (MQ=255)
                                                          ggagTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATTCGTAAGCCAAGGTCCAg                                                                       <  2:1175526/128‑1 (MQ=255)
                                                          ggagTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAg                                                                       >  1:1175526/1‑128 (MQ=255)
                                                              ttGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTgaga                                                                                                 <  1:1685690/98‑1 (MQ=255)
                                                              ttGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTgaga                                                                                                 >  2:1685690/1‑98 (MQ=255)
                                                                gCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCg                                                                                      <  1:6212/107‑1 (MQ=255)
                                                                gCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCg                                                                                      >  2:6212/1‑107 (MQ=255)
                                                                gCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCa                                                                               >  2:1400186/1‑114 (MQ=255)
                                                                gCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCa                                                                               <  1:1400186/114‑1 (MQ=255)
                                                                gCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATg                                                      >  1:966533/1‑139 (MQ=255)
                                                                gCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATg                                                      >  2:1185263/1‑139 (MQ=255)
                                                                                aGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAAcaca                                                         >  2:730235/1‑120 (MQ=255)
                                                                                aGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAAcaca                                                         <  1:730235/120‑1 (MQ=255)
                                                                                  cATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCaa                                                                              >  1:467736/1‑97 (MQ=255)
                                                                                  cATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCaa                                                                              <  2:467736/97‑1 (MQ=255)
                                                                                  cATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACcttt                                    >  1:336404/1‑139 (MQ=255)
                                                                                   aTCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACctttc                                   <  1:1225841/139‑1 (MQ=255)
                                                                                       aGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAg                                                                                                                              >  1:222225/1‑44 (MQ=255)
                                                                                       aGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAg                                                                                                                              <  2:222225/44‑1 (MQ=255)
                                                                                       aGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGt                                                                     <  1:848727/101‑1 (MQ=255)
                                                                                       aGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGt                                                                     >  2:848727/1‑101 (MQ=255)
                                                                                        gCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTc                              <  1:1185263/139‑1 (MQ=255)
                                                                                            ttGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTccccc                          >  1:436920/1‑139 (MQ=255)
                                                                                             tGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCt                         <  1:648515/139‑1 (MQ=255)
                                                                                             tGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCt                         <  2:911666/139‑1 (MQ=255)
                                                                                                         tAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCt             >  1:1189960/1‑139 (MQ=255)
                                                                                                                 aCAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGt                                                                     <  1:1519044/75‑1 (MQ=255)
                                                                                                                 aCAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGt                                                                     >  2:1519044/1‑75 (MQ=255)
                                                                                                                 aCAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAacac                                                          >  1:1680275/1‑86 (MQ=255)
                                                                                                                 aCAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAacac                                                          <  2:1680275/86‑1 (MQ=255)
                                                                                                                   agagAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAAcaca                                                         >  1:1583371/1‑85 (MQ=255)
                                                                                                                   agagAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAAcaca                                                         <  2:1583371/85‑1 (MQ=255)
                                                                                                                    gagaAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCTATTTTTTCTTa  <  1:630989/139‑1 (MQ=255)
                                                                                                                       aagtaATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATGGCACCTTTCTTTTCCCCCt                         >  1:1209014/1‑113 (MQ=255)
                                                                                                                       aagaaATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCt                         <  2:1209014/113‑1 (MQ=255)
                                                                                                                          aaaTAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGt                                                                     >  1:1337572/1‑66 (MQ=255)
                                                                                                                          aaaTAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGt                                                                     <  2:1337572/66‑1 (MQ=255)
                                                                                                                               |                                                                                                                               
TTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAACCTATTTTTTCTTA  >  NC_000913/3818457‑3818711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A