Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,970,077	G→A	349 (TAG→TAA)	wzzE →	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,970,077	0	G	A	100.0%	184.6 / NA	56	349 (TAG→TAA)	wzzE	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/27); total (29/27)

TATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑G‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAA  >  NC_000913/3969943‑3970206

tATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                                <  2:123480/139‑1 (MQ=255)

 aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac                                                                                                                               >  1:132874/1‑139 (MQ=255)

 aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac                                                                                                                               >  1:967866/1‑139 (MQ=255)

         ttGCGTACGCCGGAAGAACCGGCAAAACGCAATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑GCGGGGGCTGATCGGAGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACtactgc                                                                                                                       >  1:621380/1‑139 (MQ=255)

                cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAgc                                                                                                                >  2:652511/1‑139 (MQ=255)

                           ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATT‑ACCCGCCGTTGCTCGAAATAACaa                                                                                                                                <  1:1563589/111‑1 (MQ=255)

                           ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATT‑ACCCGCCGTTGCTCGAAATAACaa                                                                                                                                >  2:1563589/1‑111 (MQ=255)

                                aaaaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                                                                                <  2:132874/139‑1 (MQ=255)

                                               aCGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa                                                                                 >  2:1747900/1‑139 (MQ=255)

                                                cgtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAAt                                                                                <  1:1747900/139‑1 (MQ=255)

                                                 gtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATc                                                                               >  2:1521452/1‑139 (MQ=255)

                                                        cTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                                                                        <  2:1839495/139‑1 (MQ=255)

                                                           ccTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg                                                                           >  2:572536/1‑133 (MQ=255)

                                                           ccTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg                                                                           <  1:572536/133‑1 (MQ=255)

                                                           ccTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAg                                                                     >  2:832671/1‑139 (MQ=255)

                                                            cTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGt                                                                    <  2:525286/139‑1 (MQ=255)

                                                             tgatgaTTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                                                                   <  1:652511/139‑1 (MQ=255)

                                                                 gatTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                               <  1:841782/139‑1 (MQ=255)

                                                                 gatTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                               <  1:832671/139‑1 (MQ=255)

                                                                    tATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca                                                                                                                              <  2:760557/73‑1 (MQ=255)

                                                                    tATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca                                                                                                                              >  1:760557/1‑73 (MQ=255)

                                                                     aTGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGt                                                           <  1:1792589/139‑1 (MQ=255)

                                                                      tgcggggCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                                                                   >  1:805275/4‑130 (MQ=255)

                                                                      tgcggggCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                                                                   <  2:805275/127‑1 (MQ=255)

                                                                      tgcggggCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTa                                                          >  1:405511/4‑139 (MQ=255)

                                                                          gggCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                                >  1:761221/1‑65 (MQ=255)

                                                                          gggCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                                <  2:761221/65‑1 (MQ=255)

                                                                           ggCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTgg                                                     >  2:1785386/1‑139 (MQ=255)

                                                                            gCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGt                                                    <  1:1521452/139‑1 (MQ=255)

                                                                             cATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTa                                                   <  2:967866/139‑1 (MQ=255)

                                                                                tgtcgg‑gggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAcgc                                                <  1:982578/134‑1 (MQ=255)

                                                                                          ggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                                                                                >  2:605942/1‑82 (MQ=255)

                                                                                          ggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                                                                                <  1:605942/82‑1 (MQ=255)

                                                                                          ggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAg                                       >  2:658235/1‑139 (MQ=255)

                                                                                          ggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAg                                       >  1:245527/1‑139 (MQ=255)

                                                                                            cTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                              <  2:1356642/82‑1 (MQ=255)

                                                                                            cTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                              >  1:1356642/1‑82 (MQ=255)

                                                                                             tGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCa                                    >  1:1061558/1‑139 (MQ=255)

                                                                                             tGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCa                                    >  2:485250/1‑139 (MQ=255)

                                                                                                   gggCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGa                              >  2:1553326/1‑139 (MQ=255)

                                                                                                      cTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATgg                           <  2:405511/139‑1 (MQ=255)

                                                                                                         gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa                                                                                 >  2:1504387/1‑82 (MQ=255)

                                                                                                         gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa                                                                                 <  1:1504387/82‑1 (MQ=255)

                                                                                                         gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                               >  2:639397/1‑100 (MQ=255)

                                                                                                         gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                               <  1:639397/100‑1 (MQ=255)

                                                                                                         gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGgcgc                        >  2:1033743/1‑139 (MQ=255)

                                                                                                            tCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGt                     <  2:656260/139‑1 (MQ=255)

                                                                                                              gCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTg                   >  1:734034/1‑139 (MQ=255)

                                                                                                                   aaCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGa                                                                                                                  <  2:1228160/39‑1 (MQ=255)

                                                                                                                   aaCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGa                                                                                                                  >  1:1228160/1‑39 (MQ=255)

                                                                                                                    aCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                              <  2:55063/58‑1 (MQ=255)

                                                                                                                    aCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                              >  1:55063/1‑58 (MQ=255)

                                                                                                                        gccgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                                                                      >  2:27954/1‑62 (MQ=255)

                                                                                                                        gccgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                                                                      <  1:909642/62‑1 (MQ=255)

                                                                                                                        gccgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                                                                      <  1:27954/62‑1 (MQ=255)

                                                                                                                        gccgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                                                                      >  2:909642/1‑62 (MQ=255)

                                                                                                                         ccgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGtt        <  1:1785386/139‑1 (MQ=255)

                                                                                                                             tGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCa    >  1:75584/1‑139 (MQ=255)

                                                                                                                               cTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCaaa  >  2:303520/1‑139 (MQ=255)

                                                                                                                               cTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCaaa  >  1:924457/1‑139 (MQ=255)

TATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑G‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAA  >  NC_000913/3969943‑3970206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A