Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,818,584	G→A	275 (TAG→TAA)	dinD →	DNA damage‑inducible protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,818,584	0	G	A	100.0%	69.6 / NA	23	275 (TAG→TAA)	dinD	DNA damage‑inducible protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/12); total (11/12)

CACACTTTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAA  >  NC_000913/3818451‑3818697

cacacTTTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGaa                                                                                                              >  2:156645/1‑139 (MQ=255)

cacacTTTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGaa                                                                                                              >  1:739905/1‑139 (MQ=255)

               gTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCtttt                                                                                               <  1:745146/139‑1 (MQ=255)

                   cAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTttgtt                                                                                           >  2:414170/1‑139 (MQ=255)

                                      cAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCg                                                                        <  1:156645/139‑1 (MQ=255)

                                               ggCAGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTgaga                                                                                   >  2:309910/1‑119 (MQ=255)

                                               ggCAGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTgaga                                                                                   <  1:309910/119‑1 (MQ=255)

                                                       cATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGt                                                       <  2:603241/139‑1 (MQ=255)

                                                                  agTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAacac                                            <  1:819193/139‑1 (MQ=255)

                                                                    ttGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTgaga                                                                                   <  2:375471/98‑1 (MQ=255)

                                                                    ttGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTgaga                                                                                   >  1:375471/1‑98 (MQ=255)

                                                                         gACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCt                                     <  2:277428/139‑1 (MQ=255)

                                                                                                  ttGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTCGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTa                                    <  2:576069/115‑1 (MQ=255)

                                                                                                  ttGGAAAACAGTGTAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTCGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTa                                    >  1:576069/1‑115 (MQ=255)

                                                                                                            gtgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAg                                                         >  1:668748/1‑84 (MQ=255)

                                                                                                            gtgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAg                                                         <  2:668748/84‑1 (MQ=255)

                                                                                                            gtgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGt                                                       >  2:841041/1‑86 (MQ=255)

                                                                                                            gtgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGt                                                       <  1:841041/86‑1 (MQ=255)

                                                                                                            gtgtAAAAATTACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTaa  >  1:313241/1‑139 (MQ=255)

                                                                                                                     ttACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACaa                                          <  2:268499/90‑1 (MQ=255)

                                                                                                                     ttACAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACaa                                          >  1:268499/1‑90 (MQ=255)

                                                                                                                       aCAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCa                                                    <  2:97063/78‑1 (MQ=255)

                                                                                                                       aCAGAGAAGAAATAACAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCa                                                    >  1:97063/1‑78 (MQ=255)

CACACTTTGACGTGGGTCGCAAGGTGAGGCAAACCATTCAGGAACTTGGCGGAACCATGCCTGAGGAGTTGCCGACCCCGCAGGTCAGCATCAAGCAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCCAAGGTCCAGGTGCAGTGAACACAATGGCTAAATATTGCACCTTTCTTTTCCCCCTCAGTTTTAA  >  NC_000913/3818451‑3818697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A